..., Lymnaea stagnalis L., and tested for tracer coupling with Lucifer Yellow CH and biocytin. The cells examined were the strongly electrically coupled neurones, visceral dorsal 1 (VD1) and right parietal dorsal 2 (RPD2) ( Boer et al . 1979 ; Benjamin and Pilkington, 1986 ), and the weakly coupled neurones...

... with fraction 46 from the last purification run, further confirming the sequence. In the first experiment, the freshly cut end of the visceral nerve trunk was incubated in a pool of biocytin (Molecular Probes, 4% in 0.05mol l −1 Tris/0.5mol l −1 KCl, pH7.5) for 4–7 days, during which time the biocytin...