... trout gill cells. (A) Transepithelial resistance (TER) and (B) [ 3 H]PEG-400 flux across primary cultured single-seeded insert (SSI) preparations derived from rainbow trout gills. Measurements of TER and [ 3 H]PEG-400 flux were conducted at 24, 48, 72, 80, 96 and 108 h after seeding flask-cultured...

... perfused Malpighian tubules, the control transepithelial voltage ( V t ) was 19.4 mV (lumen-positive), and the transepithelial resistance ( R t ) was 6.37 kΩcm( Table 1 ). In principal cells studied by the methods of TEVC, the control basolateral membrane voltage( V bl ) was –75.2 mV, and the input...

... and mitochondria-rich cells in the approximate proportion in which they occur in vivo and to exhibit diffusive fluxes of Na + and Cl – similar to in vivo values, but does not exhibit active apical-to-basolateral transport of Na + . Transepithelial resistance and paracellular permeability are also known to increase...

... with Leibovitz’s L-15 medium on both surfaces (symmetrical conditions), transepithelial resistance (TER) stabilized at values as high as 34 kΩ cm 2 , indicative of electrically ‘tight’ epithelia. The density of MR cells, the surface area of their clusters and transepithelial potential (TEP; up to +8 mV basolateral...

... foetal bovine serum and glutamine) are trypsinized and the cells seeded onto the inserts. After 6 days of growth with L-15 medium on both surfaces (approximately isotonic to trout plasma), the cells form a tight epithelium as judged from a progressive rise in transepithelial resistance which reaches...