...% of their osmotically active water (OAW). Survival rates were further increased when rehydration was performed at 100% RH, rather than by direct contact with water. Two days at 98.2% RH resulted in a ∼30% loss of OAW, and dramatically increased the freeze tolerance of larvae to –10 and –15°C. The supercooling point...

... 2006 © The Company of Biologists Limited 2006 2006 crustaceans subterranean epigean freezing tolerance bound water crystallisation temperature inoculative freezing ice content cold acclimation glycogen Groundwater ecosystems are generally described as very poor in nutrients...

.... Here, we report a novel role for RCH by showing that RCH also increases freezing tolerance in an Antarctic midge, Belgica antarctica (Diptera, Chironomidae). The RCH response of B. antarctica was investigated under two distinct physiological states: summer acclimatized and cold acclimated. Summer...

... of the goldenrod gall fly Eurosta solidaginis Fitch (Diptera, Tephritidae). From September to December, hemolymph osmolality(455-926 mOsmol kg l -1 ) and freezing tolerance increased markedly in field-collected larvae. Chlorophenol Red was readily transported into the lumen of the foregut, the posterior portion...

...Douglas S. Neufeld; John P. Leader ABSTRACT Cells in freeze-tolerant tissues must survive substantial shrinkage during exposure to the hyperosmolarity that results as solutes are excluded from extracellular ice. We investigated the possibility that this hyperosmotic shock elicits an acute...

...Douglas S. Neufeld; John P. Leader ABSTRACT The ability of isolated Malpighian tubules from a freeze-tolerant insect, the New Zealand alpine weta ( Hemideina maori ), to withstand freezing was assessed by measuring post-freeze membrane potentials and rates of fluid secretion. The hemolymph of cold...

... blood cells hemolysis site-directed mutants ice recrystallization freezing tolerance cryopreservation Many marine fishes produce proteinaceous ‘antifreezes’ ( DeVries, 1983 ; Davies and Hew, 1990 ; DeVries and Cheng, 1992 ; Duman et al . 1993 ). These proteins have been classified...

... at 99% relative humidity and 15 °C. In water the nematodes are seeded by exogenous ice nucleation and a proportion are freezing tolerant. Ice formation appears to be restricted to the pseudocoel. The degree of freezing tolerance is dependent upon the age of the culture and its thermal history. P. davidi...