...Dennis Kolosov; Sima Jonusaite; Andrew Donini; Scott P. Kelly; Michael J. O'Donnell ABSTRACT The Malpighian tubules (MTs) and hindgut together act as the functional kidney in insects. MTs of caterpillars are notably complex and consist of several regions that display prominent differences in ion...

...), the coordinated activity of the Malpighian tubules and anterior midgut maintains water and ion balance during the post-prandial diuresis. Three to four hours after the meal, the diuretic process finishes, and the animal enters an antidiuretic state to ensure water conservation until the next blood intake...

.... Larval stage mosquitoes must deal with the potential dilution of their haemolymph in freshwater, whereas the haematophagus A. aegypti female faces the challenge of excess ion and water intake after a blood meal. The excretory system, composed of the Malpighian tubules (MTs) and hindgut, is strictly...

... quickly, and better maintained hemolymph K + balance in the cold. The improvements in chill tolerance coincided with increased Malpighian tubule fluid secretion and better maintenance of K + secretion rates in the cold, as well as reduced rectal K + reabsorption in cold-acclimated flies. To test whether...

... cofactors, signalling, storage or excretion. Here we identify zinc storage granules as the insect's major zinc reservoir in principal Malpighian tubule epithelial cells of Drosophila melanogaster . The concerted action of Adaptor Protein-3, Rab32, HOPS and BLOC complexes as well as of the white-scarlet...

... loads largely outside the ECF, in the lumen of the gut. This strategy may be related to the apparent absence of Malpighian tubules in aphids. Other insects can suspend ECF homeostasis altogether in order to survive extreme temperatures. Thus, ECF homeostasis in insects is highly dynamic and plastic...

...Alan T. Marshall; Peta L. Clode SUMMARY Elemental imaging by X-ray microanalysis of fully frozen-hydrated samples shows that when Malpighian tubules of the black field cricket( Teleogryllus oceanicus L.) are incubated in saline in which Rb + has been substituted for K + , Rb + replaces much...

... and by Malpighian tubules (MTs) in Drosophila. The kidney serves as an excellent model organ to investigate the cellular and molecular mechanisms underlying organogenesis. Mammals and Drosophila share common principles of renal development. Tissue homeostasis, which is accomplished through self-renewal...

...Andrew Donini; Michael J. O'Donnell; Ian Orchard SUMMARY The effects of corticotropin-releasing factor (CRF)-related (ZooneDH),calcitonin (CT)-related (RhoprDH 31 ) and kinin-related (leucokinin I) peptides on the ion composition of fluid secreted by upper Rhodnius prolixus Malpighian tubules...

... body water was elevated during acute exposure to acidic media. During chronic exposure, total body water was decreased and Malpighian tubule mitochondrial luminosity, quantified using Mitotracker Green FM, increased. Malpighian tubule secretion rates and energy demands thus appear to increase...

...Maria Soledad Santini; Jorge Rafael Ronderos SUMMARY Haematophagous insects incorporate a large amount of blood with each meal,producing a big quantity of urine in a few hours to eliminate the excess water and Na + . Malpighian tubules (MTs) have traditionally been seen as a system that responds...

...Douglas S. G. Neufeld; Ross Kauffman; Zachary Kurtz SUMMARY We demonstrate the presence of an efficient, multispecific transport system for excretion of organic anions in the Malpighian tubules of the cricket Acheta domesticus using fluorescein (FL) as a model substrate. Malpighian tubules rapidly...

...Klaus W. Beyenbach SUMMARY We have studied Malpighian tubules of Aedes aegypti using a variety of methods: Ramsay fluid secretion assay, electron probe analysis of secreted fluid, in vitro microperfusion and two-electrode voltage clamp. Collectively, these methods have allowed us to elucidate...

...U. I. M. Wiehart; G. Klein; P. Steels; S. W. Nicolson; E. Van Kerkhove SUMMARY The presence of ATP-regulated K + (K ATP ) channels in Tenebrio molitor Malpighian tubules was investigated by examining the effect of glibenclamide on both fluid secretion and basolateral membrane potentials ( V bl...

...U. I. M. Wiehart; S. W. Nicolson; E. Van Kerkhove SUMMARY Malpighian tubules of the mealworm Tenebrio molitor were isolated for intracellular measurement of basolateral ( V bl ) and,indirectly, apical ( V ap ) membrane potentials. In control Ringer (50 mmol l -1 K + , 140 mmol l -1 Na + ), V bl...

...Douglas S. Neufeld; John P. Leader ABSTRACT The ability of isolated Malpighian tubules from a freeze-tolerant insect, the New Zealand alpine weta ( Hemideina maori ), to withstand freezing was assessed by measuring post-freeze membrane potentials and rates of fluid secretion. The hemolymph of cold...

...Leon C. Isaacson; Susan W. Nicolson ABSTRACT Using cyclic AMP to stimulate perfused tsetse fly Malpighian tubules bathed in SO 4 2− Ringer frequently causes an immediate but transient peak in transtubular potential ( V t ), before stabilisation of V t at an increased value. These transients were...

...Michael J. O’Donnell; Simon H. P. Maddrell ABSTRACT The properties of the Malpighian tubules of Drosophila melanogaster change along their length. The upstream main segments secrete K + -rich fluid at a high rate. From this, the lower tubules reabsorb significant amounts of water and K + . Under...

...; the osmolality of the solution was 375 mosmol kg −1 H 2 O). Solutions were freshly prepared each week, filtered through 0.45 μ m Millipore filters and kept at 2°C until use. 7 6 1994 ©The Company of Biologists Limited 1994 Malpighian tubules K + uptake mechanisms fluid secretion...

...Geoffrey M. Coast; Iain Kay ABSTRACT Acheta diuretic peptide ( Acheta -DP) is a corticotropin-releasing factor (CRF)-related peptide found in head extracts of the house cricket Acheta domesticus . The peptide causes a dose-dependent increase in fluid secretion by cricket Malpighian tubules isolated...