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Keywords: carbonic anhydrase
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J Exp Biol (2020) 223 (5): jeb212928.
Published: 12 March 2020
... knockdown or CRISPR/Cas9 knockout approaches were used to evaluate the roles of the two principal apical membrane acid excretory pathways, the Na + /H + exchanger (NHE3b; slc9a3.2 ) and the H + -ATPase ( atpv1aa ). Additionally, the role of HR cell cytosolic carbonic anhydrase (CAc) was investigated because...
Includes: Supplementary data
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J Exp Biol (2018) 221 (6): jeb151894.
Published: 21 March 2018
... for ammonia excretion and/or acid–base regulation, as supported by high expression levels of Rhesus-protein 1 (LpRh-1), cytoplasmic carbonic anhydrase (CA-2) and hyperpolarization-activated cyclic nucleotide-gated K + channel (HCN) compared with the PMPA and dorsal half-lamella. The half-lamellae displayed...
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J Exp Biol (2016) 219 (14): 2088–2097.
Published: 15 July 2016
.... pH Soluble adenylyl cyclase Carbonic anhydrase Carbon-concentrating mechanism Ocean acidification Osedax The vacuolar-type H + -ATPase (VHA) is a multi-subunit enzyme that is ubiquitous in eukaryotic cells. VHA uses the energy from ATP hydrolysis to drive H + transport against...
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J Exp Biol (2015) 218 (13): 2023–2029.
Published: 1 July 2015
... of Biologists Ltd 2015 Summary: Fluid absorption in the isolated midgut of adult female yellow fever mosquitoes is shown to spontaneously proceed at rates expected from in vivo urine excretion, and Na + /K + pumps, V-type H + pumps and carbonic anhydrase are shown to be important for the active...
Includes: Supplementary data
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J Exp Biol (2015) 218 (5): 675–683.
Published: 1 March 2015
... rates were enhanced in media buffered to pH 5 and decreased at pH 9.5. Inhibitor experiments, combined with enzyme activity measurements and mRNA expression analyses, further suggested that the excretion mechanism involves the participation of the V-type H + -ATPase, carbonic anhydrase, Na + /K...
Includes: Supplementary data
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J Exp Biol (2014) 217 (5): 663–672.
Published: 1 March 2014
... with carbonic anhydrases to act as a de facto sensor of pH and CO 2 . The two catalytic domains of sAC are related to HCO 3 − -regulated adenylyl cyclases from cyanobacteria, suggesting the cAMP pathway is an evolutionarily conserved mechanism for sensing CO 2 levels and/or acid/base conditions. Reports of sAC...
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J Exp Biol (2009) 212 (11): 1662–1671.
Published: 1 June 2009
... digestive environment in the midgut. The probable principle anion influencing the highly alkaline gut lumen is bicarbonate/carbonate. Bicarbonate/carbonate is regulated at least in part by the activity of carbonic anhydrases. Hence, we have focused attention on the carbonic anhydrases of the mosquito larva...
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J Exp Biol (2009) 212 (11): 1647–1661.
Published: 1 June 2009
...K. M. Gilmour; S. F. Perry SUMMARY Carbonic anhydrase (CA) is the zinc metalloenzyme that catalyses the reversible reactions of CO 2 with water. CA plays a crucial role in systemic acid–base regulation in fish by providing acid–base equivalents for exchange with the environment. Unlike air...
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J Exp Biol (2009) 212 (11): 1731–1744.
Published: 1 June 2009
... and posterior regions. The anterior segment is probably absorptive. The middle midgut secretes acid (pH<4.0), a process dependent on a carbonic-anhydrase-catalysed H + pool. Cells of the middle segment are alternately absorptive (apically amplified by ≈9-fold, basally amplified by >90-fold) and secretory...
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