... or enhancer that drives expression of the wing selector gene, vestigial ( vg ), whose function is broadly conserved in insects and contributes to wing size. To test this hypothesis directly, we used CRISPR/Cas9 to replace the DNA sequence of the predicted Quadrant Enhancer ( vg QE ) from D. virilis...

... in the developing gut. Using optimized CRISPR/Cas9 gene editing methods to achieve high editing efficiency in the F 0 generation, we generated ABCB1a crispant embryos with significantly reduced transporter efflux activity. When infected with the opportunistic pathogen Vibrio diazotrophicus , Sp-ABCB1a crispant...

... and pituitary cells immunoreactive to Sl was increased in individuals reared with black backgrounds compared with that in fish grown in white tanks. In larval stages of the medaka Oryzias latipes , a similar response was observed, which was altered by ghr1 biallelic mutations using CRISPR/Cas9 . Interestingly...

... related to the nature of our earliest vertebrate ancestors. Here, we describe how genetic analysis of embryogenesis in the sea lamprey ( Petromyzon marinus ) has provided insight into the origin and evolution of developmental-genetic programs in vertebrates. We focus on recent work involving CRISPR/Cas9...

...Virginie Courtier-Orgogozo; Arnaud Martin; Michael H. Dickinson; Leslie B. Vosshall; Julian A. T. Dow ABSTRACT One promising application of CRISPR/Cas9 is to create targeted mutations to introduce traits of interest into domesticated organisms. However, a major current limitation for crop...

...Alex M. Zimmer; Yihang K. Pan; Theanuga Chandrapalan; Raymond W. M. Kwong; Steve F. Perry ABSTRACT Loss-of-function technologies, such as morpholino- and RNAi-mediated gene knockdown, and TALEN- and CRISPR/Cas9-mediated gene knockout, are widely used to investigate gene function and its...