Altered food landscapes contribute importantly to wildlife disease dynamics and may play a critical role in host heterogeneity in disease outcomes through changes in host diet composition. We explored the effects of dietary macronutrient composition on disease pathology and feeding behavior of canaries (Serinus canaria domestica) infected with Mycoplasma gallisepticum (MG). In the first experiment, we provided canaries with isocaloric diets composed of identical ingredients that varied in macronutrient content (high protein or high lipid) then MG- or sham-inoculated birds. In the second experiment, we offered both diets to canaries before and after MG or sham inoculation. In experiment one, high-protein diet birds consumed more food than high-lipid diet birds and experienced a more pronounced decrease in food intake after infection. High-protein diet birds were more tolerant to MG infection, exhibiting reduced pathology when compared with high-lipid diet birds, despite the two treatments having similar levels of MG-specific antibodies and MG loads. When birds had access to both diets, they consumed more of the high-protein diet and experienced pathology for less time than lipid- or protein-restricted birds. These results highlight that macronutrient makeup of the diet can shape vertebrate host tolerance and pathology, which has direct implications for host–pathogen transmission dynamics.

Diet can be important to a host's susceptibility to infection, severity of pathology ability to efficiently clear an infection (Hall et al., 2009; Unckless et al., 2015; Napier et al., 2019; Gomes et al., 2023). The effect of food availability and composition on disease dynamics within and across species has become increasingly critical to understand as more anthropogenic food resources are introduced to natural and altered environments (Becker et al., 2018). Changes in land use and availability of resources can result in emerging disease hotspots, primarily by altering species diversity and increasing interactions within and among species (Plowright et al., 2011;,Hosseini et al., 2017; Becker et al., 2018). However, aside from agriculturally important livestock, few ecologically based studies have focused on how shifts in diet composition influence individual disease outcomes, despite the important role individual-level disease outcomes play in epidemic dynamics (Hite and Cressler, 2018).

While nutritional resources are critical to host immune function, those resources are also accessible to pathogens for growth and replication (Grieger and Kluger, 1978; Cornet et al., 2014; Cressler et al., 2014). Hosts can restrict pathogen access to nutrients (i.e. starve the pathogen) by undergoing self-induced anorexia (Adamo, 2006). However, hosts also require nutritional resources for mounting a robust immune response; thus, a reduction in nutritional intake may have immunological or energetic costs for the host that affect disease outcomes. Researchers have proposed that relationships between food resources and infection outcomes vary across systems and contexts (Hall et al., 2009; Cressler et al., 2014; Hite and Cressler, 2018; Pfenning-Butterworth et al., 2023). In some situations, host resources are diverted and used for pathogen growth (pathogen priority hypothesis), making anorexia advantageous, and in other situations resources are used directly by the host for immune responses (immune priority hypothesis), making anorexia costly (Cressler et al., 2014). In vertebrates, abundance of nutritional resources tends to favor hosts (Cressler et al., 2014), presumably because resources for parasites or pathogens are abundant in vertebrate hosts, such that additional resources have little direct benefit to the parasites and pathogens (but see Cornet et al., 2014). Instead, additional resources simply improve host immune responses to diminish parasite and pathogen load. However, robust immune responses can also come at a cost to the host by increasing inflammation and tissue damage (Graham et al., 2005; Sorci and Faivre, 2009; Long and Graham, 2011). For example, one study determined that canaries infected with Plasmodium relictum with supplemental food had greater pathology than non-supplemented canaries, whereas parasite growth was greatest in non-supplemented canaries (Cornet et al., 2014). These studies underscore the complexity of relationships that exist between resource availability and host and pathogen outcomes.

Abundance of resources is certainly important to host–pathogen interactions, but so is the nutritional makeup of those resources. The nutritional composition of the diet contributes to how effectively immune processes eliminate pathogens (Cunningham-Rundles et al., 2005; Amar et al., 2007). Manipulative studies in an ecological context show the importance of dietary macronutrients on immune processes to ultimately affect individual disease outcomes (Klasing, 2007; Cotter et al., 2011; Povey et al., 2009, 2013). In one study, caterpillars experimentally infected with a baculovirus, Spodoptera exempta, preferred high-protein diets when given the option. The increase in consumption of high-protein diets led to higher rates of survival from infection than the caterpillars given a high-carbohydrate diet (Povey et al., 2013). Another study in poultry demonstrated how variation in dietary lipids affected immune responses by modifying leukocyte production (Friedman and Sklan, 1995) and lipid composition can have direct effects on inflammatory responses (Klasing, 1998). However, high-lipid diets can increase mortality rates in invertebrates during some infections (Adamo et al., 2008). Variation in immune responses based on macronutrient composition in the diet is likely a result of a host requiring different nutrition to produce robust immune responses for clearing specific pathogens (Hite and Cressler, 2018). These studies demonstrate that outcomes of host–pathogen interactions depend on the macronutrient breakdown of food resources, presenting the opportunity for macronutrient-specific changes in host feeding during infection and disease outcomes as seen in some invertebrate systems (Povey et al., 2013; Sieksmeyer et al., 2022). There is some evidence that vertebrates, which will selectively eat plants with antiparasitic compounds during parasitic infection (Huffman and Seifu, 1989; Hutchings et al., 2003), may also shift macronutrient intake during infection, as noted by reduced protein intake in zebra finches after injection with a non-pathogenic antigen (Love et al., 2024). This finding raises the possibility that vertebrate hosts could selectively consume macronutrients that improve host immune function (the immunity priority hypothesis; Cressler et al., 2014), while still reducing important macronutrients for pathogen growth (pathogen priority hypothesis; Cressler et al., 2014).

