..., compared with the ionic composition of the surrounding seawater. We pharmacologically investigated the role of Na + /H + exchangers (NHE) in intracellular pH regulation and midgut proton and sodium maintenance using the NHE inhibitor 5-( n -ethyl- n -isopropyl)amiloride (EIPA). Basolateral EIPA application...

... ( Clark, 2004 ). Acidified water coupled with ion-poor conditions can be particularly challenging, as the excretion of acid–base equivalents is coupled to the uptake of certain ions that are limited ( Vangenechten et al., 1989 ). Ammonium transporter Carbon dioxide Disease vector NHE Rhesus...

... + /NH 4 + exchange by the gills, but not the yolk sac skin, of early­ life stage rainbow trout, possibly resulting from the lack of the key transporter NHE2 in the yolk sac epithelium. Larval fish PNA + ionocyte Na + /H + -exchanger NHE Rhesus glycoproteins Ion regulation...

... Calliphora vicina salivary gland intracellular pH regulation Na + /H + antiporter NHE vacuolar H + -ATPase V-ATPase intracellular pH insect blowfly BCECF NH 4 Cl prepulse The tubular salivary glands in the blowfly Calliphora vicina consist of a single layer of epithelial cells...

... to take up Na + across the apical membrane whereas C. v. variegatus relied on an NHE. Experiments with pharmacological inhibitors were designed to test this hypothesis and identify the most likely transport proteins involved in Na + uptake with the fewest experiments possible (i.e. paired experiments...

...Stefanos Dailianis; Efterpi Patetsini; Martha Kaloyianni SUMMARY This study investigated the role of Na + /H + exchanger(NHE) and signalling molecules, such as cAMP, PKC, PI 3-kinase, and immune defence enzymes, NADPH oxidase and nitric oxide synthase, in the induction of protein glutathionylation...

... binding and translocation. Based on the crystal structure of NhaA, a model structure of the human Na + /H + exchanger (NHE1) was constructed, paving the way to a rational drug design. * Author for correspondence (e-mail: [email protected] ) 19 11 2008 2009 NhaA NHA NHE Na...

... on NHE expression were investigated. The elevation of plasma cortisol using intraperitoneal implants caused a significant increase in NHE2 mRNA expression without affecting NHE3 mRNA or protein abundance. Thus, we suggest that NHE2 contributes to acid–base regulation during hypercapnia owing to its...

...; it provides weak HCO 3 – and weaker CO 3 2– anions to the lumen. The cationic pathway consists of H + V-ATPases and Na + /H + antiporters (NHAs), Na + /K + P-ATPases and Na + /H + exchangers (NHEs) along with several(Na + or K + ):amino acid +/– symporters,a.k.a. nutrient amino acid transporters (NATs...

... concanamycin A; (7) concanamycin A removes alkali-going components from multiphasic 5-HT-induced pH changes; (8) NHE activity and a Cl – -dependent process are involved in generating 5-HT-induced pH changes; (9) the salivary glands probably contain a Na + -driven amino acid transporter. We conclude that V...

... of British Columbia Okanagan, Kelowna, BC, V1V 1V7, Canada 20 8 2007 © The Company of Biologists Limited 2007 2007 sodium potassium exchanger alkalinization AgNHA NHA AgNHE NHE CHA CHE CPA2 African malaria mosquito All organisms regulate their cellular pH, volume...

.... Hyperosmotic shrinkage by a calculated 25% activated a robust Na influx that in the first 30 min had a strong P O 2 dependency with maximal activation at low P O 2 values and a P 50 of ∼5.5 kPa. This activation was completely abolished by the Na/H exchanger (NHE) inhibitor EIPA (10 –4 mol l -1 ). Hyperosmotic...

... but contribute both to H + extrusion after an acute dopamine- or NH 4 Cl-induced acid load. vacuolar H + -ATPase V-ATPase NHE BCECF intracellular pH dopamine biogenic amines insect cockroach Periplaneta americana salivary glands References Baumann, O., Dames, P., Kühnel, D. and Walz...

....,Mackenzie, L. and Morrison-Shetlar, A. ( 1999 ). A mechanism for branchial acid excretion in marine fish: identification of multiple Na + /H + antiporter (NHE) isoforms in gills of two seawater teleosts. J. Exp. Biol. 202 , 315 -324. Claiborne, J. B., Edwards, S. L. and Morrison-Shetlar, A. I...

...-potential-sensitive, Na + -accepting ion channel was also demonstrated. The basolateral Na + /H + exchanger physiologically resembles members of the NHE family of Na + /H + antiporters described in vertebrates and departs from the apical electrogenic system previously described in lobster. Whether...

... in marine invertebrates . Comp. Biochem. Physiol . 404C , 355 – 372 . Wakabayashi , S. , Sardet , C. , Farfournoux , P. , Counillon , L. , Meloche , S. , Pages , G. and Pouyssegur , J. ( 1992 ). Structure–function of the growth factor-activatable Na/H exchanger (NHE...