... paracellular pathway and an increase in conductance of the epithelium. Tight junction CFTR Immunocytochemistry Electrophysiology Fundulus heteroclitus Euryhaline Teleost fish Gill epithelium Ionocyte Chloride cell The mummichog (or common killifish) Fundulus heteroclitus is well...

... labeling pattern was somewhat different from that revealed in the RT-PCR experiments on the distribution of mRNA in the tissues. In the RT-PCR analyses, AQP-x5 mRNA was detected in the brain, lungs and testes,which is contrary to the immunocytochemistry results that showed no immunopositive cells...

... cardiac ganglion neuron neurotransmitter stomatopod Squilla oratoria immunocytochemistry GABA glutamate Alexandrowicz (1934) first showed that three pairs of central cardioregulatory nerves (α, βand γ) control the heart of the stomatopod Squilla mantis . Of these, β and γ are most...

... heart Antarctic teleost icefish Chionodraco hamatus myocardial performance nitric oxide synthase (NOS) immunocytochemistry Over the past years the universal signaling molecule nitric oxide (NO) has revealed itself to be a major modulator of cardio-circulatory function in mammals (Shah...

... 2004 © The Company of Biologists Limited 2004 2004 serotonin 5-hydroxytryptamine dopamine synapsin innervation synapse immunocytochemistry salivary gland insect cockroach Periplaneta americana The salivary glands in the cockroach Periplaneta americana are of the acinar...

... + -ATPase serotonin 5-hydroxytryptamine regulation assembly immunocytochemistry insect blowfly Calliphora vicina . The salivary glands of the blowfly are thin tubules that extend as a pair into the abdomen of the animal. The abdominal portions of the gland are secretory and produce a K...

..., was not dependent on the presence of external Ca 2+ or Na + . Finally, the teleost Ca 2+ -sensing receptor (Ca-SR) was shown to be highly expressed in a subpopulation of olfactory receptor neurones by both immunocytochemistry and in situ hybridisation. The olfactory sensitivity to Ca 2+ (and Mg 2+) is therefore...

... (O’Grady, 1989) and in goldfish (Bakker et al., 1993) intestine. ANP and VIP were hypothesized to be released by enteric nerves and to act locally (O’Grady, 1989), and this localization has been definitively demonstrated by fluorescence immunocytochemistry (Loretz et al., 1997). The function...

... significantly with regard to their biological effects. Semi-isolated crayfish hearts and locust oviducts did not respond to the three peptides. Immunocytochemistry using antisera against Asn 13 - orcokinin and orcomyotropin showed that these neuropeptides are co-localized in approximately 80–90 neurones...

... of these findings within the broad context of vertebrate extraretinal photoreception. * Author for correspondence (e-mail: [email protected]) 30 03 2000 23 05 2000 © 2000 by Company of Biologists 2000 extraretinal photoreceptor teleost fish immunocytochemistry in situ...

...-mail: [email protected] 17 02 2000 06 04 2000 © 2000 by Company of Biologists 2000 GABA γ-aminobutyric acid immunocytochemistry growth hormone oestradiol goldfish Carassius auratus Growth is seasonally regulated in goldfish, and the highest growth rates...

... labelled (Fig. 4D). * e-mail: [email protected] 01 6 1998 27 7 1998 © The Company of Biologists Limited 1998 temporary adhesion podium footprint ultrastructure biochemical composition immunocytochemistry Echinodermata Asteroidea Asterias rubens...

...Ann Jane Tierney; Jennifer Blanck; A. Joffre Mercier ABSTRACT Whole-mount immunocytochemistry was used to map the location of FMRFamide-like peptides in the crayfish (Procambarus clarkii) stomatogastric nervous system. This system contains the pyloric and gastric mill central pattern generators...

...The Company of Biologists Limited 1997 epididymis vas deferens pH regulation immunocytochemistry bafilomycin Many transporting epithelia contain a subpopulation of specialized cells that contain high levels of a vacuolar-type proton-pumping H + ATPase (V-ATPase) on their plasma membrane...

.... Digoxigenin-labelled RNA probes were used for in situ hybridization of V-ATPase mRNA of both peripheral and integrated subunits; monoclonal antibodies to subunits of the peripheral sector of the purified plasma membrane V-ATPase were used for immunocytochemistry. Extensive mRNA labelling was found in both...

... in all assayed tissues and that additional, hitherto uncharacterised, forms of tachykinin-immunoreactive material may be present in the body ganglia and midgut as well as in the larval CNS. An antiserum was raised to CavTK-II for immunocytochemistry. This antiserum, which was found to be specific...

...Gary V. Sullivan; James N. Fryer; Steve F. Perry ABSTRACT The expression of the V-type proton ATPase (H + -ATPase) was examined in the gill of the freshwater rainbow trout (Oncorhynchus mykiss) using immunocytochemistry in concert with laser scanning confocal or electron microscopy. A synthetic...

... N terminus containing multiple potential phosphorylation sites. Western analysis and immunocytochemistry in frozen sections of mouse brain demonstrated a clear caudal–rostral gradient of GLYT2 distribution, with massive accumulation in the spinal cord and brainstem and less in the cerebellum. Its...

... with permission from Lorang et al . (1994) . The distribution of NET-mRNA-expressing neurons in the rat CNS has recently been reported by Lorang et al . (1994) . In these studies, NET mRNA was localized by in situ hybridization performed in parallel with immunocytochemistry of tyrosine hydroxylase...

... 1994 neuropeptides neuromodulators confocal microscopy immunocytochemistry crustaceans Cancer borealis crab The myomodulin family consists of nine related peptides (myomodulins A–I), all possessing a common Met-Leu-Arg-Leu-NH2 carboxyl terminal sequence (Cropper et al . 1987...