The goal of this study was to explore the effects of macronutrients on disease outcomes, e.g. disease severity and recovery time after exposure to a bacterial pathogen in a vertebrate host–pathogen system. Similar research has been done using a non-pathogenic novel antigen (Love et al., 2024); however, a true pathogenic infection could lead to different results because the immunological pathways used to clear the antigen by the host can be different (Koch et al., 2018; Macpherson et al., 2001). Often, studies exploring immune and diet interactions change food abundance or food types (Tschirren et al., 2007; Hall et al., 2009; Cornet et al., 2014; Nwaogu et al., 2020), making it difficult to pinpoint the effects of macronutrients on disease outcomes. This study sought to improve our understanding of the role of macronutrients in shaping vertebrate disease pathology during infection and why some animals exhibit selective or reduced feeding during illness. We conducted two experiments aimed at answering different but complementary questions: (1) how will macronutrient-specific diets affect disease severity and immune response in a vertebrate host–pathogen system and (2) will dietary preference shift during an infection?

To test our hypotheses, we used a common avian host–pathogen system, domestic canaries infected with the bacterial pathogen Mycoplasma gallisepticum (MG). MG can infect several songbird species and tends to cause severe conjunctival swelling and lethargy in several species belonging to the Family Fringilidae, including canaries and house finches (Hawley et al., 2011; Dhondt et al., 2008; Love et al., 2021). Although MG is most well studied in wild house finches, domestic canaries are proving to be a useful lab model species because they thrive in lab conditions and experience similar pathology and pathogen load when infected with MG to house finches (Hawley et al., 2011; Love et al., 2021; Sauer et al., 2024). Earlier studies in our lab indicated that zebra finches reduce protein consumption but maintain lipid consumption during a non-pathogenic immune challenge (Love et al., 2024), suggesting that lipids may benefit host immune responses. Hosts may reduce protein consumption because protein is more likely to contain iron than other macronutrients and iron is essential for some pathogens (Kluger and Rothenburg, 1979; Cassat and Skaar, 2013). Thus, reducing protein consumption during an immune threat could limit pathogen growth. Based on these data and the immunity and pathogen priority hypotheses, we predicted in the first experiment, that birds fed a high-lipid diet would clear MG faster and have lower pathogen load than birds fed a high-protein diet. Because of these benefits to the host, we predicted that birds in the second experiment would prefer a high-lipid diet during MG infection.

Bird housing

Canaries were housed in an ABSL-1 biosafety room on a 14 h light:10 h dark cycle throughout both experiments. Birds were housed in wire cages (∼61×41×41 cm) that were divided into two units with each unit containing one bird to allow assessment of individual food consumption. Each housing space contained two plastic perches, a water dish and a food dish in the first experiment. The housing space contained an additional food dish for the second experiment (diet preference) and the water dish was placed completely within the cage. To prevent contamination of control birds by MG-infected birds, which is primarily transmitted through direct contact and fomites in finches (Dhondt et al., 2007), a plastic partition divided the room, with controls held on one side of the partition and MG-infected birds kept on the other. All procedures were approved by the University of Arkansas Institute of Animal Care and Use Committee.

Experimental design and time line

Experiment 1

Individually housed female canaries, Serinus canaria domestica (Linnaeus 1758) (N=37), were provided with either a high-lipid (n=22) or high-protein (n=20) diet 17 days prior to inoculation to acclimate treatment groups to the diets. Following acclimation to the diets, birds were inoculated with either Frey's media or MG, resulting in four treatments: lipid MG (n=13), lipid control (n=9), protein MG (n=10), protein control (n=10). Two birds in the lipid MG treatment and one in the protein MG treatment died during the experiment, so they are not represented at all time points in the dataset. Only females were used in this experiment because they were being used in a subsequent study exploring disease-mediated parental effects on egg attributes. We weighed birds, measured fat scores, assessed eye inflammation and measured various immune endpoints (e.g. white blood cell count, pathogen load, MG-specific antibody levels) throughout infection. Eyes were scored for inflammation and swabbed to measure pathogen load prior to infection and every other day post-inoculation until 35 days post infection. Body mass and fat scores were collected prior to infection and at days 7, 14, 21 and 35 post-infection. We collected blood samples from birds immediately prior to infection and at days 7, 14 and 21 post-infection. The blood samples were used to assess hematocrit and white blood cell differential counts, and to quantify MG antibody concentrations in birds.

