... , an eicosanoid inflammatory mediator. RNA interference (RNAi) using double-stranded RNAs specific to SePGDH or SePGR suppressed their expression levels. The RNAi treatment resulted in an excessive and fatal melanization of larvae even after a non-pathogenic bacterial infection. Phenoloxidase (PO) activity...

... by initial transient hyperpolarization followed by prolonged delayed depolarization. To explore the basis for these findings, we used RNA interference to selectively suppress expression of the genes encoding green opsin (GO1), UV opsin (UVO) or both. The hyperpolarizing component in UV-sensitive...

.... To further investigate J-domain protein function, ArHsp40 and ArHsp40-2 were each knocked down by RNA interference. Reductions in ArHsp40 and ArHsp40-2 had no effect on adult survival, time to release of cysts and nauplii from females and first-brood size. However, knockdown of both A. franciscana J-domain...

... developed an RNA interference (RNAi)-based method for clearing cuticular pigmentation which enables the use of whole-mount body appendages for RNA FISH (termed RNA-i-FISH). Silencing laccase2 or tyrosine hydroxylase in two leaf beetles species ( Chrysomela populi and Phaedon cochleariae ) cleared...

... This work was funded by a grant from the National Science Foundation (IOS-1656753 to E.P.E. and B.A.). References Agrawal , N. , Dasaradhi , P. V. N. , Mohmmed , A. , Malhotra , P. , Bhatnagar , R. K. and Mukherjee , S. K. ( 2003 ). RNA interference: biology, mechanism...

... of the role of the inhibitor of nuclear factor-κB, Cg-IκB2 . Anti-viral response RNA interference Inhibitor of NF-κB Marine bivalve Ostreid herpesvirus 1 In invertebrates, the defense against infectious microorganisms is driven by an innate-type immune system, which relies on highly...

...Gen Zhang; Li-Sheng He; Yue Him Wong; Li Yu; Pei-Yuan Qian ABSTRACT RNA interference (RNAi) provides an efficient and specific technique for functional genomic studies. Yet, no successful application of RNAi has been reported in barnacles. In this study, siRNA against p38 MAPK was synthesized...

... whether the circadian cycling of clock gene expression persists during diapause. We provide evidence that the major circadian clock genes continue to cycle throughout diapause and after diapause has been terminated. RNA interference (RNAi) was used to knock down the core circadian clock genes...

...-immunoreactive cell bodies at the medulla disrupted the photoperiodic regulation of diapause. However, gene suppression of pdf by RNA interference did not affect the photoperiodic response. These results suggest that the region containing PDF-immunoreactive somata is important for the photoperiodic response in R...

.... Published by The Company of Biologists Ltd 2013 queen–worker differentiation juvenile hormone (JH) RNA interference carbohydrate metabolism caste development insulin/insulin-like growth factor 1 signaling (IIS) morphological trait ovariole number body mass Eusocial insects, including...

... larvae. Both GPDH and GK were expressed in all developmental stages of S. exigua . RNA interference (RNAi) of either GPDH or GK significantly inhibited glycerol accumulation in the hemolymph of S. exigua . Larvae treated with RNAi for GPDH or GK exhibited a significant decrease in RCH capacity...

... organs and developmental stages of the tick during blood feeding were performed. The localization of ferritins in different organs was demonstrated through an indirect immunofluorescent antibody test. RNA interference (RNAi) was performed to evaluate the importance of ferritin in blood feeding...