... fura-2 mitochondria Most mammalian neurons have a low tolerance to severe arterial hypoxia resulting in brain anoxia ( Hansen,1985 ; Haddad and Jiang,1993 ). In many brain regions, this is due to the fact that anoxia-evoked extracellular accumulation of glutamate is excitotoxic( Choi, 1994...

... of rainbow trout ( Oncorhynchus mykiss ) were measured. Cell shortening, expressed as a percentage of resting cell length, was our index of contractility. The fluorescent Ca 2+ indicator Fura-2 was used to monitor changes in [Ca 2+ ] i . Action potentials and L-type Ca 2+ currents (I Ca ) were recorded using...

...L. Potvin; R. Laprade; J. L. Schwartz ABSTRACT Intracellular Ca 2+ concentration was measured in single Cf1 cells ( Choristoneura fumiferana , spruce budworm) loaded with Fura-2, a Ca 2+ -sensitive fluorescent probe. Cf1 cells displayed Ca 2+ surges in response to Cry1Ac and Cry1C proteins, two Cf1...

...) and furosemide. Chelation of either extracellular (1 mmol l −1 EGTA) or both extra- and intracellular Ca 2+ (1 mmol l −1 EGTA, 10 µmol l −1 A23187) had no effect on this RVD process. Furthermore, as measured using the fluorescent dye Fura-2/AM, there was no increase in the intracellular free Ca 2+ concentration...

...+ indicators Fluo-3 and Fura-2. Responses of lymphocytes to T-cell mitogen (phytohaemagglutinin, PHA), to B-cell mitogen (lipopolysaccharide, LPS) and to immunoglobulin (Ig) cross-linking with a monoclonal antibody to carp Ig were measured using flow cytometry. Both T-cell stimulation by PHA and B-cell...

... velocity, measured using Fura-2 at room temperature (approximately 22°C), was highest in cold-acclimated trout, and the values for adult rat, warm-acclimated trout, newborn rat, warm-acclimated carp and cold-acclimated carp were 93, 56, 24, 21 and 14% of the uptake velocity of cold-acclimated trout...

...Petra Wetzel; Gerolf Gros ABSTRACT Isometric single-twitch force and intracellular Ca 2+ transients were recorded simultaneously, using fura-2, from slow- and fast-twitch muscle fibres of the rat, mouse and Etruscan shrew Suncus etruscus . In the slow-twitch rat soleus, force half-relaxation time...

... of the leech Hirudo medicinalis . Exposed glial cells of isolated ganglia were filled iontophoretically with the Ca 2+ indicator dye Fura-2. Application of glutamate (200–500 μmol l −1 ) caused biphasic membrane potential shifts and increases in [Ca 2+ ] i , which were only partly reduced by either removing...

...-aspartate (NMDA) receptors, we measured the effect of adenosine on NMDA-mediated Ca 2+ transients in normoxic and anoxic turtle cerebrocortical sheets. Intracellular [Ca 2+ ] was measured fluorometrically with the Ca 2+ -sensitive dye Fura-2. Baseline intracellular [Ca 2+ ] and [ATP] were also measured...

... of the fluorescent Ca 2+ indicator Fura-2. Digitized fluorescence microscopy of the Fura-2-loaded cells enabled measurement of intracellular Ca 2+ levels in individual cells. Intracellular free Ca 2+ levels were measured in CA cells from selected days during the fifth larval stadium and were found to be highest when...

... simultaneously in anterior pituitary cells from adult male rats. This was made possible using a combination of digital imaging video microscopy with the fluorescent calcium indicator Fura-2 and the reverse haemolytic plaque assay (RHPA) to identify the cell type and measure hormone secretion from the cells under...

... interaction that generates synergy. Research emphasizes the single cell approach. Direct measurements of intracellular free Ca 2+ were performed using the Ca 2+ -sensitive fluorescent probe fura-2/AM and instrumentation for digital image processing. A reverse haemolytic plaque assay was used to measure...