.... These intertidal IBPs had high sequence similarity to fish and tick antifreeze glycoproteins and fish type II antifreeze proteins. Previously established classifiers based on machine learning techniques further predicted ice-binding activity in the majority of our newly identified putative IBPs. We investigated...

...John G. Duman; Jason E. Podrabsky; Jonathon H. Stillman; Lars Tomanek ABSTRACT Ice-binding proteins (IBPs) assist in subzero tolerance of multiple cold-tolerant organisms: animals, plants, fungi, bacteria etc. IBPs include: (1) antifreeze proteins (AFPs) with high thermal hysteresis antifreeze...

... of antifreeze action has yet to be determined. One current hypothesis, the ‘iceexclusion hypothesis’, suggests that antifreeze proteins act within epithelial membranes (skin, gills, gut) and block the entry of external ice crystals. This hypothesis was examined by measuring ice propagation temperatures across...