Experiment 2

Canaries (N=25) were housed individually and provided with both a high-lipid and a high-protein diet daily throughout the experiment. Birds were acclimated to the diet for 17 days, then inoculated with either Frey's media (n=6 females, n=6 males) or MG (n=6 females, n=7 males). A mix of males and females was used in this experiment because we did not have enough females to keep this consistent with the design of experiment 1. Four MG-infected birds died during the first week after MG inoculation, three males and one female. We recorded body mass, fat stores, conjunctiva inflammation and other immune endpoints (e.g. white blood cell count, pathogen load, MG-specific antibody levels) throughout infection. Prior to and every 2–3 days after infection, we scored eye inflammation, then bilaterally swabbed eye conjunctiva to quantify pathogen load. Body mass and fat scores were collected prior to infection and 7, 14, 21 and 35 days post-infection. We collected blood samples prior to infection and at days 7, 14 and 21 post-infection. As in experiment 1, blood samples were used to assess hematocrit, determine the relative abundance of white blood cells and quantify concentrations of MG-specific antibodies.

Diet composition and monitoring of feeding

The diets we chose fall within the range of naturally occurring and anthropogenic foods available to birds, which can vary widely in macronutrient composition (lipid:protein ratios can vary from 7.5:1 to 1:64: Newton, 1967; Bellrose and Holm, 1994; Coogan et al., 2018). For instance, common seed mixes provided in bird feeders have lipid:protein ratios ranging from 4.4:1 to 1:3.5. Birds can also seasonally shift lipid or protein intake according to resource availability, life history stage or through selective feeding (Newton, 1967; Barlein, 1990;,Bellrose and Holm, 1994; Coogan et al., 2018). In the first experiment, birds were fed daily a 24 g isocaloric food bar that was either lipid rich (4:1 lipid to protein ratio) or protein rich (1:4 lipid to protein ratio). The two diets contained varying proportions of egg whites, egg yolks, hulled millet and cod liver oil, and were congealed together with agar. In the second experiment, birds received a bar of each diet placed in separate food dishes. The dishes were on either side of the cage front, and we randomized which side received which diet for each rack of cages at the beginning of the experiment. Diet placement did not change during the experiment. Diets were weighed and replaced daily shortly after lights came on. For both experiments, three food bars for both diets were placed in the bird room and weighed daily to account for desiccation, and this mass was then averaged and subtracted from the amount of diet consumed by each bird.

Inoculations and monitoring of disease severity

After acclimation to diets, birds were inoculated with MG or a control solution. We inoculated MG-treated birds in both experiments bilaterally with MG inoculum (VA1994; E. Tulman, University of Connecticut) in their palpebral conjunctiva with 25 μl containing 5×107 CCU ml−1 of MG inoculum diluted 16.9% in Frey's media. Birds assigned to the control treatments in both experiments were inoculated with 25 μl of Frey's media. Throughout the duration of infection, inflammation of the conjunctiva, a measure of disease severity, was scored on a scale of 0 to 3 (Hawley et al., 2011); higher scores represent a greater degree of disease pathology. Both eyes of each bird were given a score and these were summed to determine a total eye score value (Hawley et al., 2011). Birds were marked as recovered on the day eye score dropped to zero and remained zero for the rest of the experiment. Disease severity was also monitored by recording changes in body mass and fat scores. Fat scores were measured on a scale of 0 to 3 and categorized by how much visible adipose tissue was present in the interclavicular fossa of the birds. A low fat score value indicates only a small trace or lack of visible fat tissue and higher values indicate more fat tissue present.

Antibody assays

After blood samples were collected, they were microcentrifuged at 3500 rpm, and the plasma was removed and stored in a −20°C freezer for future analysis of MG antibody concentrations. Serum antibodies were quantified using the IDEXX MG antibody enzyme-linked immunosorbent assay test kit (IDEXX, cat no. 99-06729). A blocking step was added to the original assay kit's protocol, with the addition of 300 μl of 1% bovine serum albumin (Pierce 10× BSA; Thermo Fisher Scientific) in phosphate-buffered saline to room temperature plates before they were incubated. All plates were washed 3 times with phosphate-buffered saline containing 0.05% Tween 20 using an ELx50 plate washer (BioTek). Serum samples were diluted 1:50 in sample buffer and were then plated to be run in duplicate. The intensity of light absorbed by the serum samples was measured at 630 nm using a spectrophotometer and an ELISA value was then calculated.

Pathogen load

We determined pathogen load using quantitative PCR following the procedure outlined by Grodio et al. (2008) that targets the mgc2 gene of MG. Sterile cotton swabs were dipped in tryptose phosphate broth and used to swab conjunctiva in both eyes of each bird for 5 s each. The tips of the swabs were then cut off and placed in 300 μl of the tryptose phosphate broth and frozen in a −20°C freezer. Qiagen DNeasy 96 Blood and Tissue kits (Qiagen, Valencia, CA, USA) were used to extract genomic DNA, and primers and probe that target mgc2 were used to assess pathogen load. The total liquid volume of 15 μl included 7.5 μl of Primetime Master Mix (Bio-Rad Laboratories, Hercules, CA, USA), 3.525 μl DNase-free water, 3 μl of DNA sample, 0.375 μl of forward and reverse primers and 0.225 μl of 10 μmol l−1 MG probe. A BioRad CFX-96 machine was used for cycling at 95°C for 3 min, followed by 40 cycles of 95°C for 3 s, and then 60°C for 3 s. The ramp rate of the machine was set to 0.5 s−1. The mgc2 expression values were a summed total of both conjunctiva of each individual bird and sample day. Final concentrations were calculated by multiplying 3 μl, the amount of DNA sample used, by 66.666, to be comparable to the 200 μl that was produced from the elution step. Determined values for mgc2 were then log transformed to reduce the large outputs that were generated.

