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Keywords: RNAi
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Journal Articles
J Exp Biol (2022) 225 (11): jeb244063.
Published: 9 June 2022
... differences in physiology between life stages, despite a potentially conserved cellular stress response. We used RNAseq to compare transcript expression in response to a cold stressor in Drosophila melanogaster larvae and adults and used RNA interference (RNAi) to test whether knocking down nine target genes...
Includes: Supplementary data
Journal Articles
J Exp Biol (2021) 224 (8): jeb238816.
Published: 16 April 2021
... flagellomeres appear rich in trichoid and grooved sensilla, known to be associated with olfactory functions. Through an RNAi approach, topically delivered HhTAR1 dsRNA induced a 50% downregulation in gene expression after 24 h in H. halys 2nd instar nymphs. An innovative behavioural assay revealed that HhTAR1...
Includes: Supplementary data
Journal Articles
J Exp Biol (2020) 223 (13): jeb223529.
Published: 8 July 2020
..., novel miR-26, which inhibits translation of the phosphofructokinase (PFK) gene by targeting the 3′ untranslated region (UTR) of pfk directly, thereby inhibiting molting and body length growth of the freshwater shrimp Neocaridina heteropoda . Lowering expression of pfk by RNA interference (RNAi) led...
Includes: Supplementary data
Journal Articles
J Exp Biol (2020) 223 (3): jeb208611.
Published: 5 February 2020
... RNAi As the climate warms and organisms are pushed to their thermal limits, understanding the mechanistic basis underlying thermal tolerance is of increasing interest. Thermal stress elicits a near-universal cellular ‘heat shock response’, which has been characterized in a diversity...
Includes: Supplementary data
Journal Articles
J Exp Biol (2020) 223 (Suppl_1): jeb206482.
Published: 1 February 2020
... changing as a result of recent advances in the large-scale sequencing of nematode genomes and the development of new techniques for their genetic manipulation. Notably, skin-penetrating gastrointestinal nematodes in the genus Strongyloides are now amenable to transgenesis, RNAi and CRISPR/Cas9-mediated...
Journal Articles
J Exp Biol (2019) 222 (21): jeb203455.
Published: 6 November 2019
... knockdown in locust flight muscle prevents extended flight activity. RNAi Fatty acid binding protein Insect flight Lipid transport For many centuries, locusts have inflicted severe damage to human populations in African and Asian countries. Every few years, when weather conditions...
Journal Articles
J Exp Biol (2019) 222 (7): jeb175737.
Published: 4 April 2019
...Alex M. Zimmer; Yihang K. Pan; Theanuga Chandrapalan; Raymond W. M. Kwong; Steve F. Perry ABSTRACT Loss-of-function technologies, such as morpholino- and RNAi-mediated gene knockdown, and TALEN- and CRISPR/Cas9-mediated gene knockout, are widely used to investigate gene function and its...
Journal Articles
J Exp Biol (2018) 221 (19): jeb184606.
Published: 5 October 2018
...Cristina Rodríguez-García; Jay D. Evans; Wenfeng Li; Belén Branchiccela; Jiang Hong Li; Matthew C. Heerman; Olubukola Banmeke; Yazhou Zhao; Michele Hamilton; Mariano Higes; Raquel Martín-Hernández; Yan Ping Chen ABSTRACT RNA interference (RNAi) is a post-transcriptional gene silencing mechanism...
Journal Articles
J Exp Biol (2017) 220 (8): 1400–1404.
Published: 15 April 2017
...) were identified as molecular markers and early indicators of adverse tissue responses. Our findings also demonstrate that detrimental effects of high shRNA expression in hippocampal tissue can be monitored even before the onset of tissue degeneration. RNA interference (RNAi) is a popular technique...
Includes: Supplementary data
Journal Articles
J Exp Biol (2015) 218 (21): 3487–3498.
