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Keywords: Ouabain
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Journal Articles
Journal:
Journal of Experimental Biology
J Exp Biol (2016) 219 (5): 626–630.
Published: 1 March 2016
...Kristin E. Spong; Tom R. Mazzetti; R. Meldrum Robertson ABSTRACT Spreading depression (SD) is associated with large changes in extracellular ion concentrations and can be induced by impairing mechanisms of K + ion homeostasis. We tested activity dependence of SD in the locust model of ouabain...
Journal Articles
Journal:
Journal of Experimental Biology
J Exp Biol (2015) 218 (13): 2023–2029.
Published: 1 July 2015
... filled with buffered NaCl (154 mmol l −1 plus 1 mmol l −1 HEPES) and bathed in mosquito saline with theophylline, fluid absorption was augmented (50.0±5.8 nl min −1 , N =12, ±s.e.m.). Concanamycin A (10 µmol l −1 ), ouabain (1 mmol l −1 ), and acetazolamide (1 mmol l −1 ) affected V te in different ways...
Includes: Supplementary data
Journal Articles
Journal:
Journal of Experimental Biology
J Exp Biol (2012) 215 (3): 497–507.
Published: 1 February 2012
... + /K + -ATPase inhibitor ouabain (10 mmol l –1 ; 21% reduction) and the vacuolar H + -ATPase inhibitor bafilomycin (5 μmol l –1 ; 21% reduction), and by the organic anions PAH (10 mmol l –1 ; 44% reduction) and probenecid (10 mmol l –1 ; 61% reduction, concentration-dependent inhibition). Transport...
Journal Articles
Journal:
Journal of Experimental Biology
J Exp Biol (2009) 212 (3): 373–377.
Published: 1 February 2009
... in supplying acid–base equivalents to the respective transporters. Furthermore, any apical processes proposed to be involved in alkali secretion or acid absorption must be Cl – independent and insensitive to DIDS,amiloride, Zn 2+ and ouabain. These results argue against the involvement of putative apical Cl...
Journal Articles
Journal:
Journal of Experimental Biology
J Exp Biol (2004) 207 (26): 4623–4631.
Published: 15 December 2004
... hemolymph-like salines. Although hemolymph-side ouabain did not affect this current, concanamycin significantly reduced I sc without altering preparation conductance, suggesting V-ATPase-driven Cl – absorption on the distal side of the posterior gill lamellae, as known to occur in diadromous crabs adapted...
Journal Articles
Journal:
Journal of Experimental Biology
J Exp Biol (2002) 205 (7): 897–904.
Published: 1 April 2002
... depolarized by 11±3 mV and 16±3 mV, respectively( N =11; 11). This conductance could also be inhibited by Ba 2+ , which depolarized a V bl of -71±5 mV by 9±3 mV ( N =5). The pump inhibitor ouabain (1 mmol l -1 ) depolarized V bl , but addition of furosemide to bath solution did not affect V bl . The[K...
Journal Articles
Journal:
Journal of Experimental Biology
J Exp Biol (2002) 205 (1): 71–77.
Published: 1 January 2002
... not differ significantly. Reducing the saline concentration of bath and perfusate from 30 ‰ to 20 ‰ or 10 ‰ increased significantly the TEPDs of these gills. TEPDs of gill 6 (representative of posterior gills) were reduced by 69±5 % and 60±5 % after perfusion with ouabain or BaCl 2 (5 mmol l –1 each...
Journal Articles
Journal:
Journal of Experimental Biology
J Exp Biol (1999) 202 (11): 1561–1570.
Published: 1 June 1999
... mol l −1 ouabain to unstimulated tubules depolarized the basolateral potential, increased the Na + concentration of the secreted fluid by 50–73 % and increased the fluid secretion rate by 10–19 %, consistent with an increased availability of intracellular Na + . We suggest that an apical vacuolar-type...
Journal Articles
Journal:
Journal of Experimental Biology
J Exp Biol (1997) 200 (13): 1913–1917.
Published: 1 July 1997
... with ouabain. The time to reach full depolarization ([K + ] o plateau) was three times longer in the anoxic brain than in normoxic controls, and the initial rate of K + leakage was reduced by approximately 70 %. Superfusing the brain before and during anoxia with the general adenosine receptor blocker...
Journal Articles
Journal:
Journal of Experimental Biology
J Exp Biol (1995) 198 (12): 2575–2580.
Published: 1 December 1995
... been blocked by ouabain revealed no change in K + permeability in crucian carp brain in response to anoxia. Furthermore, superfusing the brain of anoxic crucian carp with the K ATP channel blocker glibenclamide did not alter the efflux rate of K + after glycolysis had been inhibited with IAA...
Journal Articles
Journal:
Journal of Experimental Biology
J Exp Biol (1994) 197 (1): 421–428.
Published: 1 December 1994
... . J. exp. Biol. 61 , 357 – 377 . 10.1242/jeb.61.2.357 Maddrell , S. H. P. and Overton , J. A. ( 1988 ). Stimulation of sodium transport and fluid secretion by ouabain in an insect Malpighian tubule . J. exp. Biol. 137 , 265 – 276 . 10.1242/jeb.137.1.265...
Journal Articles
Journal:
Journal of Experimental Biology
J Exp Biol (1994) 192 (1): 285–290.
Published: 1 July 1994
... consumption temperature relationships Na + /K + -ATPase iodoacetic acid ouabain cycloheximide ethionine Carassius auratus goldfish We studied this problem by subjecting isolated goldfish hepatocytes to acute temperature changes and measuring the rate of total and function-related metabolism...
Journal Articles
Journal:
Journal of Experimental Biology
J Exp Biol (1993) 174 (1): 167–183.
Published: 1 January 1993
...J. A. K. Macvicker; P. F. Billingsley; M. B. A. Djamgoz ABSTRACT Na + /K + -ATPase activity was demonstrated in the midgut of Anopheles stephensi . More than 80% of the total ATPase activity was sensitive to inhibition by ouabain with an IC 50 of 4.5×10 −7 ±0.3×10 −7 mol l −1 and with maximal...
Journal Articles
Journal:
Journal of Experimental Biology
J Exp Biol (1988) 137 (1): 265–276.
Published: 1 July 1988
...S. H. P. Maddrell; J. A. Overton ABSTRACT Ouabain, at all concentrations higher than 2×l0 −7 mol l −1 , stimulates the rate at which the Malpighian tubules of the insect, Rhodnius , transport sodium ions and fluid into the lumen. An effect on paracellular movement of sodium ions is unlikely because...
Journal Articles
Journal:
Journal of Experimental Biology
J Exp Biol (1986) 121 (1): 43–54.
Published: 1 March 1986
... stops when sodium is replaced by choline in the perfusion fluid and is much reduced by treatment with ouabain, particularly when this is applied to the basolateral faces of the cells. These results suggest that ion absorption may be driven by an Na/K exchange pump. Cyclic AMP at 10 −2 moll −1 increases...