...( Rizzuto et al., 2000 ; Romagnoli et al., 2007 ; Szabadkai and Duchen, 2008 ). Moreover, key mitochondrial enzymes are calcium regulated, and so mitochondrial function is a calcium–sensitive process. In tubules, the measurement of mitochondrial calcium in vivo has been achieved using aequorin...

..., III, VI and VII. The Anopheles leucokinins and receptor were shown to be a functional receptor-ligand pair. All three Anopheles leucokinins caused a dose-dependent rise in intracellular calcium([Ca 2+ ] i ) when applied to S2 cells co-expressing the receptor and an aequorin transgene, with a potency...

..., isobutylmethyl xanthine (IBMX), before lysis and assay by competitive immunoassay according to the manufacturer’s instructions. Fig. 3. Measurement of intracellular [Ca 2+ ] by cell-specific expression of aequorin. The P{GAL4} transposon is capable of driving expression of any transgene that has a UAS...