...John M. Anderson; Steven C. Hand ABSTRACT Four lines of Drosophila melanogaster were created that expressed transgenes encoding selected late embryogenesis abundant (LEA) proteins originally identified in embryos of the anhydrobiote Artemia franciscana . The overall aim was to extend our...

...Allison M. King; Jantina Toxopeus; Thomas H. MacRae Females of the crustacean Artemia franciscana produce either motile nauplii or gastrula stage embryos enclosed in a shell impermeable to nonvolatile compounds and known as cysts. The encysted embryos enter diapause, a state of greatly reduced...

... in animal mitochondrial gene expression. We have used embryos of the brine shrimp Artemia franciscana to test hypotheses regarding message stability and polyadenylation under conditions simulating anoxia-induced quiescence. In response to anoxia, these embryos undergo a profound and acute metabolic...

...Ana Martinez-Lamparero; Marie-Carmen Casero; Javier Ortiz-Caro; Leandro Sastre SUMMARY Artemia franciscana embryos can suspend their development and metabolism at the gastrula stage to enter a state of cryptobiosis, forming cysts. Embryonic development and metabolism can be resumed under favorable...

...Brian D. Eads; Steven C. Hand SUMMARY In response to anoxia, embryos of the brine shrimp Artemia franciscana are able coordinately to downregulate metabolism to levels low enough to permit survival for several years at room temperature. In addition to dramatic decreases in free ATP levels and heat...

...J. I. Spicer; M. M. El-Gamal ABSTRACT The ability to regulate O 2 uptake during exposure to acutely declining O 2 tensions developed early (stage 6) in the brine shrimp Artemia franciscana and co-occurred with the appearance of a functional heart and gills. Culture under chronic hypoxia resulted...

... shrimp ( Artemia franciscana ) embryos, oxygen deprivation induces a reversible quiescent state that can be tolerated for several years with substantial survivorship. A global arrest of cytoplasmic translation accompanies the transition into anoxia, and rates of protein synthesis in mitochondria from...

...A. H. Warner; S. A. Jackson; J. S. Clegg ABSTRACT Hydrated encysted embryos of the brine shrimp Artemia franciscana have the ability to withstand years in anaerobic sea water using metabolic strategies that enable them to inactivate all cell metabolic activities and then to resume development when...

...James S. Clegg ABSTRACT Encysted gastrula embryos of the crustacean Artemia franciscana have acquired an array of adaptations that enable them to survive a wide variety of environmental extremes.The present paper shows that at least 60 % survive 4 years of continuous anoxia at physiological...

...Thomas J. Anchordoguy; Steven C. Hand ABSTRACT Encysted gastrulae of Artemia franciscana are known to enter a reversible state of quiescence promoted by anoxia, during which the half-life of cytochrome oxidase is prolonged up to 77-fold. This observation suggests that proteolytic pathways within...

... d’Artemia salina à l’anoxie . C.R. hebd. Séanc. Acad. Sci. Paris D 264 , 998 – 1000 . Hand , S. C. ( 1990 ). Heat dissipation during long-term anoxia in Artemia franciscana embryos: identification and fate of metabolic fuels . J. comp. Physiol . B 160 , 357 – 363 . 10.1007...

... cellular RNAs (e.g. rRNA, tRNA, mRNA) are present in the phenol-extracted preparation. Fig. 1. (A) Levels of total extracted RNA in aerobic, anoxic and aerobic acidotic Artemia franciscana embryos. The separate experiments for anaerobic dormancy and aerobic acidosis each included a set of aerobic...