..., indicative of the assembly of V 0 V 1 holoenzymes. These data show that a V-ATPase located in the apical plasma membranes of the secretory cells is a component of the apical `potassium pump' that has been identified previously by physiological approaches. The V-ATPase energizes the apical membrane...

... genes have been identified that encode proteins that are not part of the final V-ATPase complex yet are required for its assembly. These non-subunit Vma proteins function as dedicated V-ATPase assembly factors since their absence appears to inhibit assembly of the V-ATPase only. The assembly factors...

.... Similarly, some brown and red algae maintain an internal pH as low as 1 in their vacuoles. It was yeast genetics that allowed the identification of the special properties of individual subunits and the discovery of the factors that are involved in V-ATPase biogenesis and assembly. Null mutations in genes...

... between the V 1 and V o sectors is essential for ATP-driven proton transport, and this interaction is manipulated in vivo as a means of regulating V-ATPase activity. When yeast ( Saccharomyces cerevisiae ) cells are deprived of glucose for as little as 5 min, up to 75 % of the assembled V-ATPase complexes...

...Patricia M. Kane ABSTRACT Achieving an understanding of the biosynthesis, assembly and intracellular targeting of the vacuolar H + -ATPase is critical for understanding the distribution of acidic compartments and the regulation of organelle acidification. The assembly of the yeast vacuolar H...