Statistical analyses

Prior to all statistical analyses, data were checked for normality and homoscedasticity. All statistics were conducted with R version 4.3.2 in R Studio (http://www.rstudio.com/). To test for the effects of diet, MG exposure, time and their interactions in the first experiment and sex, MG exposure, time and their interactions in the second experiment on food intake (g), body mass (g), fat score and hematocrit (%), we conducted linear or normally distributed generalized linear mixed-effects models followed by ANOVA (lme4 and car packages). To test for effects of the same predictors on relative white blood cell abundance and heterophil:lymphocyte ratio, we conducted separate generalized linear mixed-effects models with varying distributions followed by ANOVA (see https://github.com/erinsauer/Perrine-et-al-MG-diet for details). To test for the effect of diet, time and their interaction in the first experiment and sex, time and their interaction in the second experiment on total eye score in MG-exposed birds, we conducted a negative binomial distributed generalized additive mixed model that included a smoothing spline to model the non-linear effects over time (mgcv package). We further explored the effects of diet from the first experiment on pathology recovery time (days) by conducting a Cox proportional hazard model (survival package). To test for the effects of diet, time and their interaction in the first experiment and sex, time and their interaction in the second experiment on log10-transformed pathogen load and MG-specific antibody level (optical density), we conduced normally distributed generalized linear mixed-effects models followed by ANOVA. All mixed-effects models included a random intercept for bird identity. In both experiments, we analyzed food consumption two ways, by day and by week, which yielded similar results. We chose to use weekly feeding patterns because they were easier to compare visually and capture feeding behavior at distinct phases of infection: pre-infection, peak infection, early recovery and late recovery. See Figs S1 and S3 for daily feeding patterns. All linear and generalized linear mixed models were assessed for normality, homoscedasticity and temporal autocorrelation in the residuals using the DHARMa package while generalized additive mixed models were assessed using the gam.check function in the mgcv package. All stats output are reported in tables in GitHub (https://github.com/erinsauer/Perrine-et-al-MG-diet).

Experiment 1

Generally, birds fed the high-protein diet consumed more food each week than those on the high-lipid diet. In the week immediately following inoculation, food consumption decreased sharply for MG-inoculated high-protein birds and moderately for the MG-inoculated high-lipid diet birds relative to controls (infection×diet×week: χ2=5.10, d.f.=1, P=0.02; diet×week: χ2=5.97, d.f.=1, P=0.01; diet: βprotein=7.31±9.98, χ2=3.30, d.f.=1, P=0.07; week: β=0.74±1.10, χ2=2.80, d.f.=1, P=0.09; Fig. 1A). There were no other significant main or interactive effects on food consumed (P>0.1).

Fig. 1.

Experiment 1: disease pathology and feeding behavior of female canaries (Serinus canaria domestica) fed a high-protein or high-lipid isocaloric diet and later inoculated with Mycoplasma gallisepticum or control solution. Birds received either high-protein (1:4 lipid:protein) or high-lipid (4:1 lipid:protein) diet bars and were inoculated with Mycoplasma gallisepticum (MG) or Frey's media (control) 17 days later. The day of inoculation was termed day 0 and endpoints were monitored for 4 weeks after MG inoculation. Birds remained on the diet for the duration of the experiment. (A) Weekly food intake, (B) furcular fat scores, (C) percentage hematocrit, (D) Mycoplasma gallisepticum (MG)-specific antibody production (optical density, OD), (E) conjunctival swelling, represented by a score of swelling severity, and (F) log10-transformed MG pathogen load. Only MG-infected birds are depicted in panels related to disease outcome (antibody production, eye swelling and pathogen load; D–F). Lipid control, n=13; lipid MG, n=9; protein control, n=10; protein MG, n=10. Points represent raw data with smoothed (A,B,D–F) or linear (C) average trend lines of the raw data surrounded by 95% confidence interval bands in gray.

Fig. 1.

Experiment 1: disease pathology and feeding behavior of female canaries (Serinus canaria domestica) fed a high-protein or high-lipid isocaloric diet and later inoculated with Mycoplasma gallisepticum or control solution. Birds received either high-protein (1:4 lipid:protein) or high-lipid (4:1 lipid:protein) diet bars and were inoculated with Mycoplasma gallisepticum (MG) or Frey's media (control) 17 days later. The day of inoculation was termed day 0 and endpoints were monitored for 4 weeks after MG inoculation. Birds remained on the diet for the duration of the experiment. (A) Weekly food intake, (B) furcular fat scores, (C) percentage hematocrit, (D) Mycoplasma gallisepticum (MG)-specific antibody production (optical density, OD), (E) conjunctival swelling, represented by a score of swelling severity, and (F) log10-transformed MG pathogen load. Only MG-infected birds are depicted in panels related to disease outcome (antibody production, eye swelling and pathogen load; D–F). Lipid control, n=13; lipid MG, n=9; protein control, n=10; protein MG, n=10. Points represent raw data with smoothed (A,B,D–F) or linear (C) average trend lines of the raw data surrounded by 95% confidence interval bands in gray.

