...-instar nymphs hatched from the eggs laid by females injected with dsRNA for eclosion hormone (dsEH), crustacean cardioactive peptide (dsCCAP) and dsOKA died at the expected time of ecdysis, indicating the crucial involvement of these genes in post-embryonic development. No phenotypes were observed upon...

... by injecting sensitive animals with the neuropeptide ecdysis-triggering hormone (ETH). Exposure to ETH stimulates the release of eclosion hormone (EH) which, in turn, activates a set of neurons containing crustacean cardioactive peptide (CCAP) by elevating their levels of intracellular cyclic GMP. We...

... of the behavior. Here, we show that high ecdysteroid levels suppress the onset of the behavioral sequence by inhibiting the development of competence to secrete ETH. In pharate pupae of Manduca sexta , Inka cells in the epitracheal glands normally develop competence to secrete ETH in response to eclosion hormone...

...James D. Baker; Susan L. McNabb; James W. Truman ABSTRACT In insects, ecdysis is thought to be controlled by the interaction between peptide hormones; in particular between ecdysis-triggering hormone (ETH) from the periphery and eclosion hormone (EH) and crustacean cardioactive peptide (CCAP) from...

... with a transcription-dependent decrease in spike threshold and the abrupt release of the previously stockpiled neuropeptide, eclosion hormone (EH). This report describes whole-cell patch-clamp recordings of acutely isolated VM cell somata made to identify membrane currents that may underlie the increase in VM cell...

...Stephen C. Gammie; James W. Truman ABSTRACT Three insect peptide hormones, eclosion hormone (EH), ecdysis-triggering hormone (ETH) and crustacean cardioactive peptide (CCAP), have been implicated in controlling ecdysis behavior in insects. This study examines the interactions between these three...

... indicates that eclosion hormone may initiate endocrine events leading to ecdysis through its action on epitracheal glands to cause the release of ecdysis-triggering hormone (ETH). Here, we report that direct exposure of epitracheal glands to eclosion hormone in vitro leads to secretion of ETH. The threshold...

... ecdysis triggering hormone (ETH), and the centrally located neurosecretory neurones, the VM neurones, which release eclosion hormone (EH). These two sets of endocrine cells mutally excite one another: EH acts on the Inka cells to cause the release of ETH. ETH, in turn, acts on the VM neurones to cause...

...Andrea Novicki; Janis C. Weeks ABSTRACT Each larval molt of Manduca sexta culminates in the sequential performance of pre-ecdysis (cuticle loosening) and ecdysis (cuticle shedding) behaviors. Both behaviors are thought to be triggered by the release of a peptide, eclosion hormone (EH), from brain...

...James W. Truman; Philip F. Copenhaver ABSTRACT Larval and pupal ecdyses of the moth Manduca sexta are triggered by eclosion hormone (EH) released from the ventral nervous system. The major store of EH activity in the latter resides in the proctodeal nerves that extend along the larval hindgut...

... to become competent to respond to the peptide eclosion hormone. Eclosion hormone is then released and acts directly on these muscles to induce rapid cell death which is morphologically and physiologically distinct from steroid-regulated atrophy. * Current address : Department of Physiology...