... and Wood, 2008 ). Interference with production of H + by CA presumably reduces transport across both V-ATPase and the proposed 2Na + /H + exchanger. There is a substantive difference between our model of nuchal organ Na + transport and the Na + uptake model presented by the earlier whole-animal study...

... in distilled water, D. pagei survived without mortality, hemolymph osmolality and [Cl − ] increased briefly, stabilizing at initial values, while [Na + ] decreased continually. Expression of gill V-type H + -ATPase (V-ATPase), Na + /K + -ATPase and Na + /K + /2Cl − symporter genes was unchanged. In M. jelskii...

... ). The storage of protein, fat, glycogen and uric acid in the fat body and other tissues of mosquito larvae . J. Exp. Biol.   1942 , 56 - 77 . Zhuang , Z. , Linser , P. J. and Harvey , W. R. ( 1999 ). Antibody to H + V-ATPase subunit E colocalizes with portasomes in alkaline larval midgut...

... polychaete occurs via dentrically branched and well-vascularized branchiae, which exhibit a high abundance of three ammonia transporters and a Rhesus-like protein. AMTs Gill morphology V-ATPase cAMP Acid–base regulation HEA Ammonia (in this study NH 3 refers to gaseous ammonia, NH 4...

... expression levels of CeRhr-1 and CeRhr-2, V-ATPase and Na + /K + -ATPase also increased significantly in response to 1 mmol l −1 NH 4 Cl. Importantly, a functional expression analysis showed, for the first time, ammonia transport capabilities for CeRhr-1 in a phylogenetically ancient invertebrate system...

.... L.R., H.C. and A.D. co-wrote and co-edited the manuscript. Competing interests The authors declare no competing financial interests. 1 6 2014 2 9 2014 © 2014. Published by The Company of Biologists Ltd 2014 Na + /K + -ATPase V-ATPase Ion transport Epithelium...

... of Biologists Ltd 2014 V-ATPase Acid/base cAMP Carbonic anhydrase pH sensing Proton pump Cyclic adenosine monophosphate (cAMP) is the signaling molecule of one of the most versatile and evolutionarily conserved signaling pathways. cAMP is produced by adenylyl cyclase enzymes that use ATP...

... in body ammonia levels and to a downregulation of V-ATPase (subunit A) and Rh-protein mRNA. Further, a 2 day exposure to non-lethal ammonia concentrations (1 mmol l −1 ) caused a doubling of body ammonia levels and led to an increase in Rh-protein and Na + /K + -ATPase (α-subunit) mRNA expression levels...

...Bettina Schewe; Wolfgang Blenau; Bernd Walz SUMMARY Salivary gland cells of the blowfly Calliphora vicina have a vacuolar-type H + -ATPase (V-ATPase) that lies in their apical membrane and energizes the secretion of a KCl-rich primary saliva upon stimulation with serotonin (5-hydroxytryptamine...

... + /K + -ATPase activity, and expression of gill Na + /K + -ATPase α-subunit and V-ATPase B subunit mRNA during salinity (S) acclimation. We also characterize V-ATPase kinetics and the organization of transport-related membrane systems in the gill epithelium. Macrobrachium amazonicum strongly hyper...

... unknown and needs to be investigated. * Author for correspondence ( urmila@wsu.edu ) † Present address: Department of Biological Sciences, Wagner College, Staten Island, NY 10301, USA 12 10 2009 © 2010. V-ATPase acid secretion acid–base balance mosquito posterior midgut...

...Shai Saroussi; Nathan Nelson SUMMARY The life of every eukaryotic cell depends on the function of vacuolar H + -ATPase (V-ATPase). Today we know that V-ATPase is vital for many more physiological and biochemical processes than it was expected three decades ago when the enzyme was discovered...

... it appears to be established that the cation pump is a two-component system of a H + -transporting V-ATPase and a cation/nH + antiporter. After tracing the discovery of the V-ATPase as the energizer of K + /nH + antiport in the larval midgut of the tobacco hornworm Manduca sexta we show that research...

...Markus Huss; Helmut Wieczorek SUMMARY V-ATPases constitute a ubiquitous family of heteromultimeric, proton translocating proteins. According to their localization in a multitude of eukaryotic endomembranes and plasma membranes, they energize many different transport processes. Currently, a handful...

...Khaled H. Ahmed; Bernd Pelster SUMMARY Exposure of trout hepatocytes to hypertonicity induced a decrease in acridine orange (AO) fluorescence, indicating a corresponding decrease in pH inside the lumen of acidic compartments (pH L ). Pre-exposure of cells to the specific V-ATPase inhibitor...

... published data exist on the recta of anopheline larvae. To gain insight into the structure and functions of this organ in anopheline species, we used immunohistochemistry to compare the localization of three proteins [carbonic anhydrase (CA9),Na + /K + P-ATPase and H + V-ATPase] in the recta of anopheline...

...Jian Zuo; Sandra Vergara; Shinya Kohno; L. Shannon Holliday SUMMARY Vacuolar H + -ATPase (V-ATPase) is a fundamentally important enzyme in eukaryotic cells that is responsible for acidification of endocytic compartments. The B subunits of V-ATPases from mammals and tobacco hornworm have been shown...

...Bettina Schewe; Elmar Schmälzlin; Bernd Walz SUMMARY Blowfly salivary gland cells have a vacuolar-type H + -ATPase(V-ATPase) in their apical membrane that energizes secretion of a KCl-rich saliva upon stimulation with serotonin (5-hydroxytryptamine, 5-HT). We have used BCECF to study...

... vesicles (LMV) occurred by a vanadate- and thapsigargin-sensitive ATPase that was stimulated by a transmembrane proton gradient established by a co-localized V-ATPase associated with this organelle. In the present study, hepatopancreatic LMV from the American lobster Homarus americanus were prepared...

...Carsten Hille; Bernd Walz SUMMARY Cells of the dopaminergically innervated salivary ducts in the cockroach Periplaneta americana have a vacuolar-type H + -ATPase(V-ATPase) of unknown function in their apical membrane. We have studied whether dopamine affects intracellular pH (pH i ) in duct cells...