A concentrative uptake mechanism for L-glutamate with the following characteristics has been identified in the abdominal nerve cord: 1. The uptake can be divided into Na+-sensitive and Na-plus-insensitive components. 2. The Na-plus-sensitive component showed the typical saturation kinetics of a carrier mediate process. It had a V of 15.9 x 10(6) times 10(6) muM/mg wet weight/min and a Km of 0-33 mm. Its magnitude was proportional to the first power of the Na-plus concentration of the medium. The uptake was specific for L-dicarboxylic amino acids and was sensitive to the presence of metabolic inhibitors. 3. The Na-plus-insensitive component was linearly related to the glutamate concentration of the medium. An isosmotic saline is described for use with the isolated intact abdominal nerve cord of P. americana.
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JOURNAL ARTICLE| 01 February 1975
The uptake of L-glutamate by the central nervous system of the cockroach, Periplaneta americana
Online Issn: 1477-9145
Print Issn: 0022-0949
J Exp Biol (1975) 62 (1): 55–67.
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PD Evans; The uptake of L-glutamate by the central nervous system of the cockroach, Periplaneta americana. J Exp Biol 1 February 1975; 62 (1): 55–67. doi: https://doi.org/10.1242/jeb.62.1.55
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