... in a competitive manner with ammonia. In situ hybridization experiments showed that both transporters were expressed as early as the 4-cell stage in zebrafish embryos and could be identified in most tissues 4 days post-fertilization. Larvae experiencing morpholino-mediated knockdown of DrHiat1b exhibited...

... . Climate change Embryonic development Energy metabolism Invasive species Morpholino The Company of Biologists http://dx.doi.org/10.13039/501100000522 JEBTF-151217 Centre National de la Recherche Scientifique http://dx.doi.org/10.13039/501100004794 Sorbonne University...

...Alex M. Zimmer; Yihang K. Pan; Theanuga Chandrapalan; Raymond W. M. Kwong; Steve F. Perry ABSTRACT Loss-of-function technologies, such as morpholino- and RNAi-mediated gene knockdown, and TALEN- and CRISPR/Cas9-mediated gene knockout, are widely used to investigate gene function and its...

... neurotransmitter, plays a modulatory role in controlling breathing in zebrafish during acute changes in environmental oxygen levels, and its role changes throughout development. Chemoreceptor Hypoxia Hyperoxia Immunohistochemistry Neuroepithelial cell Morpholino Gasotransmitters are endogenous...

... β1AR gene and two β2AR genes (β 2aAR and β 2bAR ). We examined the roles of these receptors in larval zebrafish in vivo by assessing the impact of translational gene knockdown on cardiac function. Zebrafish larvae lacking β1AR expression by morpholino knockdown displayed lower heart rates than control...

... shortly after birth; however, a cardiac cause is possible. The zebrafish is an ideal model to investigate the role of fxr1 during development of the heart. We have carried out morpholino knockdown of fxr1 and have demonstrated abnormalities of striated muscle development and abnormal development...