.... First, flow cytometry was used to monitor the peripheral blood immunophenotype of mice over the course of 36 days of hypoxic exposure. Second, hypoxic and normoxic mice were subjected to an adaptive immune challenge via keyhole limpet hemocyanin (KLH) or to an innate immune challenge via...

..., and prokaryotes as a PON source in sponge feeding. * Author for correspondence( [email protected] ) 12 8 2009 2009 Porifera coral reef filtration flow cytometry Sponges are ubiquitous aquatic, sessile, filter feeders. In some marine ecosystems, such as Antarctica or certain...

... of DNA during amphibian metamorphosis. In this study, nuclei were isolated from Xenopus laevis at various developmental stages. The nuclei were examined in both an unfixed state and a fixed state. The nuclei were stained with propidium iodide and analyzed by flow cytometry to determine their fluorescence...

... described in this paper is to use flow cytometry as a technique to monitor Ca 2+ uptake into Fluo-3-loaded membrane vesicles. This paper illustrates the utility of this technique for measuring ATP-dependent Ca 2+ uptake into hepatopancreatic basolateral membrane vesicles. Obstacles to progress in molecular...

...+ indicators Fluo-3 and Fura-2. Responses of lymphocytes to T-cell mitogen (phytohaemagglutinin, PHA), to B-cell mitogen (lipopolysaccharide, LPS) and to immunoglobulin (Ig) cross-linking with a monoclonal antibody to carp Ig were measured using flow cytometry. Both T-cell stimulation by PHA and B-cell...

... populations were characterised by light and electron microscopy and by flow cytometry. Fractions enriched in macrophages and neutrophilic granulocytes were subsequently analysed for phagocytic activity in vitro by quantification of the uptake of Escherichia coli bacteria or yeast cells, and for respiratory...