... technological innovations like next-generation sequencing and CRISPR-Cas9 genome editing, the time is ripe to move beyond the big 6. In this review, we chart a 10-step path to this goal, using our own experience with the Aedes aegypti mosquito, which we built into a model organism for neurobiology in one decade...

... reproduction rather than to improve tolerance during cold exposure. Genome editing CRISPR-Cas9 Cold tolerance Chill coma Intrinsically disordered protein Insect In temperate, polar and alpine environments, insects and other small ectotherms risk internal ice formation and death when exposed...