... impatiens workers exposed to 0°C for 75 min (inducing chill coma) and control bees maintained at a constant ambient temperature (28°C). We sequenced the 3′ end of mRNA transcripts (TagSeq) to quantify gene expression in thoracic tissue of bees at several time points (0, 10, 30, 120 and 720 min) following...

... temperature caused by a cold-induced spreading depolarization. The temperature where this occurs correlates with species biogeography. Spreading depression Cold tolerance Chill coma Lepidoptera CT min Carlsberg Foundation http://dx.doi.org/10.13039/501100002808 CF18-0940 and CF19-0472...

... to Na + /K + -ATPase thermal kinetics in the brain of Drosophila melanogaster . Critical thermal minimum Chill coma Sodium pump Enzyme kinetics Ionoregulation Glia Carlsbergfondet http://dx.doi.org/10.13039/501100002808 CF18-0940 CF19-0472 Natural Sciences...

..., and allow the study of bilateral auditory processing and the effect of reciprocal inhibition in central auditory neurons. Peltier element Chill coma Reciprocal inhibition Omega neurons For the analysis of bilateral auditory processing, insects such as crickets and bush-crickets...

...Mads Kuhlmann Andersen; Nikolaj Johannes Skole Jensen; R. Meldrum Robertson; Johannes Overgaard ABSTRACT When cooled, insects first lose their ability to perform coordinated movements (CT min ) after which they enter chill coma (chill coma onset, CCO). Both these behaviours are popular measures...

... and range shifts of bumblebees (genus Bombus ), the few measurements of thermal tolerance available for the group have relied on disparate measurement approaches. We describe a novel stereotypical behavior expressed by bumblebee individuals during entry into chill coma. This behavioral indicator of minimum...

... reproduction rather than to improve tolerance during cold exposure. Genome editing CRISPR-Cas9 Cold tolerance Chill coma Intrinsically disordered protein Insect In temperate, polar and alpine environments, insects and other small ectotherms risk internal ice formation and death when exposed...

...Anders Findsen; Johannes Overgaard; Thomas Holm Pedersen ABSTRACT Low temperature causes most insects to enter a state of neuromuscular paralysis, termed chill coma. The susceptibility of insect species to chill coma is tightly correlated to their distribution limits and for this reason...

... parasitic wasp, Aphidius colemani . Physiol. Entomol. 31 , 234 - 240 . Colinet H. , Lee S. F. , Hoffmann A. A. ( 2010 ). Knocking down expression of Hsp22 and Hsp23 by RNA interference affects recovery from chill coma in Drosophila melanogaster . J. Exp. Biol. 213 , 4146...

... reported for descriptive purposes are sample means ± s.e.m. When cooled at 0.25°C min –1 , female crickets entered chill coma at 2.3±0.1°C. All crickets exposed to 0°C were in chill coma when removed to room temperature for survival analysis. When assessed following 6 h of recovery at 25°C...

... phase are only beginning to be understood in insects. Hsp22 and Hsp23 are both upregulated during the recovery from prolonged chill coma in Drosophila melanogaster . This prompted us to investigate the functional significance of these modulations by testing whether expression of these two small Hsps...