... to separate host (supernatant) and symbiont (pellet) fractions. Symbiodiniaceae were resuspended in 1% sodium dodecyl sulfate in 1× phosphate-buffered saline, and cell concentrations were determined in triplicate using a flow-cytometer (Guava easyCyte 5HT, Luminex, Austin, TX, USA) as described previously...

..., and marine phytoplankton – including Symbiodiniaceae – produce it prolifically ( Keller et al., 1989 ; Yoch, 2002 ; Lawson et al., 2019 ), as do corals in response to stress ( Hopkins et al., 2016 ). DMS is an antioxidant molecule that can penetrate thylakoid membranes and scavenge free radicals produced...

... ( Pocillopora acuta– Symbiodiniaceae) are mediated by light and/or temperature levels. In a 39 day experiment, elevated P CO 2 (962 versus 431 µatm P CO 2 ) had an interactive effect with midday light availability (400 versus 800 µmol photons m −2 s −1 ) and temperature (25 versus 29°C) on areal gross and net...