Lung mechanics in juvenile and adult Chelonoidis carbonarius (Spix, 1824)

Testudines possess a rigid shell that influences the mechanics of the respiratory system. We studied respiratory mechanics in the terrestrial red-footed tortoise Chelonoidis carbonarius (Cryptodira), comparing juvenile individuals with a less ossified and more flexible carapace to adults with a well-ossified rigid shell. Combined with these ontogenetic differences, we analyzed respiratory system mechanics with animals in a supine and a prone position, as well as in the isolated lungs, to evaluate the impact of the viscera onto breathing mechanics. To do so, we used established protocols to measure pulmonary volumes (i.e., resting, V_Lr; and maximum, V_Lm), static (C_stat) and dynamic (C_dyn) compliances, and the work of breathing (W). We observed that isolated lungs displayed increased V_Lr, V_Lm, C_stat,C_dyn and decreased W. Additionally, pulmonary volumes, compliances, and W were affected by evaluated positions, such as a smaller V_Lr in a supine position. C_dyn and W showed a volume dependency while frequency had less influence on these variables. At similar levels of ventilation, juveniles showed a lower W than adults when standardized by body mass, but similar W when standardized by V_Lr. Clear ontogenetic changes could be observed in breathing mechanics between juvenile and adult C. carbonarius. While these differences might largely be explained by variations in shell ossifications, other explanations such as differences in visceral proportions or developmental degree of the post-pulmonary septum should also be taken into account.