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Keywords: Na+/K+-ATPase
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Journal Articles
J Exp Biol (2021) 224 (3): jeb233890.
Published: 04 February 2021
... in distilled water, D. pagei survived without mortality, hemolymph osmolality and [Cl − ] increased briefly, stabilizing at initial values, while [Na + ] decreased continually. Expression of gill V-type H + -ATPase (V-ATPase), Na + /K + -ATPase and Na + /K + /2Cl − symporter genes was unchanged. In M. jelskii...
Journal Articles
J Exp Biol (2017) 220 (21): 3997–4006.
Published: 01 November 2017
... in the activity of basolateral Na + /K + -ATPase (NKA) and apical V-type H + -ATPase (VA) and (2) region-specific Na + and K + flux rates. Both indicate a prominent role for the hindgut (rectum) in K + reabsorption. MTs also exhibit region-specific differences in Na + and K + flux rates that are coupled...
Journal Articles
J Exp Biol (2017) 220 (17): 3172–3180.
Published: 01 September 2017
... + -ATPase and Na + /K + -ATPase is altered when larvae are reared in brackish water (30% seawater) relative to freshwater. Measurements of luminal Na + and K + concentrations also show a 5-fold increase in Na + /K + ratio in the caecal lumen in larvae reared in brackish water relative to freshwater, whereas...
Journal Articles
J Exp Biol (2016) 219 (9): 1346–1355.
Published: 01 May 2016
... and trimeric forms. AeAmt1 was immunolocalized to the basal side of the anal papillae epithelium where it co-localized with Na + /K + -ATPase. Ammonium concentration gradients were measured adjacent to anal papillae using the scanning ion-selective electrode technique (SIET) and used to calculate ammonia...
Journal Articles
J Exp Biol (2015) 218 (13): 2015–2022.
Published: 01 July 2015
... and presence of added ambient nitrite to decipher the cellular metabolism and effects of nitrite at basal and elevated concentrations under different oxygen regimes. We also tested hypotheses concerning the influence of nitrite on branchial nitric oxide synthase ( NOS ), Na + /K + -ATPase ( nka ) and heat...
Journal Articles
J Exp Biol (2015) 218 (10): 1467–1470.
Published: 15 May 2015
... ). Methods for non-lethal gill biopsy and measurement of Na + ,K + ATPase activity . Can. J. Fish. Aquat. Sci.   50 , 656 - 658 . 10.1139/f93-075 Perry , S. F. , Rivero-Lopez , L. , McNeil , B. and Wilson , J. M. ( 2006 ). Fooling a freshwater fish: how dietary salt transforms...
Journal Articles
J Exp Biol (2015) 218 (5): 675–683.
Published: 01 March 2015
... that ammonia is at least partially excreted via the hypodermis. Starvation reduced the ammonia excretion rates by more than half, whereas mRNA expression levels of the Rhesus protein CeRhr-2, V-type H + -ATPase (subunit A) and Na + /K + -ATPase (α-subunit) decreased correspondingly. Moreover, ammonia excretion...
Includes: Supplementary data
Journal Articles
J Exp Biol (2014) 217 (21): 3815–3822.
Published: 01 November 2014
... that projected onto the hindgut and posterior midgut. Application of a cockroach AST to the semi-isolated hindgut of larval C. riparius led to dose-dependent inhibition of muscle contractions with an EC 50 of ~10 nmol l −1 and a decrease in rectal K + reabsorption resulting from reduced rectal Na + /K + -ATPase...
Journal Articles
J Exp Biol (2014) 217 (13): 2309–2320.
Published: 01 July 2014
... as an osmoconformer in seawater). Additionally, expression of ion transporters and supporting enzymes that typically undergo upregulation during salinity transfer in osmoregulatory gills (i.e. Na + /K + -ATPase, carbonic anhydrase, Na + /K + /2Cl − cotransporter, V-type H + -ATPase and arginine kinase) were generally...
Includes: Supplementary data
Journal Articles
J Exp Biol (2013) 216 (18): 3433–3441.
Published: 15 September 2013
... , A. coluzzii and A. merus , as well as F1 progeny from reciprocal crosses of A. merus and A. coluzzii . Additionally, using immunohistochemistry, we compared the location of three ion regulatory proteins (Na + /K + -ATPase, carbonic anhydrase and Na + /H + -antiporter) in the recta of A. coluzzii and A. merus...
