... metabolism before feeding was 40% lower in hibernation cells compared with fed cells or active cells cultured at 37°C, thereby confirming the temperature independence of metabolic rate. The partial depression of circulating FFAs with feeding likely explains the incomplete restoration of insulin sensitivity...

... analysis: N.M.T.; Investigation: E.A.W.N.; Writing - original draft: E.A.W.N.; Writing - review & editing: N.M.T.; Visualization: N.M.T.; Supervision: N.M.T.; Project administration: N.M.T.; Funding acquisition: N.M.T. We would like to thank Kylie Colvin for help maintaining cell culture...

..., different adipokines, fatty acids, growth factors and PPAR agonists have been studied, providing relevant insights into which factors affect these processes and counterbalance AT dysregulation. In general, fish primary aSVF cell cultures also require the addition of a differentiation medium (DM) to induce...

... and suggestions. In this study, environmental hypoxia and the addition of lipids to cell culture media was sufficient to increase myoglobin in non-stimulated cultured Weddell seal skeletal muscle cells. The results suggest fundamental differences at the cellular level in myoglobin regulation in Weddell...

... ) 2 8 2004 © The Company of Biologists Limited 2004 2004 bivalve oyster Crassostrea gigas heart ionic current cell culture patch clamp bioassay cryopreservation There have been few studies on the electrophysiological properties of bivalve heart cells and particularly...

... they protect from both global and focal ischemia in vivo and cell culture models of ischemia/reperfusion injury in vitr o, the mechanism of protection is not well understood. Protein aggregation is part of the etiology of chronic neurodegenerative diseases such as Huntington's and Alzheimer's, and recent data...

... in suspension or proliferate after adhering to the surface of cell culture dishes. Phasecontrast microscopy revealed an extensive system of basal membrane invaginations, and Na + -K + -ATPase- and anionexchanger-like immunoreactivity could be localized in cell membranes. The cells were characterized by a high...

...Caroline Prem; Bernd Pelster SUMMARY A cell culture system has been developed in which swimbladder gas gland cells from the European eel ( Anguilla anguilla ) were cultured on a permeable support. Cells seeded on Anodisc 13 (Whatman) or Costar Transwell 13 mm membranes form a confluent cell layer...

... of Biologists 2000 gill cell culture filter insert chloride cell mitochondria-rich cell pavement cell transepithelial potential transepithelial resistance Na + /K + -ATPase activity Ca 2+ transport The branchial epithelium of freshwater fish is responsible for the active uptake of Na...

... of these interneurons in cell culture, a protocol for fast and selective labeling of their cell bodies was established. Prior to cell dissociation, the giant interneurons were backfilled through their axons in situ with a fluorescent dye (dextran tetramethylrhodamine). In primary cell cultures, the cell bodies of giant...

... and address for correspondence: Department of Child Health, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, Scotland (e-mail: [email protected] ) 3 11 1997 22 12 1997 © 1998 by Company of Biologists 1998 sweat glands cell culture stimulus...

... cell biology cell culture cell proliferation degeneration tendon injury tendon mechanics Tendons function as mechanical links, transmitting the force generated by a muscle to a distant bone and bringing about the movement of a segment of the limb. However, tendons in the legs and feet...

... Pierre Laurent for his advice on morphology. References Artursson , P. ( 1990 ). Epithelial transport of drugs in cell culture. I. A model for studying the passive diffusion of drugs over intestinal absorbtive (Caco-2) cells . J. pharmac. Sci. 79 , 476 – 482 . 10.1002/jps.2600790604...

... Institute, University of Zurich, August-Forel-Strasse 1, 8029 Zurich, Switzerland. † Author for correspondence (e-mail: [email protected] ). 25 06 1996 ©The Company of Biologists Limited 1996 muscle fiber isolation skeletal muscle fibers Rana pipiens cell culture...

.... , Guthrie , P. B. , Lawton , R. A. , Lee , R. E. and Kater , S. B. ( 1988 ). Characterization of cultured embryonic neurons from the snail Helisoma . In Cell Culture Approaches to Invertebrate Neurosciences (ed. D. Beadle , G. Lees and S. B. Kater ), pp. 85 – 108...

... in primary cell culture. 5-HT caused reversible reduction of both a rapidly activating A-type K + current and a relatively slowly activating K + current resembling a delayed rectifier-type conductance. 5-HT also reduced the magnitude of voltage-activated Ca 2+ influx in these cells. The functional...

.... , Montgomery , I. , Brown , U. M. O. , Smith , G. L. , Elder , H. Y. and Jenkinson , D. M. ( 1993b ). Investigation of stimulus–secretion coupling in equine sweat gland epithelia using cell culture techniques . J. exp. Biol . 183 , 179 – 199 . 10.1242/jeb.183.1.279 Wilson...

... that temperature may directly affect the function of neurosecretory cells. Cultured MNSCs showed a variety of morphologies. When a long primary axonal stump was retained after the dissociation process, a unipolar cell morphology was often promoted ( Figs 2A and 3B ; see also Kirchhof and Bicker, 1992...

... 6 1993 © 1993 by Company of Biologists 1993 leech Retzius neurons cholinergic response nicotinic receptor cell culture acetylcholine Leech Retzius neurons are a pair of serotonergic cells easily identifiable in each segmental ganglion. Retzius neurons in each midbody...

... . Gray , M. A. , Harris , A. , Coleman , L. , Greenwell , J. R. and Argent , B. E. ( 1989 ). Two types of chloride channels on duct cells cultured from human fetal pancreas . Am. J. Physiol. 257 , C240 – C251 . 10.1152/ajpcell.1989.257.2.C240 Grynkiewicz , G...