... ng ml −1 . qPCR was used to capture changes in the expression of Kdm6b and Dmrt1 , using PowerUp SYBR Green Master Mix (Applied Biosystems). Three primers were used to characterize alternative splicing in Kdm6b . Kdm6b (+IR) was used to measure intron retention and Kdm6b (−IR) was used...

... hypergravity centrifugation to experimentally manipulate the loads experienced by Drosophila melanogaster . We examined effects of this manipulation on leg muscle alternative splicing of the sarcomere gene troponin T ( Dmel\up ; Fbgn0004169, herein referred to by its synonym TnT ), a process...

.... Alternative splicing Body weight sensing Mechanotransduction Muscle plasticity Scaling Tensegrity The design of most animal locomotor systems appears to be constrained mainly by body weight and associated considerations of durability. The effect of gravity, the one truly constant environmental...

...Miwa Suzuki; Hitomi Wakui; Takuya Itou; Takao Segawa; Yasuo Inoshima; Ken Maeda; Kiyoshi Kikuchi ABSTRACT This study investigated the expression of aquaporin 2 (AQP2) and its newly found alternatively spliced isoform (alternative AQP2) and the functions of these AQP2 isoforms in the cellular...

... and wrote the paper. S.R.P. designed the research and wrote the paper. Competing interests The authors declare no competing financial interests. 26 11 2013 27 1 2014 © 2014. Published by The Company of Biologists Ltd 2014 Transformer Doublesex RNAi Alternative splicing...

... (short, medium and long) were identified for the first time that are generated by alternative splicing of downstream coding and non-coding exons. Our work provides the first complete characterisation of the visual pigments of a parrot, perhaps the most colourful order of birds, and moreover suggests more...

...-bearing muscles receive mechanical feedback regarding body weight and consume ATP in order to generate forces sufficient to counteract gravity. Using rats, we examined how variation in body weight affected alternative splicing of fast skeletal muscle troponin T ( Tnnt3 ), a component of the thin filament...

..., arise by alternative splicing from very similar itp genes. ITP and ITPL are differentially distributed in (1) pars lateralis/retrocerebral complex neurosecretory cells(NSCs) containing both splice forms, (2) interneurons with either one of the splice forms, (3) hindgut-innervating abdominal ITP neurons...

...James H. Marden; Howard W. Fescemyer; Marjo Saastamoinen; Suzanne P. MacFarland; J. Cristobal Vera; Mikko J. Frilander; Ilkka Hanski SUMMARY A fundamental feature of gene expression in multicellular organisms is the production of distinct transcripts from single genes by alternative splicing...

... 7 2007 © The Company of Biologists Limited 2007 2007 forkhead box/winged helix myocyte nuclear factor (MNF) myogenesis thermal plasticity alternative splicing Myogenesis is a complex process involving the commitment of pluripotent stems cells to a myogenic fate, orchestrated...

...James H. Marden; Gail H. Fitzhugh; Mahasweta Girgenrath; Melisande R. Wolf; Stefan Girgenrath SUMMARY The flight muscles of Libellula pulchella dragonflies contain a mixture of six alternatively spliced transcripts of a single troponin T (TnT) gene. Here, we examine how intraspecific variation...