...Barbara A. Katzenback; Helen A. Holden; Justin Falardeau; Christine Childers; Hanane Hadj-Moussa; Tyler J. Avis; Kenneth B. Storey Brevinin-1SY is the only described antimicrobial peptide (AMP) of Rana sylvatica . As AMPs are important innate immune molecules that inhibit microbes, this study...

...Jon P. Costanzo; M. Clara F. do Amaral; Andrew J. Rosendale; Richard E. Lee, Jr SUMMARY We investigated hibernation physiology and freeze tolerance in a population of the wood frog, Rana sylvatica , indigenous to Interior Alaska, USA, near the northernmost limit of the species' range. Winter...

...Christopher A. Dieni; Melanie C. Bouffard; Kenneth B. Storey SUMMARY The terrestrial anuran Rana sylvatica tolerates extended periods of whole-body freezing during the winter. Freezing survival is facilitated by extensive glycogen hydrolysis and distribution of high concentrations...

...Jon P. Costanzo; Richard E. Lee, Jr SUMMARY We tested the hypothesis that urea, an osmolyte accumulated early in hibernation, functions as a cryoprotectant in the freeze-tolerant wood frog, Rana sylvatica . Relative to saline-treated, normouremic (10 μmol ml –1 ) frogs, individuals rendered...

... frog ( Rana sylvatica ), a northern woodland species that hibernates terrestrially in sites where dehydration and freezing may occur. Wood frogs inhabiting an outdoor enclosure accumulated urea to 65 mmol l -1 in autumn and early winter, when soil moisture was scarce, but subsequently urea levels fell...

... frog) and a freeze-tolerant frog ( Rana sylvatica , wood frog). Ventral root (motoneuron) potentials were recorded from isolated, hemisected spinal cords of each species mounted in a sucrose-gap recording apparatus and superfused with HCO 3 - -buffered Ringer’s solution at room temperature (21 °C...

... of two local amphibians, Rana sylvatica and Ambystoma maculatum , to estimate the importance of each of these processes. We injected dye to check for water channels, measured oxygen partial pressures within egg masses to determine the influence of external water convection and lighting, measured oxygen...

...Jon P. Costanzo; Richard E. Lee, JR; Peter H. Lortz ABSTRACT In spring, the lowest temperature during freezing that can be survived by wood frogs ( Rana sylvatica ) from southern Ohio is approximately −3°C. We investigated whether the thermal limit of freeze tolerance in these frogs is regulated...