... the basolateral membrane voltage of principal cells ( V bl ) from –75.2 mV to –88.2 mV and increased the cell input resistance from 363.7 kΩ to 516.3 kΩ. These effects of Ba 2+ reflect the block of K + channels that, apparently, are also permeable to Na + . Bumetanide (100μmol l –1 ) had no effect on TEP fluid...

... of the extract by reversed-phase liquid chromatography gave two fractions with antidiuretic effects. The more potent of these two factors was further characterized for its effects on the cryptonephric complex. Its antidiuretic effect is not inhibited by bumetanide, a drug that inhibits M. sexta diuretic hormone...

... to monitor unidirectional Cl − and Na + influx. Furthermore, net fluxes of NO 2 − , HCO 3 − , Cl − , Na + and water were recorded. NO 2 − uptake was inhibited by mucosal application of bumetanide (10 −4 mol l −1 ) but not DIDS (10 −3 mol l −1 ), suggesting that NO 2 − is transported across the intestine via...

... ) 15 4 1998 25 6 1998 © The Company of Biologists Limited 1998 membrane protein Na + −K + −2Cl − cotransport Na + –Cl − cotransport K + –Cl − cotransport cotransport bumetanide furosemide thiazide kidney choroid plexus neuron Gitelman’s syndrome Bartter’s syndrome...

... physiology suggests a model for tubular secretion of iso-osmotic fluid in most insects, in which ions are thought to enter basally either through a series of ion channels (Na + , K + and Cl − ) or through a bumetanide-sensitive Na + /K + /2Cl − cotransport. Apical fluxes are energised by a plasma-membrane H...

... not only in control solution but also in high-K + , Na + -free medium and reduced the transepithelial and the apical membrane potential differences but not the basal membrane potential difference or [K + ] i . Omitting Na + from the bathing medium, replacing Cl - by Br − or applying bumetanide (10 −5 mol l...