... of inhibitors of specific transporters to assess the mechanisms of Na + transport across the nuchal organ. Na + influx across the nuchal organ was inhibited both by inhibitors of the Na + /K + -ATPase (ouabain, bufalin) and by inhibitors of the vacuolar H + -ATPase (bafilomycin, N -ethylmaleimde, 7-chloro-4...

... + /K + -ATPase inhibitor ouabain (10 mmol l –1 ; 21% reduction) and the vacuolar H + -ATPase inhibitor bafilomycin (5 μmol l –1 ; 21% reduction), and by the organic anions PAH (10 mmol l –1 ; 44% reduction) and probenecid (10 mmol l –1 ; 61% reduction, concentration-dependent inhibition). Transport...

... inhibitor bafilomycin A 1 , which halts embryonic development. Importantly, net proton flux from V-ATPase-acidified compartments to the surrounding cytoplasm is likely under anoxia and may significantly contribute to the enigmatic acidification critical to quiescence. * Author for correspondence (e...

... specific inhibitors for these ion pumps, namely ouabain and bafilomycin A 1 . In control glands, bafilomycin-A 1 -sensitive V-ATPase activity and ouabain-sensitive Na + /K + -ATPase activity accounted for 36% and 19%, respectively, of the total ATPase activity. V-ATPase activity increased approximately...

.... Treatment with colchicine impairs the migration of V-ATPase-positive vesicles to the apical cell membrane and abates the acidification of the UVD lumen. Bafilomycin, a selective inhibitor of V-ATPase activity, also prevents the decline in luminal pH. We conclude that the circadian clock generates a rhythm...

... profiles, were observed in individuals of two Aedes species, Aedes aegypti from semi-tropical Florida and Aedes canadensis from north-temperate Massachusetts. The gradients remained in a steady state for up to 6 h, the maximum duration of the recordings. Bafilomycin A 1 (10 −5 , 10 −7 mol l −1...

...Klaus W. Beyenbach; Thomas L. Pannabecker; Wolfram Nagel ABSTRACT The effects of bafilomycin A 1 , a blocker of V-type H + -ATPases, were investigated in Malpighian tubules of Aedes aegypti . Bafilomycin A 1 reduced rates of transepithelial fluid secretion and the virtual short-circuit current ( vI...

...; in the anterior midgut (pH 10–11), they are located basally; and in the posterior midgut (pH≈8) they are again located apically. The hypothesis that the energization of alkalization is mediated by an H+ V-ATPase is supported by the inability of larvae to maintain the high pH after 72 h in 10 μM bafilomycin B1...

... − current densities. As previously observed in NG108-15 cells, the vacuolar-type H + -ATPase inhibitors bafilomycin A1 and nitrate induced membrane depolarizations in NG/DOXR cells. The resting potentials of sensitive and resistant cells were not significantly different, but the depolarizations evoked...

... Street, Charlestown, MA 02129, USA (e-mail: [email protected] ). ©The Company of Biologists Limited 1997 epididymis vas deferens pH regulation immunocytochemistry bafilomycin Many transporting epithelia contain a subpopulation of specialized cells that contain high...

... . 1983 ). * Present address of author for correspondence: Department of Genetics, University of Glasgow, Glasgow G12 8QQ, UK. 5 8 1994 © The Company of Biologists Limited 1994 ion transport V-ATPase cyclic AMP forskolin bafilomycin amiloride ouabain bumetanide...