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Keywords: RNA interference
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J Exp Biol (2020) 223 (21): jeb233221.
Published: 4 November 2020
... , an eicosanoid inflammatory mediator. RNA interference (RNAi) using double-stranded RNAs specific to SePGDH or SePGR suppressed their expression levels. The RNAi treatment resulted in an excessive and fatal melanization of larvae even after a non-pathogenic bacterial infection. Phenoloxidase (PO) activity...
Includes: Supplementary data
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J Exp Biol (2018) 221 (21): jeb189340.
Published: 1 November 2018
... by initial transient hyperpolarization followed by prolonged delayed depolarization. To explore the basis for these findings, we used RNA interference to selectively suppress expression of the genes encoding green opsin (GO1), UV opsin (UVO) or both. The hyperpolarizing component in UV-sensitive...
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J Exp Biol (2018) 221 (20): jeb189001.
Published: 19 October 2018
.... To further investigate J-domain protein function, ArHsp40 and ArHsp40-2 were each knocked down by RNA interference. Reductions in ArHsp40 and ArHsp40-2 had no effect on adult survival, time to release of cysts and nauplii from females and first-brood size. However, knockdown of both A. franciscana J-domain...
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J Exp Biol (2018) 221 (18): jeb185710.
Published: 25 September 2018
... developed an RNA interference (RNAi)-based method for clearing cuticular pigmentation which enables the use of whole-mount body appendages for RNA FISH (termed RNA-i-FISH). Silencing laccase2 or tyrosine hydroxylase in two leaf beetles species ( Chrysomela populi and Phaedon cochleariae ) cleared...
Includes: Supplementary data
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J Exp Biol (2018) 221 (6): jeb174094.
Published: 14 March 2018
... This work was funded by a grant from the National Science Foundation (IOS-1656753 to E.P.E. and B.A.). References Agrawal , N. , Dasaradhi , P. V. N. , Mohmmed , A. , Malhotra , P. , Bhatnagar , R. K. and Mukherjee , S. K. ( 2003 ). RNA interference: biology, mechanism...
Includes: Supplementary data
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J Exp Biol (2015) 218 (16): 2505–2509.
Published: 1 August 2015
...Gen Zhang; Li-Sheng He; Yue Him Wong; Li Yu; Pei-Yuan Qian ABSTRACT RNA interference (RNAi) provides an efficient and specific technique for functional genomic studies. Yet, no successful application of RNAi has been reported in barnacles. In this study, siRNA against p38 MAPK was synthesized...
Includes: Supplementary data
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J Exp Biol (2015) 218 (3): 412–422.
Published: 1 February 2015
... whether the circadian cycling of clock gene expression persists during diapause. We provide evidence that the major circadian clock genes continue to cycle throughout diapause and after diapause has been terminated. RNA interference (RNAi) was used to knock down the core circadian clock genes...
Includes: Supplementary data
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J Exp Biol (2013) 216 (23): 4347–4357.
Published: 1 December 2013
.... Published by The Company of Biologists Ltd 2013 queen–worker differentiation juvenile hormone (JH) RNA interference carbohydrate metabolism caste development insulin/insulin-like growth factor 1 signaling (IIS) morphological trait ovariole number body mass Eusocial insects, including...
Includes: Supplementary data
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Journal Articles