... Trachemys scripta elegans , a species undergoing strong metabolic suppression when cold and anoxic. In warm normoxic turtles, free H 2 S was higher in red blood cells (RBCs) and kidney (∼9–10 µmol l −1 ) than in brain, liver and lung (∼1–2 µmol l −1 ). These values overall aligned with the tissue H 2 S...

...William R. Driedzic; Kathy A. Clow; Connie E. Short Energy metabolism was assessed in red blood cells (RBCs) from Atlantic cod and short-horned sculpin, two species that have markedly different levels of blood glucose. The objective was to determine whether the level of extracellular glucose has...

... and smaller red blood cells. However, these differences disappeared after 6 weeks of acclimation to normoxia at low altitude. The measured traits were also indistinguishable between the F 1 progeny of highland and lowland mice, indicating that there were no persistent differences in phenotype that could...

... conducted until the point of isotopic steady state with a diet. We kept six leopard sharks in captivity for 1250 days, measured their growth, and serially sampled plasma, red blood cells and muscle for stable carbon and nitrogen isotope analysis. A single-compartment model with first-order kinetics...

...Pia Koldkjær; Michael Berenbrink SUMMARY Haemoglobin concentrations in vertebrate red blood cells are so high that in human sickle cell disease a single surface amino acid mutation can result in formation of large insoluble haemoglobin aggregates at low oxygen levels,causing peculiar cell...

...A. J. Esbaugh; B. L. Tufts SUMMARY Carbonic anhydrase (CA) is a multi-functional enzyme that catalyzes the hydration/dehydration of carbon dioxide. In the red blood cell (rbc), CA is necessary to facilitate the transport of carbon dioxide out of the body. Results from earlier biochemical studies...

.... The objective of the current study was to determine if branchial CA activity in rainbow trout was the result of a general cytoplasmic CA isozyme, with kinetic properties,tissue distribution and physiological functions distinct from those of the red blood cell (rbc)-specific CA isozyme. Isolation and sequencing...

... anhydrase inhibition is different from that of all other known carbonic anhydrase inhibitors. The serum showed no inhibitory effect on carbonic anhydrase from human and bovine red blood cells. By applying the 18 O exchange reaction, it could be demonstrated that the presence of the carbonic anhydrase...

...Susan G. Lund; Matthew C. L. Phillips; Christopher D. Moyes; Bruce L. Tufts ABSTRACT The effects of cell age on protein synthesis were examined in the nucleated red blood cells of rainbow trout ( Oncorhynchus mykiss ). Total DNA content was unaffected by cell age, whereas total RNA content in young...

...Thomas Peters; Robert E. Forster; Gerolf Gros ABSTRACT The bicarbonate permeability of the plasma membrane of intact hagfish ( Myxine glutinosa ) red blood cells and the intracellular carbonic anhydrase activity of these cells were determined by applying the 18 O exchange reaction using a special...

... oxygen membrane transporter red blood cell regulation sickle cell disease K + /Cl − cotransport Na + /K + /Cl − cotransport Na + /H + exchange cell volume ion channel amino acid transport homeostasis In addition to the central role of red blood cells in gas transport...

...M. C. L. Phillips; C. D. Moyes; B. L. Tufts ABSTRACT The effects of cell age on metabolism in the nucleated red blood cells of rainbow trout ( Oncorhynchus mykiss ) were examined. Red blood cells were separated according to age using fixed-angle centrifugation. The mean erythrocyte haemoglobin...

... red cells. * Author for correspondence (e-mail: [email protected] ) 14 10 1998 07 12 1998 © 1999 by Company of Biologists 1999 volume regulation oxygenation acid activation amiloride Na + /H + exchange red blood cell flounder Platichthys flesus...

... of binding sites. * e-mail: [email protected] . 29 4 1994 ©The Company of Biologists Limited 1997 erythrocyte cortisol stress receptor starvation rainbow trout Oncorhynchus mykiss red blood cell The primary role of the erythrocyte is considered...

... Thamnophis elegans red blood cell The red cells of many lower vertebrates contain high concentrations of nucleoside triphosphates (NTPs: usually in the form of adenosine triphosphate, ATP, and guanosine triphosphate, GTP) which serve, in large part, to regulate hemoglobin allosterically...

... reversed the direction of the acid–base disequilibrium such that pHa increased by 0.059±0.016 units (mean ± S.E.M., N =6) during the stopflow period. When the CA activity of red blood cells (rbcs) was additionally inhibited using acetazolamide, the magnitude of the negative disequilibrium was increased...

...Steve F. Perry; Stephen G. Reid; Annika Salama ABSTRACT The effects of a 7-day period of daily physical stress (chasing until exhaustion) on the β -adrenergic response of the rainbow trout ( Oncorhynchus mykiss ) red blood cell (rbc) were examined in vitro . Physical stress was associated...

...) at the highest haemoglobin concentrations. The decrease in haemoglobin oxygen-affinity was attributed to the volume changes, since the intracellular pH did not decrease with increasing mean cellular haemoglobin concentration. Thus, the variations in red blood cell volume commonly observed during hypoxia may play...

... glucose concentration was more than adequate to support oxygen consumption. Glucose disappearance could not be accounted for by increases in lactate, red blood cell (RBC) glucose or RBC glycogen concentrations. Rates of 14 CO 2 production from [6- 14 C]glucose over a 2 h incubation period were less than 1...

... 5 1994 ©The Company of Biologists Limited 1994 Oncorhynchus mykiss trout carbonic anhydrase ventilation respiratory acidosis catecholamines exercise red blood cell CO 2 excretion It is now well established that severe, exhaustive exercise causes an increase...