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Keywords: V-ATPase
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Journal Articles
J Exp Biol (2021) 224 (12): jeb242205.
Published: 11 June 2021
... a role for Na + /K + -ATPase, vacuolar H + -ATPase, Na + /H + and Na + /NH 4 + exchangers and a basolateral Na + /Cl − cotransporter. Bafilomycin Bufalin Ion transporters V-ATPase Ionoregulation Many species of hyperregulating adult crustaceans in fresh or brackish water typically...
Includes: Supplementary data
Journal Articles
J Exp Biol (2021) 224 (3): jeb233890.
Published: 4 February 2021
... in distilled water, D. pagei survived without mortality, hemolymph osmolality and [Cl − ] increased briefly, stabilizing at initial values, while [Na + ] decreased continually. Expression of gill V-type H + -ATPase (V-ATPase), Na + /K + -ATPase and Na + /K + /2Cl − symporter genes was unchanged. In M. jelskii...
Journal Articles
J Exp Biol (2017) 220 (17): 3172–3180.
Published: 1 September 2017
... of nutrients. Mosquito V-ATPase Na + /K + -ATPase Ion-selective microelectrodes Ionoregulation Gastric caecum Aedes aegypti is responsible for the spread of dengue, Zika fever, chikungunya and yellow fever, all of which infect millions of humans every year ( Tolle, 2009 ). The A. aegypti...
Journal Articles
J Exp Biol (2017) 220 (3): 425–436.
Published: 1 February 2017
... of adult E. complanata . (A) Western blot of total protein extract isolated from adult E. complanata . Application of anti-V-ATPase antibodies (subunit B specific) results in detection of a single protein of about 55 kDa (arrow) in the total protein extract. (B) Application of the same antibodies...
Journal Articles
J Exp Biol (2015) 218 (5): 675–683.
Published: 1 March 2015
... expression levels of CeRhr-1 and CeRhr-2, V-ATPase and Na + /K + -ATPase also increased significantly in response to 1 mmol l −1 NH 4 Cl. Importantly, a functional expression analysis showed, for the first time, ammonia transport capabilities for CeRhr-1 in a phylogenetically ancient invertebrate system...
Includes: Supplementary data
Journal Articles
J Exp Biol (2014) 217 (21): 3815–3822.
Published: 1 November 2014
.... L.R., H.C. and A.D. co-wrote and co-edited the manuscript. Competing interests The authors declare no competing financial interests. 1 6 2014 2 9 2014 © 2014. Published by The Company of Biologists Ltd 2014 Na + /K + -ATPase V-ATPase Ion transport Epithelium...
Journal Articles
J Exp Biol (2014) 217 (5): 663–672.
Published: 1 March 2014
... . Pastor-Soler   N. , Beaulieu   V. , Litvin   T. N. , Da Silva   N. , Chen   Y. , Brown   D. , Buck   J. , Levin   L. R. , Breton   S. ( 2003 ). Bicarbonate-regulated adenylyl cyclase (sAC) is a sensor that regulates pH-dependent V-ATPase recycling . J. Biol. Chem.   278...
Journal Articles
J Exp Biol (2012) 215 (18): 3242–3253.
Published: 15 September 2012
... in body ammonia levels and to a downregulation of V-ATPase (subunit A) and Rh-protein mRNA. Further, a 2 day exposure to non-lethal ammonia concentrations (1 mmol l −1 ) caused a doubling of body ammonia levels and led to an increase in Rh-protein and Na + /K + -ATPase (α-subunit) mRNA expression levels...
Journal Articles
J Exp Biol (2012) 215 (8): 1337–1345.
Published: 15 April 2012
...Bettina Schewe; Wolfgang Blenau; Bernd Walz SUMMARY Salivary gland cells of the blowfly Calliphora vicina have a vacuolar-type H + -ATPase (V-ATPase) that lies in their apical membrane and energizes the secretion of a KCl-rich primary saliva upon stimulation with serotonin (5-hydroxytryptamine...
Journal Articles
J Exp Biol (2010) 213 (22): 3894–3905.
