The glycolytic oscillator, mainly studied in yeast, is described with respect to its overall dynamic and biochemical properties and the kinetics of its master enzyme phosphofructokinase. Biochemical and kinetic analyses are complemented by analysis of mathematical models. In addition to the discussion of structure and function of the glycolytic oscillator under homogeneous spatial conditions, recent theoretical and biochemical experiments demonstrating spatial pattern formation are also discussed, followed by a presentation of physiological viewpoints.

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