Published: 1 November 2015
... through an RNAi loss of function approach was attempted by Cq-M15 dsRNA administration. First, the Cq-M15 dsRNA sequence was compared with all known transcripts listed in C. quadricarinatus databases, in order to rule out non-specific silencing of non- Cq-M15 transcripts in the crayfish ( Yudkovski...
Includes: Supplementary data
Journal Articles
J Exp Biol (2015) 218 (1): 41–49.
Published: 1 January 2015
... interests The author declares no competing financial interests. © 2015. Published by The Company of Biologists Ltd 2015 C. elegans RNAi Inherited The question of how much one generation affects the next has long been considered a cultural phenomenon, in which parents pass...
Journal Articles
J Exp Biol (2014) 217 (10): 1653–1655.
Published: 15 May 2014
... that regulates male sex determination in XY beetles are not known. Parental RNAi-aided knockdown in expression of tra masculinizes genetic females (XX) that are fertile. The virgin females mated with these masculinized genetic females produced all female progeny. We present the genetic evidence to show...
Journal Articles
J Exp Biol (2013) 216 (19): 3724–3732.
Published: 1 October 2013
... and studied in the forager stage of workers. Thus, we exploited the well-established RNA interference (RNAi) protocol for Vg knockdown to investigate its downstream effects on microRNA population in honey bee foragers' brain and fat body tissue. To identify microRNAs that are differentially expressed between...
Includes: Supplementary data
Journal Articles
J Exp Biol (2012) 215 (15): 2601–2610.
Published: 1 August 2012
... multidrug resistance-associated protein (dMRP, CG6214 ), a multidrug efflux transporter (MET, CG30344 ), and an organic anion transporting polypeptide 58Dc (OATP58Dc, CG3380 ). RNA interference (RNAi) knockdown and P-element insertion mutation of single OA transporter genes were used to evaluate...
Journal Articles
J Exp Biol (2011) 214 (16): 2671–2677.
Published: 15 August 2011
... 2011. 2011 Penaeus monodon crustacean myostatin/GDF11 growth RNAi REFERENCES Aberle H. , Haghighi A. P. , Fetter R. D. , McCabe B. D. , Magalhaes T. R. , Goodman C. S. ( 2002 ). wishful thinking encodes a BMP type II receptor that regulates...
Journal Articles
J Exp Biol (2010) 213 (24): 4146–4150.
Published: 15 December 2010
... rate in the RNAi line ( act -GAL4/UAS- Hsp ) versus the control line ( act -GAL4/+) for Hsp22 (A) and Hsp23 (B). Mortality was assessed based on 150 males recovering for 24 h at 25°C after 12 h of cold stress at 0°C. Fig. 4. Mortality rate in the RNAi line (act-GAL4/UAS-Hsp) versus the control...
Journal Articles
J Exp Biol (2005) 208 (13): 2497–2502.
Published: 1 July 2005
... lamina's major components, has previously been shown to bind several surface proteins of Plasmodium ookinetes. Here, using the recently developed RNAi technique in mosquitoes, we used a specific dsRNA construct targeted against the LANB2 gene (laminin γ1) of Anopheles gambiae to reduce its mRNA levels...
Journal Articles
J Exp Biol (2003) 206 (21): 3835–3843.
Published: 1 November 2003
... sequences. However, the lack of a complete genetic tool box for mosquitoes remains a serious obstacle in our ability to study essential mosquito-specific mechanisms. Unlike Drosophila , very few null mutations for mosquito genes exist. The development of reverse-genetic analyses based on RNAi and transgenic...
Journal Articles
J Exp Biol (2003) 206 (11): 1817–1823.
Published: 1 June 2003
...Anthony E. Brown; Andrea Crisanti; Flaminia Catteruccia SUMMARY Heritable RNA interference (RNAi) mediated by transgenes exhibiting dyad symmetry represents an important tool to study the function of genes expressed at late developmental stages. In this study, we determined whether...