... paracellular pathway and an increase in conductance of the epithelium. Tight junction CFTR Immunocytochemistry Electrophysiology Fundulus heteroclitus Euryhaline Teleost fish Gill epithelium Ionocyte Chloride cell The mummichog (or common killifish) Fundulus heteroclitus is well...

... chloride cell osmorespiratory compromise paracellular permeability ionic regulation Na + /K + -ATPase The fish gill (Evans et al.,2005) is the site of numerous physiological processes including chemoreception (Burleson et al.,1992 ; Burleson,1995 ; Jonz et al.,2004 ; Burleson et al.,2006...

... and SW salmon. Labelling occurred mainly in presumed chloride cells and cells deep in the filament but occasionally also on lamellae. Overall, the salinity-induced variation in labelling pattern and intensity matched the quantification data. In conclusion, the predominant switching of Na + ,K + –ATPase α...

... 6 2008 © The Company of Biologists Limited 2008 2008 cation–chloride cotransporter Na + /K + /2Cl – cotransporter Na + /Cl – cotransporter ion transport mitochondria-rich cell chloride cell tilapia Euryhaline teleost fish, such as Mozambique tilapia, Oreochromis...

... location have not been identified with certainty. Mitochondria-rich cell chloride cell peanut lectin agglutinin hypercapnia cortisol gill NHE NHE2 NHE3 sodium/proton exchangers fish © The Company of Biologists Limited 2008 2008 31 3 2008 * Author for correspondence (e...

...-mail: [email protected]) 31 3 2008 © The Company of Biologists Limited 2008 2008 chloride cell organic osmolyte osmosensor pavement cell Ostf transcription factor The capability of animal cells to maintain a constant cell volume is an essential prerequisite for cellular life...

...-1 Sugao, Miyamae-ku, Kawasaki 216-8511, Japan (e-mail: [email protected]) 17 1 2007 © The Company of Biologists Limited 2007 2007 mitochondria-rich cell chloride cell salinity tolerance Na + /K + -ATPase Na + /K + /2Cl – cotransporter salmonid lake trout brook...

... + -ATPase, which are essential for ionic regulation in seawater, and the appearance of chloride cell-accessory cell complexes,which are normally restricted to fish inhabiting seawater. These data provide compelling evidence that gill remodelling during migration from freshwater to seawater may involve...

... that an ancestral form of the mammalian genes diverged from those in the lower vertebrates prior to the gene duplication event that gave rise to TRPV5 and TRPV6. The putative model for Ca 2+ uptake in fish proposes that the mitochondria-rich cell (also termed ionocyte or chloride cell) is the predominant...

... in the gills (chloride cells) rapidly regulate NaCl secretion for systemic osmoregulation while they simultaneously are exposed to acute osmotic shock. This study was conducted to determine if COX2 is expressed in the gills, and if so, to evaluate its function in cellular and systemic osmoregulation...

.... and Marshall, W. S. (2001). Dynamics of pavement cell-chloride cell interactions during abrupt salinity change in Fundulus heteroclitus . J. Exp. Biol. 204 , 1889 -1899. Evans, D. H., Piermarini, P. M. and Choe, K. P. (2005). The multifunctional fish gill: dominant site of gas exchange, osmoregulation...

...) cell, intercalated MR cell, chloride cell and pavement cell] differ in several respects from those of teleosts. The ultrastructural characteristics of these cells are distinctive and closely resemble those of certain ion-transporting epithelia in other vertebrates, for which the function has been...

...Raquel N. Lima; Dietmar Kültz SUMMARY The effects of salinity on chloride cells (CC) and Na + /K + -ATPase content in gill epithelium of euryhaline killifish Fundulus heteroclitus were analyzed using laser scanning cytometry (LSC) and tissue microarrays (TMAs). Salinity acclimations consisted...

... consumption, drinking rate, hematocrit, mean cell hemoglobin concentration, and muscle water content), biochemical(Na + , K + -ATPase) and morphological (number of mature,accessory, immature and apoptotic chloride cells) indicators of osmoregulatory stress were measured. Tilapia tolerated salinities ranging...

... of Biologists Limited 2003 2003 temperature acclimation osmoregulation Na + /K + -ATPase cortisol prolactin chloride cell immunohistochemistry real-time RT-PCR common carp Cyprinus carpio The common carp Cyprinus carpio L. is a stenohaline freshwater species. Carp originated...

...Katherine A. Sloman; Patrick R. Desforges; Kathleen M. Gilmour SUMMARY Fish acclimated to ion-deficient water exhibit proliferation of branchial chloride cells. The objective of the present study was to investigate the role of cortisol in this response using the corticosteroid receptor antagonists...

... euryhaline fish gill epithelium salinity adaptation chloride cell killifish Fundulus heteroclitus . Euryhaline teleosts maintain their plasma osmotic homeostasis by extrarenal transport of salt. In a plasma-hyperosmotic environment (e.g. sea water, SW) they actively secrete NaCl, while...

...Kiyono Shiraishi; Junya Hiroi; Toyoji Kaneko; Manabu Matsuda; Tetsuya Hirano; Takao Mori SUMMARY To examine the functional differentiation of chloride cells in the yolk-sac membrane of tilapia (Oreochromis mossambicus) embryos, we developed a ‘yolk-ball’ incubation system in which the yolk sac...

...Zhichao Dang; Paul H. M. Balm; Gert Flik; Sjoerd E. Wendelaar Bonga; Robert A. C. Lock ABSTRACT The effect of cortisol on Na + /K + -ATPase expression in the gill chloride cells of tilapia Oreochromis mossambicus was studied by immunocytochemistry at the light and electron microscope levels. One...

... and mitochondria-rich (MR) cells (15.7±2.5 % of total cell numbers). MR cells occur singly or in clusters, are voluminous, open apically to the ‘external environment’ and exhibit ultrastructural characteristics similar to those found in the ‘chloride cells’ of freshwater fish gills. After 6–9 days in culture...