... showed that adrenaline elicits a dose-dependent β-adrenoreceptor (AR)-mediated increase in f V that does not require expression of β1-ARs, as the hyperventilatory response to β-AR stimulation was unaltered in adrb1 −/− mutants, generated by CRISPR/Cas9 knockout. In response to hypoxia and propranolol co...

... of knockout of Sap47 or synapsin on olfactory associative learning/memory support the hypothesis that both proteins operate in the same molecular pathway. We therefore asked if this might also be true for other aspects of their function. We show that knockout of Sap47 but not synapsin reduces lifespan...

...Alex M. Zimmer; Yihang K. Pan; Theanuga Chandrapalan; Raymond W. M. Kwong; Steve F. Perry ABSTRACT Loss-of-function technologies, such as morpholino- and RNAi-mediated gene knockdown, and TALEN- and CRISPR/Cas9-mediated gene knockout, are widely used to investigate gene function and its...