Regardless of treatment, all birds were similar in mass (Fig. S1) and had similar furcular fat scores (Fig. 1B) prior to MG infection. Body mass decreased during the middle of the experiment, then rebounded, but was not affected by infection or diet (day: β=−0.03±0.02, χ2=3.13, d.f.=1, P=0.08). Hematocrit differed over time by diet, with higher hematocrit in birds fed the high-protein diet at the start of the experiment, but high-lipid diet birds caught up by the end of the experiment (day×diet: χ2=5.31, d.f.=1, P=0.02) (Fig. 1C). There were no other significant main or interactive effects on body mass, fat score or hematocrit (P>0.1).

Macronutrient composition of the diet also significantly affected eye score, with more severe swelling in the birds fed the high-lipid diet as compared with birds fed the high-protein diet (Diet: t=−2.03, P=0.04; Fig. 1E). The median time to recovery of conjunctiva swelling of birds was 21 days in MG-infected birds fed the high-protein diet and 30 days for MG-infected birds fed the high-lipid diet [exp(coef)protein=3.69±0.58, z=2.24, d.f.=1, P=0.03]. There was a significant effect of time on eye swelling (day: F=30.2, edf=4.87, P<0.0001; Fig. 1E), in which MG-infected birds from both diets exhibited more severe swelling during the first 10 days of infection and swelling diminished in the days after peak infection.

Infected birds in both diet treatments exhibited high pathogen loads in the first week of infection, which decreased by 14 days post infection (β=−0.41±0.04, χ2=171.05, d.f.=1, P<0.001; Fig. 1F) and this did not differ with diet treatment (P>0.4). Similarly, infected birds in both diet treatments experienced a significant increase in MG-specific antibodies after infection (β=0.01±0.001, χ2=25.93, d.f.=1, P<0.001; Fig. 1D) and this did not differ among diets (P>0.3).

We found significant effects of infection and diet on the production of different white blood cell types. In general, basophils remained low throughout the entire experiment, with individuals exhibiting 0–1 basophils at most time points. However, because one individual in the MG protein treatment had five basophils on day 14 post-inoculation (all other birds in this treatment on day 14 had 0–2 basophils), this generated a significant infection×diet×day interaction on basophil production (χ2=4.65, d.f.=1, P=0.03; Fig. S2). In all groups, eosinophils increased from the day of inoculation to day 7 post-inoculation and remained elevated to day 21 post-inoculation (β=0.04±0.02, χ2=24.64, d.f.=1, P<0.001; Fig. S2) and high-lipid diet birds had more eosinophils than high-protein diet birds (βprotein=−0.51±0.42, χ2=4.04, d.f.=1, P<0.05; Fig. S2). Monocytes trended towards increasing throughout the course of the experiment in all birds (β=0.04±0.03, χ2=3.14, d.f.=1, P=0.08; Fig. S2). On average, birds fed the high-protein diet experienced significantly increased numbers of lymphocytes (βprotein=4.07±3.19, χ2=9.80, d.f.=1, P=0.002; Fig. S2) compared with birds fed the high-lipid diet. Lymphocytes also generally decreased in abundance over time (β=−0.59±0.18, χ2=24.80, d.f.=1, P<0.001; Fig. S2). Heterophil production increased slightly with time (β=0.01±0.01, χ2=8.61, d.f.=1, P=0.003; Fig. S2) and high-protein diet birds trended towards lower abundance of heterophils than high-lipid diet birds (βprotein=−0.51±0.26, χ2=3.55, d.f.=1, P=0.06; Fig. S2). The heterophil:lymphocyte ratio was not affected by the infection status of birds but did increase with time across all treatments (β=0.02±0.01, χ2=13.49, d.f.=1, P<0.001; Fig. S2) and tended to be lower in high-protein diet birds than in high-lipid diet birds (βprotein=−0.67±0.31, χ2=5.07, d.f.=1, P=0.02; Fig. S2). There were no other significant main or interactive effects on white blood cell relative abundance or heterophil:lymphocyte ratio (P>0.1).

Experiment 2

Infected birds in experiment 2 ate less food than control birds and all birds increased food intake over time (week: β=10.17±1.96, χ2=77.37, d.f.=1, P<0.001; infection: βMG=−17.23±9.01, χ2=7.53, d.f.=1, P=0.006; Fig. 2A; Fig. S2). We found the same pattern in feeding when we compared intake of the different diets; infected birds ate less protein diet (βMG=−0.03±0.25, χ2=5.77, d.f.=1, P=0.02; Fig. 2B) and lipid diet during the experiment than control birds (βMG=−3.28±6.15, χ2=3.75, d.f.=1, P=0.05; Fig. 2C), and all birds increased protein and lipid intake over time (protein: β=−0.03±0.01, χ2=26.82, d.f.=1, P<0.001; lipid: β=6.48±1.26, χ2=57.50, d.f.=1, P<0.001). We also found that females tended to consume more food overall and more protein each week than males (total: χ2=2.64, d.f.=1, P=0.10; protein: χ2=5.77, d.f.=1, P=0.02). There were no other main or interactive effects of sex on canary feeding patterns (χ2<2.5, P>0.11).

