... and protein fluctuations . Biophys. J. 103 , 377 - 385 . 10.1016/j.bpj.2012.06.025 Gleason , L. U. and Burton , R. S. ( 2015 ). RNA-seq reveals regional differences in transcriptome response to heat stress in the marine snail Chlorostoma funebralis . Mol. Ecol. 24 , 610 - 627...

... acidification shows that while embryos are robust to low pH, the energetic cost may have negative carryover effects on swimming larvae. DESeq2 MZT Maternal provisioning RNA-seq Montipora capitata United States-Israel Binational Science Foundation 2016321 National Institute...

.... Transcriptomics Metabolomics Genomics RNA-seq Hypothesis testing A goal of comparative physiology is to understand the diverse mechanisms that allow animals to function and survive in their environments. Comparative physiologists (1) inquire vertically across levels of biological organization, (2...

... exposure is necessary for the development of key behaviors. Olfaction Swordtail fish Xiphophorus Boldness RNA-Seq Mate choice A major goal in evolutionary biology is to determine the mechanisms driving the origin and maintenance of reproductive isolation between species. Among closely...

... an adult-specific translational arrest mechanism that could explain divergent overwintering strategies across development. Chrysemys picta Comparative transcriptomics Reptile Ribosomal protein RNA-seq For almost every animal, oxygen is required for life, but variation in survival time...

... for multivariate analysis of gene expression data . Bioinformatics   21 , 2789 - 2790 . 10.1093/bioinformatics/bti394 Das , S. , Shyamal , S. and Durica , D. S. ( 2016 ). Analysis of annotation and differential expression methods used in RNA-seq studies in crustacean systems . Integr...

... significant alteration of their gene regulation and oxidative stress compared with their sister-mates operating under natural hive conditions. Honey bee RNA-seq Cage stress Imidacloprid Gene regulation Oxidative stress The honey bee Apis mellifera is an important eusocial insect...

... expressed transcripts from RNA-seq analyses of adult zebrafish whole eye from ZT4 and ZT16 time points. Fig. 2. Diurnal transcriptome of adult zebrafish whole eye. (A) Schematic of sample collection time points and lighting duration (14 h light:10 h dark) used in zebrafish maintenance. (B) Density...

..., such as RNA sequencing (RNA-seq). However, little attention has been given to how ecologically relevant field conditions may affect gene expression during diapause development because previous studies have focused on laboratory-reared and -maintained insects. To determine whether gene expression differs...

... Transcriptomics RNA-seq Biomineralization Shell formation (need 6-10) Thecosome pteropods, a group of marine planktonic shelled gastropods, contribute substantially to food webs and both carbon and carbonate flux in many pelagic ecosystems ( Berner and Honjo, 1981 ; Fabry and Deuser, 1991 ; Noji et...

... in the context of recent systems-level experiments that provide new insights into the relationship between gene expression and fitness. Environment Microarray Physiology RNA-seq Stress Temperature Transcriptomics The environment exerts a profound effect on biological systems, and strong...

... injury, neurological development and longevity are up-regulated in bees exposed to fluctuating temperatures during post-diapause quiescence. Chill injury Fluctuating thermal regime Megachile rotundata RNA-seq Temperature stress Insects have evolved the ability to cope with harsh, long...

... assembly of 108,645 contigs. Read alignment to the reference transcriptome was conducted with RNA-Seq by expectation maximization (RSEM) ( Li and Dewey, 2011 ). RSEM performs accurate transcript quantification when a reference genome is unavailable. It also allows for the inclusion of non-uniquely...

..., we use RNA-Seq to contrast gene expression during diapause with quiescence to identify transcriptional changes specific to the diapause response. We identify global trends in gene expression that show gradual convergence of diapause gene expression upon gene expression during quiescence. Functionally...