...@nencki.gov.pl ) 18 9 2007 © The Company of Biologists Limited 2007 2007 Blepharisma japonicum βγ-dimer G-protein phosducin 14-3-3 protein photophobic response protein phosphorylation Pdc–Gβγ interaction translocation confocal imaging cell fractionation FRET immunological...

..., California, USA) and all the other reagents were from Sigma (Steinheim, Germany). Untreated and isoproterenol-treated (10 μmol l –1 for 10 min) cells were fixed and processed for confocal imaging as previously described ( Wiejak et al.,2002 ). The primary antibodies were: rabbit polyclonal Ab against...