ABSTRACT The predicted rise of global temperatures is of major concern for ectotherms because of its direct impact on their behavior and physiology. As physiological performance mediates a species’ resilience to warming exposure, physiological plasticity could greatly reduce the susceptibility to climate change. We studied the degree to which Diplolaemus leopardinus lizards are able to adjust behavioral and physiological traits in response to short periods of temperature change. We used a split cross design to measure the acclimation response of preferred body temperature ( T p ), and the thermal performance curve of resting metabolic rate (RMR) and evaporative water loss (EWL). Our results showed that plasticity differs among traits: whereas T p and EWL showed lower values in warm conditions, the body temperature at which RMR was highest increased. Moreover, RMR was affected by thermal history, showing a large increase in response to cold exposure in the group initially acclimated to warm temperatures. The reduction of EWL and the increase in optimal temperature will give lizards the potential to partially mitigate the impact of rising temperatures in the energy cost and water balance. However, the decrease in T p and the sensitivity to the warm thermal history of RMR could be detrimental to the energy net gain, increasing the species’ vulnerability, especially considering the increase of heat waves predicted for the next 50 years. The integration of acclimation responses in behavioral and physiological traits provides a better understanding of the range of possible responses of lizards to cope with the upcoming climatic and environmental modifications expected as a result of climate change.

ABSTRACT Thermal dependence of growth and metabolism can influence thermal preference and tolerance in marine ectotherms, including threatened and data-deficient species. Here, we quantified the thermal dependence of physiological performance in neonates of a tropical shark species (blacktip reef shark, Carcharhinus melanopterus ) from shallow, nearshore habitats. We measured minimum and maximum oxygen uptake rates ( Ṁ O 2 ), calculated aerobic scope, excess post-exercise oxygen consumption and recovery from exercise, and measured critical thermal maxima (CT max ), thermal safety margins, hypoxia tolerance, specific growth rates, body condition and food conversion efficiencies at two ecologically relevant acclimation temperatures (28 and 31°C). Owing to high post-exercise mortality, a third acclimation temperature (33°C) was not investigated further. Acclimation temperature did not affect Ṁ O 2 or growth, but CT max and hypoxia tolerance were greatest at 31°C and positively associated. We also quantified in vitro temperature (25, 30 and 35°C) and pH effects on haemoglobin–oxygen (Hb–O 2 ) affinity of wild-caught, non-acclimated sharks. As expected, Hb–O 2 affinity decreased with increasing temperatures, but pH effects observed at 30°C were absent at 25 and 35°C. Finally, we logged body temperatures of free-ranging sharks and determined that C. melanopterus neonates avoided 31°C in situ . We conclude that C. melanopterus neonates demonstrate minimal thermal dependence of whole-organism physiological performance across a seasonal temperature range and may use behaviour to avoid unfavourable environmental temperatures. The association between thermal tolerance and hypoxia tolerance suggests a common mechanism warranting further investigation. Future research should explore the consequences of ocean warming, especially in nearshore, tropical species.

ABSTRACT Stress-induced hyperthermia (SIH) is characterised by a rise in body temperature in response to a stressor. In endotherms, SIH is mediated by the autonomic nervous system, whereas ectotherms must raise their body temperature via behavioural means by moving to warmer areas within their environment (behavioural thermoregulation). A recent study suggested that zebrafish ( Danio rerio ), an important model species, may move to warmer water in response to handling and confinement and thus exhibit SIH, which, if accepted, may have important practical and welfare implications. However, an alternative hypothesis proposed that the observed movements may be produced by avoidance behaviour rather than behavioural thermoregulation. Investigating the claims for SIH in zebrafish further, we conducted two experiments that extend the earlier study. The first experiment incorporated new conditions that considered fish behaviour in the absence of thermal variation, i.e. their null distribution, an important condition that was not performed in the original study. The second was a refined version of the experiment to reduce the numbers of fish and aid movement between areas for the fish. In contrast to the previous study, we saw no effect of handling or confinement on preference for warmer areas, and no evidence for SIH in either experiment. Instead, we observed a short-lived reduction in preference for warmer areas immediately post-stress. Our work suggests that zebrafish may not experience SIH, and claims regarding fish consciousness based on SIH may need to be revised.

