... cryoprotective agents. Antifreeze proteins have been identified that have the ability to confer cryoprotection in certain organisms. Antifreeze proteins are an evolutionary adaptation that contributes to the freeze resistance of certain fish, insects, bacteria and plants. These proteins adsorb to an ice...

...Philip K. Nickell; Sandra Sass; Dawn Verleye; Edward M. Blumenthal; John G. Duman SUMMARY To avoid freezing while overwintering beneath the bark of fallen trees, Dendroides canadensis (Coleoptera: Pyrochroidae) larvae produce a family of antifreeze proteins (DAFPs) that are transcribed in specific...

... antifreeze protein DSC differential scanning calorimetry TH thermal hysteresis The red flat bark beetle, Cucujus clavipes , has a broad latitudinal range in North America that extends from above the Arctic Circle (∼68°N) to North Carolina (∼35°N), and exists as a western subspecies ( C. c...

..., 95% of the last-instar nymphs emerged. N. arctica produce both glycerol and ice-binding factors (e.g. antifreeze protein) in response to low temperature. Hemolymph glycerol concentrations increased from 3 mmol l –1 to 930±114 mmol l –1 when temperatures were decreased from 4°C to –8°C, and N. arctica...

... in Alaska insects. Both populations produce glycerol, but the concentrations in Alaska larvae were much higher than in Indiana insects(∼2.2 and 0.5 mol l –1 , respectively). In addition, both populations produce antifreeze proteins. Interestingly, in the autumn both populations have the same approximate...

... that was due primarily to the retention of urea, but was not associated with accumulation of the polyols, sugars or amino acids commonly found in the cryoprotection systems of other animals. Also, Chrysemys picta (and Chelydra serpentina ) lacked both antifreeze proteins and ice-nucleating proteins, which...

...Ning Li; Cathy A. Andorfer; John G. Duman ABSTRACT Antifreeze proteins (AFPs) lower the non-equilibrium freezing point of water (in the presence of ice) below the melting point, thereby producing a difference between the freezing and melting points that has been termed thermal hysteresis...

... and in epidermal and hemolymph antifreeze proteins (AFPs). Seasonal cuticular modifications contribute to the inhibition of inoculative freezing since more cold-hardy larvae froze inoculatively when epicuticular waxes were removed with hexane and, in in vitro chamber experiments, cuticle patches...