... differences in physiology between life stages, despite a potentially conserved cellular stress response. We used RNAseq to compare transcript expression in response to a cold stressor in Drosophila melanogaster larvae and adults and used RNA interference (RNAi) to test whether knocking down nine target genes...

... flagellomeres appear rich in trichoid and grooved sensilla, known to be associated with olfactory functions. Through an RNAi approach, topically delivered HhTAR1 dsRNA induced a 50% downregulation in gene expression after 24 h in H. halys 2nd instar nymphs. An innovative behavioural assay revealed that HhTAR1...

..., novel miR-26, which inhibits translation of the phosphofructokinase (PFK) gene by targeting the 3′ untranslated region (UTR) of pfk directly, thereby inhibiting molting and body length growth of the freshwater shrimp Neocaridina heteropoda . Lowering expression of pfk by RNA interference (RNAi) led...

... in response to and independent of heat stress. HSF-1 Heat stress Arthropod RNAi As the climate warms and organisms are pushed to their thermal limits, understanding the mechanistic basis underlying thermal tolerance is of increasing interest. Thermal stress elicits a near-universal cellular...

... changing as a result of recent advances in the large-scale sequencing of nematode genomes and the development of new techniques for their genetic manipulation. Notably, skin-penetrating gastrointestinal nematodes in the genus Strongyloides are now amenable to transgenesis, RNAi and CRISPR/Cas9-mediated...

... knockdown in locust flight muscle prevents extended flight activity. RNAi Fatty acid binding protein Insect flight Lipid transport For many centuries, locusts have inflicted severe damage to human populations in African and Asian countries. Every few years, when weather conditions...

...Alex M. Zimmer; Yihang K. Pan; Theanuga Chandrapalan; Raymond W. M. Kwong; Steve F. Perry ABSTRACT Loss-of-function technologies, such as morpholino- and RNAi-mediated gene knockdown, and TALEN- and CRISPR/Cas9-mediated gene knockout, are widely used to investigate gene function and its...

...Cristina Rodríguez-García; Jay D. Evans; Wenfeng Li; Belén Branchiccela; Jiang Hong Li; Matthew C. Heerman; Olubukola Banmeke; Yazhou Zhao; Michele Hamilton; Mariano Higes; Raquel Martín-Hernández; Yan Ping Chen ABSTRACT RNA interference (RNAi) is a post-transcriptional gene silencing mechanism...

.... RNAi Neurotoxicity Hippocampus Adeno-associated virus Tissue atrophy RNA interference (RNAi) is a popular technique for uncovering the function of individual proteins in cells or organisms ( Shan, 2010 ). As short hairpin RNAs (shRNAs) can be designed specifically for any structural gene...

... © 2015. Published by The Company of Biologists Ltd 2015 Summary: A novel protein from crayfish molar tooth is involved in calcium phosphate and carbonate precipitation and may prove important for bone and dental regeneration. Biomineralization Crustacean RNAi Calcium Hydroxyapatite...

... interests The author declares no competing financial interests. © 2015. Published by The Company of Biologists Ltd 2015 C. elegans RNAi Inherited The question of how much one generation affects the next has long been considered a cultural phenomenon, in which parents pass...

... that regulates male sex determination in XY beetles are not known. Parental RNAi-aided knockdown in expression of tra masculinizes genetic females (XX) that are fertile. The virgin females mated with these masculinized genetic females produced all female progeny. We present the genetic evidence to show...

... and studied in the forager stage of workers. Thus, we exploited the well-established RNA interference (RNAi) protocol for Vg knockdown to investigate its downstream effects on microRNA population in honey bee foragers' brain and fat body tissue. To identify microRNAs that are differentially expressed between...

... multidrug resistance-associated protein (dMRP, CG6214 ), a multidrug efflux transporter (MET, CG30344 ), and an organic anion transporting polypeptide 58Dc (OATP58Dc, CG3380 ). RNA interference (RNAi) knockdown and P-element insertion mutation of single OA transporter genes were used to evaluate...

... 2011. 2011 Penaeus monodon crustacean myostatin/GDF11 growth RNAi Cell growth, proliferation and homeostasis are controlled by a number of structurally related proteins belonging to the transforming growth factor-β (TGF-β) superfamily. Growth differentiation factor 8 (GDF-8), also...

.... , et al . ( 2007 ). A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila . Nature 448 , 151 - 156 . Doucet D. , Walker V. K. , Qin W. ( 2009 ). The bugs that came in from the cold: molecular adaptations to low temperatures in insects . Cell...

... lamina's major components, has previously been shown to bind several surface proteins of Plasmodium ookinetes. Here, using the recently developed RNAi technique in mosquitoes, we used a specific dsRNA construct targeted against the LANB2 gene (laminin γ1) of Anopheles gambiae to reduce its mRNA levels...

... sequences. However, the lack of a complete genetic tool box for mosquitoes remains a serious obstacle in our ability to study essential mosquito-specific mechanisms. Unlike Drosophila , very few null mutations for mosquito genes exist. The development of reverse-genetic analyses based on RNAi and transgenic...

...Anthony E. Brown; Andrea Crisanti; Flaminia Catteruccia SUMMARY Heritable RNA interference (RNAi) mediated by transgenes exhibiting dyad symmetry represents an important tool to study the function of genes expressed at late developmental stages. In this study, we determined whether...