ABSTRACT The energetics of processing a meal is crucial for understanding energy budgets of animals in the wild. Given that digestion and its associated costs may be dependent on environmental conditions, it is necessary to obtain a better understanding of these costs under diverse conditions and identify resulting behavioural or physiological trade-offs. This study examines the speed and metabolic costs – in cumulative, absolute and relative energetic terms – of processing a bloodmeal for a major zoonotic disease vector, the tsetse fly Glossina brevipalpis , across a range of ecologically relevant temperatures (25, 30 and 35°C). Respirometry showed that flies used less energy digesting meals faster at higher temperatures but that their starvation tolerance was reduced, supporting the prediction that warmer temperatures are optimal for bloodmeal digestion while cooler temperatures should be preferred for unfed or post-absorptive flies. 13 C-Breath testing revealed that the flies oxidized dietary glucose and amino acids within the first couple of hours of feeding and overall oxidized more dietary nutrients at the cooler temperatures, supporting the premise that warmer digestion temperatures are preferred because they maximize speed and minimize costs. An independent test of these predictions using a thermal gradient confirmed that recently fed flies selected warmer temperatures and then selected cooler temperatures as they became post-absorptive, presumably to maximize starvation resistance. Collectively these results suggest there are at least two thermal optima in a given population at any time and flies switch dynamically between optima throughout feeding cycles.

SUMMARY Many species hibernate to conserve energy during periods of low food and water availability. It has long been assumed that the optimal hibernation strategy involves long, deep bouts of torpor that minimize energy expenditure. However, hibernation has ecological (e.g. decreased predator avoidance) and physiological (e.g. sleep deprivation) costs that must be balanced with energy savings; therefore, individuals possessing sufficient energy reserves may reduce their use of deep torpor. We tested the hypothesis that energy (fat)availability influences temperature selection of two fat-storing bat species during hibernation. We predicted that individuals with small energy reserves would select colder temperatures for hibernation in order to minimize energy expenditure, while individuals with larger energy reserves would choose warmer temperatures to minimize the costs of hibernation. Results from our field experiment indicate that little brown myotis ( Myotis lucifugus )hibernating in warm microclimates were significantly heavier than individuals hibernating in cooler microclimates. To determine if energy availability was mediating this relationship, we limited fatty acid availability with mercaptoacetate (MA) and quantified its effect on torpid metabolic rate( TMR ) and thermal preference of big brown bats ( Eptesicus fuscus ). Administration of MA caused a 43% drop in TMR at 10°C and caused bats to choose significantly colder temperatures for hibernation. Our results suggest that fat-storing bats minimize torpor expression using both physiological and behavioral mechanisms.

SUMMARY The preferred body temperature of ectotherms is typically inferred from the observed distribution of body temperatures in a laboratory thermal gradient. For very small organisms, however, that observed distribution might misrepresent true thermal preferences. Tiny ectotherms have limited thermal inertia, and so their body temperature and speed of movement will vary with their position along the gradient. In order to separate the direct effects of body temperature on movement from actual preference behaviour on a thermal gradient, we generate a null model (i.e. of non-thermoregulating individuals)of the spatial distribution of ectotherms on a thermal gradient and test the model using parameter values estimated from the movement of nematodes( Caenorhabditis elegans ) at fixed temperatures and on a thermal gradient. We show that the standard lab strain N2, which is widely used in thermal gradient studies, avoids high temperature but otherwise does not exhibit a clear thermal preference, whereas the Hawaiian natural isolate CB4856 shows a clear preference for cool temperatures (∼17°C). These differences are not influenced substantially by changes in the starting position of worms in the gradient, the natal temperature of individuals or the presence and physiological state of bacterial food. These results demonstrate the value of an explicit null model of thermal effects and highlight problems in the standard model of C. elegans thermotaxis, showing the value of using natural isolates for tests of complex natural behaviours.