Published: 15 November 2010
... + /K + -ATPase activity, and expression of gill Na + /K + -ATPase α-subunit and V-ATPase B subunit mRNA during salinity (S) acclimation. We also characterize V-ATPase kinetics and the organization of transport-related membrane systems in the gill epithelium. Macrobrachium amazonicum strongly hyper...
Journal Articles
J Exp Biol (2010) 213 (2): 295–300.
Published: 15 January 2010
... 2009 © 2010. V-ATPase acid secretion acid–base balance mosquito posterior midgut transepithelial potential An interesting and perplexing feature of the mosquito larval midgut is that the luminal compartments exhibit one of the highest pH values generated by a biological system...
Journal Articles
J Exp Biol (2009) 212 (11): 1604–1610.
Published: 1 June 2009
...Shai Saroussi; Nathan Nelson SUMMARY The life of every eukaryotic cell depends on the function of vacuolar H + -ATPase (V-ATPase). Today we know that V-ATPase is vital for many more physiological and biochemical processes than it was expected three decades ago when the enzyme was discovered...
Journal Articles
J Exp Biol (2009) 212 (11): 1611–1619.
Published: 1 June 2009
... it appears to be established that the cation pump is a two-component system of a H + -transporting V-ATPase and a cation/nH + antiporter. After tracing the discovery of the V-ATPase as the energizer of K + /nH + antiport in the larval midgut of the tobacco hornworm Manduca sexta we show that research...
Journal Articles
J Exp Biol (2009) 212 (3): 341–346.
Published: 1 February 2009
...Markus Huss; Helmut Wieczorek SUMMARY V-ATPases constitute a ubiquitous family of heteromultimeric, proton translocating proteins. According to their localization in a multitude of eukaryotic endomembranes and plasma membranes, they energize many different transport processes. Currently, a handful...
Journal Articles
J Exp Biol (2008) 211 (20): 3306–3314.
Published: 15 October 2008
...Khaled H. Ahmed; Bernd Pelster SUMMARY Exposure of trout hepatocytes to hypertonicity induced a decrease in acridine orange (AO) fluorescence, indicating a corresponding decrease in pH inside the lumen of acidic compartments (pH L ). Pre-exposure of cells to the specific V-ATPase inhibitor...
Journal Articles
J Exp Biol (2008) 211 (19): 3067–3076.
Published: 1 October 2008
... published data exist on the recta of anopheline larvae. To gain insight into the structure and functions of this organ in anopheline species, we used immunohistochemistry to compare the localization of three proteins [carbonic anhydrase (CA9),Na + /K + P-ATPase and H + V-ATPase] in the recta of anopheline...
Journal Articles
J Exp Biol (2008) 211 (7): 1102–1108.
Published: 1 April 2008
...Jian Zuo; Sandra Vergara; Shinya Kohno; L. Shannon Holliday SUMMARY Vacuolar H + -ATPase (V-ATPase) is a fundamentally important enzyme in eukaryotic cells that is responsible for acidification of endocytic compartments. The B subunits of V-ATPases from mammals and tobacco hornworm have been shown...
Journal Articles
J Exp Biol (2008) 211 (5): 805–815.
Published: 1 March 2008
...Bettina Schewe; Elmar Schmälzlin; Bernd Walz SUMMARY Blowfly salivary gland cells have a vacuolar-type H + -ATPase(V-ATPase) in their apical membrane that energizes secretion of a KCl-rich saliva upon stimulation with serotonin (5-hydroxytryptamine, 5-HT). We have used BCECF to study...
Journal Articles
J Exp Biol (2007) 210 (19): 3484–3493.
Published: 1 October 2007
... vesicles (LMV) occurred by a vanadate- and thapsigargin-sensitive ATPase that was stimulated by a transmembrane proton gradient established by a co-localized V-ATPase associated with this organelle. In the present study, hepatopancreatic LMV from the American lobster Homarus americanus were prepared...
Journal Articles
J Exp Biol (2007) 210 (8): 1463–1471.
Published: 15 April 2007
...Carsten Hille; Bernd Walz SUMMARY Cells of the dopaminergically innervated salivary ducts in the cockroach Periplaneta americana have a vacuolar-type H + -ATPase(V-ATPase) of unknown function in their apical membrane. We have studied whether dopamine affects intracellular pH (pH i ) in duct cells...