Fig. 2.

Experiment 2: feeding behavior of male and female canaries provided with both the high-lipid and high-protein diet bars and later inoculated with Mycoplasma gallisepticum or control solution. Birds were provided with two isocaloric diet bars, one that was high in protein (1:4 lipid:protein) and one that was high in lipid (4:1 lipid:protein), and were inoculated with Mycoplasma gallisepticum (MG) or Frey's media (control) 17 days later. The day of inoculation was termed day 0 and endpoints were monitored for 4 weeks after inoculation. (A) Weekly total food intake, (B) consumption of the high-protein bar and (C) consumption of the high-lipid diet bar. Controls: n=6 females, n=6 males; MG infected: n=6 females, n=7 males. Points represent raw data with smoothed average trend lines of the raw data surrounded by 95% confidence interval bands in gray.

Fig. 2.

Experiment 2: feeding behavior of male and female canaries provided with both the high-lipid and high-protein diet bars and later inoculated with Mycoplasma gallisepticum or control solution. Birds were provided with two isocaloric diet bars, one that was high in protein (1:4 lipid:protein) and one that was high in lipid (4:1 lipid:protein), and were inoculated with Mycoplasma gallisepticum (MG) or Frey's media (control) 17 days later. The day of inoculation was termed day 0 and endpoints were monitored for 4 weeks after inoculation. (A) Weekly total food intake, (B) consumption of the high-protein bar and (C) consumption of the high-lipid diet bar. Controls: n=6 females, n=6 males; MG infected: n=6 females, n=7 males. Points represent raw data with smoothed average trend lines of the raw data surrounded by 95% confidence interval bands in gray.

Birds infected with MG weighed less than control birds (βMG=−4.57±2.35, χ2=4.04, d.f.=1, P=0.04; Fig. 3B) and had lower hematocrit (βMG=−0.85±3.08, χ2=4.25, d.f.=1, P=0.04; Fig. 3C). Although females were heavier than males (βmale=−5.09±2.34, χ2=3.81, d.f.=1, P=0.05), males had higher hematocrit than females (βmale=8.78±3.05, χ2=10.26, d.f.=1, P=0.001). Infected birds lost fat stores later in infection, whereas control birds tended to gain fat stores (infection×day: χ2=3.81, d.f.=1, P=0.05; Fig. 3A). Females also had more fat stores than males at the beginning of the experiment, but males gained fat later in the experiment (sex: βmale=−1.10±0.40, χ2=6.43, d.f.=1, P=0.01; sex×day: χ2=7.48, d.f.=1, P=0.006). There were no other significant main or interactive effects on body mass, fat stores or hematocrit of canaries (P>0.10).

Fig. 3.

Experiment 2: disease pathology of male and female canaries provided with both the high-lipid and high-protein diet bars and later inoculated with Mycoplasma gallisepticum or control solution. Birds were treated as in Fig. 2. (A) Furcular fat stores, (B) body mass, (C) percentage hematocrit and (D) and conjunctival swelling, represented by a score of swelling severity. Only MG-infected birds are depicted in the panel related to disease outcome (eye swelling; D). Controls: n=6 females, n=6 males; MG-infected canaries: n=6 females, n=7 males. Points represent raw data with smoothed (A,B,D) or linear (C) average trend lines of the raw data surrounded by 95% confidence interval bands in gray.

Fig. 3.

Experiment 2: disease pathology of male and female canaries provided with both the high-lipid and high-protein diet bars and later inoculated with Mycoplasma gallisepticum or control solution. Birds were treated as in Fig. 2. (A) Furcular fat stores, (B) body mass, (C) percentage hematocrit and (D) and conjunctival swelling, represented by a score of swelling severity. Only MG-infected birds are depicted in the panel related to disease outcome (eye swelling; D). Controls: n=6 females, n=6 males; MG-infected canaries: n=6 females, n=7 males. Points represent raw data with smoothed (A,B,D) or linear (C) average trend lines of the raw data surrounded by 95% confidence interval bands in gray.

Eye swelling, pathogen load and MG antibodies increased in infected birds after inoculation with MG. Eye swelling and pathogen load were greatest 5–10 days after infection, then subsided (eyescore: F=20.84, edf=4.80, P<0.001; pathogen load: χ2=44.86, d.f.=1, P<0.001; Figs 3D, 4B), whereas MG antibodies steadily increased after inoculation (β=0.001±0.001, χ2=18.02, d.f.=1, P<0.001; Fig. 4B). Infected males exhibited greater eye swelling (t=4.04, P<0.001; Fig. 3D) and experienced greater pathogen load than females (βmale=1.35±1.89, χ2=4.59, d.f.=1, P=0.03; Fig. 4B) during infection. Infected males also produced more MG-specific antibodies over time than females (sex×day: χ2=3.56, d.f.=1, P=0.06; Fig. 4A). Aside from one bird that exhibited swollen conjunctiva past the end of the second experiment, infected birds recovered by day 16 post-infection and average recovery time was 11.2±3.0 days.