Includes: Supplementary data
Journal Articles
J Exp Biol (2013) 216 (18): 3531–3541.
Published: 15 September 2013
... was supported by a grant from the Ministry of Education, Science and Technological Development, Republic of Serbia [grant no. 173027]. REFERENCES Anderson   T. R. , Huguenard   J. R. , Prince   D. A. ( 2010 ). Differential effects of Na + -K + ATPase blockade on cortical layer V neurons...
Journal Articles
J Exp Biol (2012) 215 (4): 642–652.
Published: 15 February 2012
... individuals (141 mmol l –1 vs 134 mmol l –1 ), but the hematocrit remained unchanged. In seawater-acclimated individuals, branchial Na + /K + -ATPase (NKA) activity was higher by 75%. Western blot analysis indicated that the abundance of the NKA α-subunit and a Na + /K + /2Cl – cotransporter (NKCC1) were...
Journal Articles
J Exp Biol (2012) 215 (3): 497–507.
Published: 01 February 2012
... by locust Malpighian tubules exhibits a similar pharmacological profile, albeit with markedly higher concentrations of CPR being reached in S. gregaria . Immunolocalization of the Na + /K + -ATPase α-subunit in S. gregaria revealed that this transporter is abundantly expressed and localized to the basal...
Journal Articles
J Exp Biol (2011) 214 (23): 3992–3999.
Published: 01 December 2011
... inhibitors of the Na + /K + -ATPase and NKCC co-transporters, respectively, altered ion absorption or secretion by the anal papillae (Figs 3 and 4 ). The anal papillae of larval mosquitoes are organs that permit survival in dilute freshwater habitats by actively absorbing NaCl and therefore...
Journal Articles
J Exp Biol (2011) 214 (14): 2435–2442.
Published: 15 July 2011
... control levels over time. Gill H + -ATPase activity increased by more than 45% 14 days after exposure to freshwater, whereas H + -ATPase mRNA levels were not affected by the salinity change. Within 4 days of freshwater exposure, gill Na + /K + -ATPase activity increased ∼43% over control levels, remaining...
Journal Articles
J Exp Biol (2011) 214 (13): 2164–2174.
Published: 01 July 2011
... organism is reduced by more than 90%. Enzymes embedded in the plasma membrane, such as the Na + /K + -ATPase, may be even more susceptible to the cold because of thermal effects on the lipid bilayer. Accordingly, adaptive changes in response to the cold may include adjustments to the enzyme...
Includes: Supplementary data
Journal Articles
J Exp Biol (2010) 213 (22): 3894–3905.
Published: 15 November 2010
... + /K + -ATPase activity, and expression of gill Na + /K + -ATPase α-subunit and V-ATPase B subunit mRNA during salinity (S) acclimation. We also characterize V-ATPase kinetics and the organization of transport-related membrane systems in the gill epithelium. Macrobrachium amazonicum strongly hyper...
Journal Articles
J Exp Biol (2010) 213 (18): 3150–3160.
Published: 15 September 2010
... environmental ammonia (HEA; 1 and 5 mmol l –1 NH 4 HCO 3 ) and evaluated the patterns of ammonia excretion and gill Rh mRNA and protein expression. Gill H + -ATPase , NHE1 , NHE2 , NHE3 , Na + /K + -ATPase ( NKA ), Na + /K + /2Cl – co-transporter ( NKCC1 ) mRNA, H + -ATPase activity, NKA protein and activity...
Journal Articles
J Exp Biol (2010) 213 (3): 418–425.
Published: 01 February 2010
... ). Secretory K + (Rb + ) fluxes exceeded influxes and were inhibited by the Na + /K + -ATPase inhibitor ouabain and the K + channel blocker cesium. Western blot analysis showed that Na + /K + -ATPase α-subunit protein was more highly expressed in the epipodite of lobsters acclimated to 20 p.p.t. compared...
Journal Articles
J Exp Biol (2010) 213 (2): 210–224.
Published: 15 January 2010
... and gill – including changes in amino acid and inositol (i.e. osmolyte) metabolism, energy metabolism and proteins related to transcription, translation and protein degradation. Overall, leopard sharks employ a strategy of maintaining plasma urea, ion concentrations and Na + /K + -ATPase activities...
Includes: Supplementary data