...Junya Hiroi; Shigeki Yasumasu; Stephen D. McCormick; Pung-Pung Hwang; Toyoji Kaneko SUMMARY Cation–chloride cotransporters, such as the Na + /K + /2Cl – cotransporter (NKCC) and Na + /Cl – cotransporter (NCC), are localized to the apical or basolateral plasma membranes of epithelial cells...

...Junya Hiroi; Stephen D. McCormick SUMMARY We compared seawater tolerance, gill Na + /K + -ATPase and Na + /K + /2Cl – cotransporter (NKCC)abundance, and mitochondria-rich cell (MRC) morphology of three salmonids,lake trout Salvelinus namaycush , brook trout Salvelinus fontinalis and Atlantic salmon...

...Catherine Lorin-Nebel; Viviane Boulo; Charlotte Bodinier; Guy Charmantier SUMMARY This study combines a cellular and molecular analysis of the Na + /K + /2Cl - cotransporter (NKCC) to determine the osmoregulatory role of this protein in different tissues during the ontogeny of the sea bass. We have...

... under all conditions. By contrast, the expression of Na + /K + -ATPaseα-subunit mRNA and Na + /K + /2Cl - cotransporter mRNA was strongly responsive to external salinity. During acclimation to dilute seawater, cotransporter mRNA increased 10-20-fold in posterior gills within the first 24 h while Na + /K...

... absorption is proposed based on type-II cells possessing apical NKCC. * Author for correspondence (e-mail: [email protected] ) 16 3 2005 © The Company of Biologists Limited 2005 2005 mitochondrion-rich cell chloride cell Na + /K + -ATPase Na + /K + /2Cl - cotransporter...

... and electrophysiological studies indicate that a bumetanide-sensitive Na + /K + /2Cl – cotransporter is the primary route for basolateral ion entry into the cell during fluid secretion. The goal of this study was to resolve the apparent conflict between relatively high secretion rates by tubules bathed in K + -free saline...

...Stephen D. McCormick; Kristina Sundell; Björn Thrandur Björnsson; Christopher L. Brown; Junya Hiroi SUMMARY Na + /K + -ATPase,Na + /K + /2Cl - cotransporter (NKCC) and cystic fibrosis transmembrane conductance regulator (CFTR) are the three major transport proteins thought to be involved...

... of the putative electroneutral Na + /K + /2Cl - cotransporter by 10 μmol l -1 bumetanide reversibly decreased fluid secretion rates, with no detectable change in membrane potentials. Ouabain (1 mmol l -1 ), an Na + /K + -ATPase inhibitor, irreversibly decreased fluid secretion rates but had no effect...

... in the cytoplasm and basolateral membranes of all enterocytes and in the brush-border membrane of some cells, whereas NKCC immunofluorescence, demonstrating the presence of the Na + /K + /2Cl – cotransporter, was present in the cytoplasm and brush-border membrane. We conclude that the teleost intestine is capable...