... as ‘settlement’) are complex processes mediated by many signalling pathways. Nitric oxide (NO) signalling is one of the pathways that inhibits larval settlement in marine invertebrates across different phyla. NO is synthesized by NO synthase (NOS), which is a client of the molecular chaperone heat shock protein...

... of seawater killifish using an Ussing chamber approach. Pharmacological agents were used to explore the mechanism(s) triggering NO action. A modified Biotin-switch technique was used to investigate S -nitrosation of proteins. Stimulation of endogenous NO production via the nitric oxide synthase (NOS...

... ). The effect of 45 μmol l –1 N -acetylpenicillamine (NAP;Sigma, Israel), which lacks the NO donating S -nitroso group, was also examined. ELH (Peninsula Laboratories, Belmont, CA, USA) was prepared to reach a concentration of 0.15 μmol l –1 within the animal. The NOS (nitric oxide synthase) inhibitor N ω...