... techniques. A variety of channel proteins were identified and full-length sequence obtained for two of them, a Ca 2+ channel β subunit( Pp Ca V β) and a Shaker -like K + channel ( Pp K V 1). The location of the transcripts was confirmed by RT-PCR of total RNA isolated from individually selected and rinsed...

.... Activation and steady-state inactivation overlap and are markedly rightward-shifted compared with most Shaker -like currents (half-activation of 16.9±1.9 mV, N =7, half-inactivation of −35.4±3.0 mV, N =5). Recovery from inactivation was rapid (50±2.5 ms at −90 mV). Both currents were unaffected...

...Rohini Balakrishnan; Veronica Rodrigues ABSTRACT Mutations that affect the physiological properties of neurones or the development of neuronal circuits are likely to have profound effects on sensory and motor pathways. We have examined the effects of mutations in two loci -the Shaker complex...

...S. Singh; C. F. Wu ABSTRACT The larval muscle fibers of Drosophila show four outward K + currents in addition to the inward Ca 2+ current in voltage-clamp recordings. The Shaker (Sh) and the slowpoke (slo) mutations, respectively, eliminate the voltage-activated fast K + current (I A ) and the Ca 2...