...Alex M. Zimmer; Khatereh Shir-Mohammadi; Raymond W. M. Kwong; Steve F. Perry ABSTRACT Freshwater fishes absorb Na + from their dilute environment using ion-transporting cells. In larval zebrafish ( Danio rerio ), Na + uptake is coordinated by (1) Na + /H + exchanger 3b (Nhe3b) and (2) H + -ATPase...

... H + -ATPase (VHA)-assisted uptake via a Na + channel and a NHE-based mechanisms for Na + uptake. Immunolocalization of VHA and Na + /K + -ATPase (NKA) indicated at least two cell types present within leech skin, VHA + and VHA − cells, where the VHA + cells are probably involved in Na + uptake. NKA...

... control levels over time. Gill H + -ATPase activity increased by more than 45% 14 days after exposure to freshwater, whereas H + -ATPase mRNA levels were not affected by the salinity change. Within 4 days of freshwater exposure, gill Na + /K + -ATPase activity increased ∼43% over control levels, remaining...

... environmental ammonia (HEA; 1 and 5 mmol l –1 NH 4 HCO 3 ) and evaluated the patterns of ammonia excretion and gill Rh mRNA and protein expression. Gill H + -ATPase , NHE1 , NHE2 , NHE3 , Na + /K + -ATPase ( NKA ), Na + /K + /2Cl – co-transporter ( NKCC1 ) mRNA, H + -ATPase activity, NKA protein and activity...

... transporters (Rhcg, V-type H + -ATPase, Na + /H + exchanger NHE-2 and/or NHE-3, Na + channel) working together as a metabolon provides an acid trapping mechanism for apical excretion. Intracellular carbonic anhydrase(CA-2) and basolateral Na + /HCO – 3 cotransporter (NBC-1) and Na + /K + -ATPase play indirect...

... Physiol. 280 , F426 -F436. Beaulieu, V., Da Silva, N., Pastor-Soler, N., Brown, C. R.,Smith, P. J., Brown, D. and Breton, S. ( 2005 ). Modulation of the actin cytoskeleton via gelsolin regulates vacuolar H + ATPase (V-ATPase) recycling. J. Biol. Chem. 280 , 8452 -8463. Beyenbach, K. W...

... ) 26 1 2009 © The Company of Biologists Limited 2009 2009 H + -ATPase soluble adenylyl cyclase intercalated cells proton secretion acidification pH sensing The V-ATPase is a ubiquitous enzyme that is best known for its role in acidifying various intracellular organelles...

... of Rhbg, Rhcg2,H + -ATPase and Na + /H + exchanger-2 (NHE-2) were up-regulated in DSI pre-exposed to ammonia and cortisol, CA-2 mRNA was down-regulated, and transepithelial potential became more negative. Bafilomycin (1 μmol l –1 ), phenamil (10 μmol l –1 ) and 5-( N,N -hexamethylene)amiloride (HMA...

...Martin Tresguerres; Scott K. Parks; Fumi Katoh; Greg G. Goss SUMMARY We have previously shown that continuous intravenous infusion of NaHCO 3 for 24 h (∼1000 μmol kg -1 h -1 )results in the relocation of V-H + -ATPase from the cytoplasm to the basolateral membrane in the gills of the Pacific...

... proton ATPase (V-H + -ATPase)-rich or sodium-potassium ATPase(Na + /K + -ATPase)-rich cells in all fish examined. A minority of the cells also labeled positive for both transporters. Gill cell membranes prepared from NaHCO 3 -infused fish showed significant increases in both V-H + -ATPase abundance (300...

... + /K + -ATPase activity increased fivefold and H + -ATPase activity decreased fourfold when trout were acclimated to either 10‰ or 30‰ seawater. When total MR gill cells were separated based on differential binding to peanut lectin agglutinin (PNA), the PNA subtypes underwent a change in relative...

... was partially inhibited by bafilomycin, indicating the possible involvement of a V-type H + -ATPase. P. schlosseri could also increase the rate of H + excretion in response to the presence of ammonia in a neutral (pH 7.0) external medium. Increased H + excretion in P. schlosseri occurred in the head region...

..., NJ 08544, USA (e-mail: [email protected] ) 20 5 2002 © The Company of Biologists Limited 2002 2002 oxygen pH ammonia H + -ATPase reaction-diffusion Dictyostelium discoideum In 1995, Bonner and his colleagues( Bonner et al., 1995 ) reported a rapid...

... turnover pHe H + -ATPase pHi metabolic rate Na + /H + exchange Na + /K + -ATPase Na + -dependent Cl − /HCO 3 − exchange 31 P-NMR transport energetics Sipunculus nudus The maintenance of ion gradients over cell membranes constitutes an important fraction of cellular energy...

... epithelium, Na + /H + exchangers (both NHE2- and NHE3-like isoforms), a cystic fibrosis transmembrane regulator (CFTR)-like anion channel, a vacuolar-type H + -ATPase (V-ATPase) and carbonic anhydrase immunoreactivity are associated with the apical crypt region of MR cells. Associated with the MR cell...

..., Rua do Campo Alegre 823, 4150-180 Porto, Portugal (e-mail: [email protected] ) 03 05 2000 10 07 2000 © 2000 by Company of Biologists 2000 fish gill ion transport H + -ATPase Na + /K + -ATPase epithelial Na + channel Na + /H + exchanger anion exchanger pavement...

... uptake of Na + by freshwater fish. * e-mail: [email protected] 27 9 1999 29 11 1999 © 1999 by Company of Biologists 1999 H + -ATPase V-type ATPase proton pump Na + Cl − teleost bafilomycin A1 gill tilapia Oreochromis mossambicus carp Cyprinus...

... − current densities. As previously observed in NG108-15 cells, the vacuolar-type H + -ATPase inhibitors bafilomycin A1 and nitrate induced membrane depolarizations in NG/DOXR cells. The resting potentials of sensitive and resistant cells were not significantly different, but the depolarizations evoked...

...Gary V. Sullivan; James N. Fryer; Steve F. Perry ABSTRACT The expression of the V-type proton ATPase (H + -ATPase) was examined in the gill of the freshwater rainbow trout ( Oncorhynchus mykiss ) using immunocytochemistry in concert with laser scanning confocal or electron microscopy. A synthetic...

... cells. For the first time, the Na + /K + -ATPase and H + -ATPase activities of fish gills were determined in intact cells in situ . The activity of both ATPases was dependent on alamethicin concentration (optimum 100 μ g mg −1 protein) and on preincubation time (optimum 10 min). The in situ activity...