Fig. 4.

Experiment 2: antibody levels and pathogen load of male and female canaries provided with both the high-lipid and high-protein diet bars and later inoculated with Mycoplasma gallisepticum or control solution. Birds were treated as in Fig. 2. There was a differential effect of sex on (A) MG-specific antibodies and (B) log10-transformed MG load over time in only the Mycoplasma gallisepticum (MG)-exposed canaries. Points represent raw data with average trend lines surrounded by 95% confidence interval bands in gray.

Fig. 4.

Experiment 2: antibody levels and pathogen load of male and female canaries provided with both the high-lipid and high-protein diet bars and later inoculated with Mycoplasma gallisepticum or control solution. Birds were treated as in Fig. 2. There was a differential effect of sex on (A) MG-specific antibodies and (B) log10-transformed MG load over time in only the Mycoplasma gallisepticum (MG)-exposed canaries. Points represent raw data with average trend lines surrounded by 95% confidence interval bands in gray.

The only effect of MG infection on the relative abundance of white blood cells in birds that had both diets available was dependent on bird sex. Monocytes decreased in male birds over time with a peak occurring at day 7 post-infection only in MG-infected males, which aligns with peak pathology, while monocytes in female birds stayed relatively consistent over time, with MG-infected female birds having lower levels than control females (sex×day: χ2=4.52, d.f.=1, P=0.03; sex×infection: χ2=4.37, d.f.=1, P=0.04; Fig. S4). Heterophils increased slightly for female birds over time, regardless of MG exposure, but stayed consistent over time in male birds (sex×day: χ2=4.41, d.f.=1, P=0.04; Fig. S4). Heterophil:lymphocyte ratios varied as a function of sex and time, slightly increasing for females over time but slightly decreasing for males (sex×day: χ2=13.61, d.f.=1, P<0.001; Fig. S4). Similar to experiment 1, the presence of basophils was rare and only one was detected throughout the course of this experiment. There was no significant difference detected in relative abundance of any other white blood cells between control and MG-infected birds or between sexes (P>0.09).

In this study, we used a vertebrate host–pathogen system to shed light on how individual macronutrients affect host disease outcomes and whether these effects are reflected in dietary preference during an infection. Our results indicate that a diet rich in lipids but poor in proteins results in greater disease pathology and reduced recovery time despite similar infection intensity and antibody production (i.e. reduced tolerance) to birds receiving a diet rich in protein but poor in lipids. However, when given the choice, infected birds did not selectively reduce lipids following infection, although they did consume more protein overall regardless of infection status. Our results support the immune priority hypothesis, because macronutrients shaped host immune responses but not pathogen growth. Our results also demonstrate that diet composition has implications for disease outcomes important for transmission through effects on host responses to infection and should be considered in the context of altered food landscapes, whether human induced (e.g. dumpsters, bird feeders, etc.) or naturally occurring (e.g. drought), and wildlife disease dynamics.

A high-protein diet was important to immune support, noted by the faster attenuation of disease pathology as measured by conjunctival swelling (Hawley et al., 2011) in high-protein diet birds than in high-lipid diet birds, though the two groups exhibited similar peak pathology. Conjunctival swelling is an important indicator of transmission in avian MG systems. In house finches, conjunctival swelling correlates positively with conjunctival pathogen load and the likelihood of transmitting MG (Adelman et al., 2013). However, in our system, pathogen load and MG antibody production did not differ between birds fed the two diets, despite the extended pathology in the high-lipid diet birds. This finding has several important implications. First, high-lipid diet birds are more likely to transmit MG because swelling alone is integral to pathogen transmission, perhaps because they are more likely to wipe infected tissues onto feeders or conspecifics (Adelman et al., 2013). However, high-protein diet birds also may be capable of depositing MG onto feeder surfaces, even though the conjunctiva is not heavily swollen, because of high pathogen load. This could alter typical transmission dynamics in a population because disease cues in conspecifics can result in immune priming and behavioral shifts in individuals (Bouwman and Hawley, 2010; Love et al., 2021, 2023). If diet alters the appearance or severity of an important indicator of illness (e.g. swelling), conspecifics may not detect illness in birds eating a protein-rich diet even though they are capable of transmitting the pathogen. Second, high-protein diet birds appear to tolerate infection better than high-lipid diet birds because they show reduced pathology despite having similar pathogen loads and antibody production to birds fed a high-lipid diet. This indicates that diet plays an important role in whether individuals tolerate or resist an infection and suggests that studies exploring how resource availability shapes host–pathogen evolutionary dynamics are needed.

The differences in tolerance and pathology driven by diet that we detected are also relevant to consider in the context of supplemental feeding of wildlife, as studies have shown that when supplemented foods are not carefully considered they can lead to global scale shifts in pathogen transmission (Murray et al., 2016). Resource-driven shifts in immunity can drastically alter epidemics by increasing the likelihood of producing individuals with superspreading abilities, because resource scarcity is more likely to result in hosts with high pathogen loads or extended infections (Hall, 2019). The longer period of conjunctival swelling in the high-lipid diet birds in our study, which experienced protein scarcity, should mean they are more likely to transmit MG than the high-protein diet birds (Sauer et al., 2024).

