...H. Moser; N. Mair; F. Fresser ABSTRACT In the stretch receptor neurones of the crayfish Astacus astacus , the intracellular pH (pHi), the intracellular Na + concentration ([Na + ] i ) and the membrane potential ( E m ) were measured simultaneously using ion-selective and conventional...

... CaCl 2 ; 10 Hepes, adjusted to pH7.4 with NaOH. In NH 4 + -NAS, NH 4 + was substituted for 20mmol l − 1 Na + . Experiments and calibration of ion-selective microelectrodes were performed at 16°C. Astacus astacus L., bought from a hatchery (Keller, Augsburg, Germany), were dissected...

... limitations of the NH 4 ISM. * To whom correspondence and offprint requests should be sent. 28 01 1991 © 1991 by Company of Biologists 1991 ammonium-selective microelectrodes ammonium intracellular pH regulation stretch receptor crayfish Astacus astacus One...

... mmol I −1 ). The experiments were carried out on the slowly adapting stretch-receptor neurone of the crayfish Astacus astacus L. The stretch receptor with its muscle was isolated as described previously ( Brown et al . 1978 ). The preparation was pinned on the bottom of a flow-through chamber...