...Junji Yano; Russell Wells; Ying-Wai Lam; Judith L. Van Houten ABSTRACT Calcium ions (Ca 2+ ) entering cilia through the ciliary voltage-gated calcium channels (Ca V ) during the action potential causes reversal of the ciliary power stroke and backward swimming in Paramecium tetraurelia . How...

.... We demonstrate the device by recording action potentials in Paramecium and injecting a fluorescent dye into the cytosol. * Authors for correspondence ( [email protected] , [email protected] , [email protected] ) Competing interests...

...Sukanya Lodh; Junji Yano; Megan S. Valentine; Judith L. Van Houten ABSTRACT Paramecium cells swim by beating their cilia, and make turns by transiently reversing their power stroke. Reversal is caused by Ca 2+ entering the cilium through voltage-gated Ca 2+ (Ca V ) channels that are found...

...Taichi Toyoda; Yoshinori Hiramatsu; Toshiaki Sasaki; Yasuo Nakaoka SUMMARY Relationships between the thermo-sensitive response and membrane lipid fluidity were studied using a ciliated protozoan, Paramecium multimicronucleatum. Paramecium elicits a transient membrane depolarization in response...

...Yasuo Nakaoka; Takafumi Imaji; Masahiro Hara; Noboru Hashimoto SUMMARY The ciliated protozoan Paramecium spontaneously changes its swimming direction in the absence of external stimuli. Such behavior is based on resting potential fluctuations, the amplitudes of which reach a few mV. When...

...Wade E. Bell; Robin R. Preston; Junji Yano; Judith L. Van Houten SUMMARY Paramecium tetraurelia is attracted to acetate and biotin by swimming smoothly and fast up gradients of these attractants, and turning immediately and slowing down when leaving these stimuli. We use a group of mutants, each...

...Masaaki Iwamoto; Kazuyuki Sugino; Richard D. Allen; Yutaka Naitoh SUMMARY A fresh water protozoan Paramecium multimicronucleatum adapted to a given solution was found to swell until the osmotic pressure difference between the cytosol and the solution balanced the cytosolic pressure. The cytosolic...

...Jolanta Wiejak; Liliana Surmacz; Elzbieta Wyroba SUMMARY Evidence that dynamin is associated with the sequestration of the Paramecium β 2 -adrenergic receptor (βAR)immunoanalogue is presented. We previously reported a dramatic change in the distribution of βAR analogue in the subcellular fractions...

...Christopher R. Wood; Todd M. Hennessey SUMMARY Paramecium represents a simple, eukaryotic model system to study the cellular effects of some neuroactive drugs. They respond to the agonistβ,γ-methylene ATP with a transient depolarizing receptor potential,Ca 2+ -based action potentials and repetitive...

...Juan Bernal; Anita Mcilveen Kelsey; Barbara E. Ehrlich ABSTRACT Behavioral and electrophysiological experiments were made to examine the hypothesis that G-proteins modulate the voltage-dependent calcium channel in the marine ciliate Paramecium calkinsi . It was found that guanosine-5’-O-(3...

...A. M. Roberts; A. A. Al-Rudainy 21 3 1988 © 1988 by Company of Biologists 1988 Paramecium ciliary reversal electric field ion gates The ciliate Paramecium exhibits characteristic motile behaviour. When it is not feeding it normally swims in straight lines...

...Yasuo Nakaoka; Tohru Kurotani; Hirokazu Itoh ABSTRACT The localization of thermoreceptors in Paramecium , and the ionic basis of thermoreception, was investigated in posterior and anterior fragments of cells. Transverse section of the animals was used to obtain these fragments, which sealed up...

...Takashi Oka; Yasuo Nakaoka; Fumio Oosawa ABSTRACT When Paramecium are transferred from the solution to which they have been adapted to a solution of a different K + concentration, they initially change their swimming behaviour and then gradually recover normal swimming. This adaptation to the new...

...Munenori Noguchi; Hiroshi Inoué; Kazumi Kubo ABSTRACT The ciliary responses of Paramecium caudatum , extracted in Triton X-100 and glycerol, to the external application of ATP and various divalent cations were examined. Ciliary beating could not be reactivated, but changes in the pointing direction...

...T. Otter; E. D. Salmon ABSTRACT The behaviour of swimming Paramecium is markedly affected by hydro-static pressure (50-200atm, 1 atm = 101 325Pa). To investigate whether pressure might alter behaviour by acting directly on specific ion channels that mediate the behavioural responses, we examined...

...Boris Martinac; Hans Machemer ABSTRACT Effects of temperature on the electrical properties of the Paramecium membrane were investigated under constant current and voltage-clamp stimulation. With cells cultured at 18 °C, the resting potential was largely stable with experimental temperatures varied...

...Youko Satow; A. Don Murphy; Ching Kung ABSTRACT A depolarizing receptor potential produced by mechanical stimulation was studied in pawn mutants of Paramecium tetraurelia bathed in TEA solution. The potential was dependent upon the strength of the mechanical stimulation until a maximum response...