Although the mechanism behind the faster recovery in high-protein diet birds is unclear, this result could suggest that a certain threshold of protein intake is required prior to and during infection for optimal recovery. Dietary protein is essential for the regulation and activation of both T and B lymphocytes, both of which are important for recovery from an infection (Peng et al., 2007) and some animals are more likely to survive infection when on a high-protein diet (Jahanian, 2009; Lee et al., 2008). We found that high-protein diet birds had greater circulating lymphocytes than high-lipid diet birds. B lymphocytes produce antibodies (LeBien and Tedder, 2008); however, we did not detect a difference in MG antibody production. It is possible that dietary protein may be affecting other immunological pathways that were not investigated, such as cytokines which are produced by T lymphocytes. For instance, increased levels of interleukin-1 act as pro-inflammatory agents (Dinarello, 1997), while other cytokines, such as interleukin-5 and interleukin-10, play an anti-inflammatory role during inflammatory infections (Pirola and Ferraz, 2017). Our microscopy techniques did not distinguish between types of lymphocytes, so it is possible that elevated T lymphocytes, but not B lymphocytes, drove the diet effect on the relative abundance of circulating lymphocytes and led to the production of anti-inflammatory cytokines. We also found that eosinophils were higher in high-lipid diet birds. Eosinophils move into inflamed areas and contribute to the inflammatory response and tissue damage (Behm and Ovington, 2000) and could be contributing to increased conjunctival swelling in high-lipid diet birds or could just be correlated with other aspects of inflammation. High-protein diet birds also had higher hematocrit at the beginning of the experiment, which can indicate greater oxygen carrying capacity, but it is unclear how this might affect pathology. Although we found differences in the underlying immune cells important in responding to an infection, more work is needed to determine the underlying immune mechanisms stimulated by diet that lead to differences in disease outcomes.

There was some evidence that it was disadvantageous to only have access to a high-protein low-lipid diet. Despite high-protein diet birds clearing conjunctival swelling more quickly than high-lipid diet birds, high-protein diet birds had somewhat lower furcular fat stores at the end of the study (Fig. 1B) as compared with high-lipid diet birds. Scoring fat stores is a coarse measure of available fat to the bird, which may have clouded our ability to detect a significant effect of diet on this endpoint. If high-protein diet birds did suffer greater total body fat loss, this suggests that high-protein diet birds, which consumed fewer grams of lipids than high-lipid diet birds (0.84±0.02 g versus 2.44±0.12 g), relied heavily on fat stores to meet the energy demands of fighting the infection and this may be an important cost for clearing the pathogen. We did detect significant fat loss in infected birds with access to both diets (experiment 2), but these birds quickly recovered fat stores by the end of the experiment. Infected birds with access to both diets also recovered faster from conjunctival swelling (11 days) than both the high-protein (16 days) and high-lipid diet birds (25 days) in experiment 1. These results suggest that having access to both diets allowed infected birds to mitigate the more severe disease outcomes that resulted from consuming the macronutrient-deficient diets and there are optimal thresholds for both macronutrients that must be met to both maintain body condition and tolerate infection.

In both experiments, we found that infected birds had lower food intake as compared with controls, a behavioral pattern referred to as illness-induced anorexia, which is commonly observed across vertebrates in response to pathogenic infection (Murray and Murray, 1979; Adelman and Martin, 2009). When birds had access to only one diet, reduced feeding was greater in high-protein diet birds than in high-lipid diet birds (39.11% versus 17.34%), a result consistent with other research in our lab on zebra finches, which reduced protein consumption following an immune challenge with the bacterial endotoxin lipopolysaccharide (Love et al., 2024). In our study, infected birds may have reduced protein intake during infection because of its importance to the pathogen (Kluger and Rothenburg, 1979; Cassat and Skaar, 2013), particularly when they had ample access to dietary proteins prior to infection (Lin et al., 2020). While we expected macronutrient-selective feeding following infection when birds had access to both diets (experiment 2), this did not occur. This result is counter to findings in zebra finches and caterpillars, which both exhibited macronutrient-specific shifts in protein feeding after an immune challenge (reduced protein intake; Love et al., 2024) or infection (increased protein intake; Povey et al., 2009). What we may have detected in canaries is a threshold requirement for proteins. Generally, birds preferred to eat the high-protein diet, and the high-lipid diet birds in experiment 1 already had lower protein intake (0.61±0.03 g in high-lipid diet birds versus 3.34±0.08 g in high-protein diet birds), and therefore they did not need to or could not reduce protein intake much further. Regardless, these data indicate that birds can discern differences in the macronutrient make-up of diet and feed in ways that maximize immunity, but they may not be able to shift feeding in response to an infection aside from general illness-induced anorexia. Future studies should explore whether birds can exhibit prophylactic shifts in feeding behaviors in response to heightened infection risk (Love et al., 2024).

In the second experiment, we detected several sex effects on the behavioral and immunological endpoints that we measured. Our study was not focused on sex differences in immune responses, and males were included for logistical reasons, but the findings warrant some discussion. Females ate more food and more protein, but not lipids, than males during the experiment. Perhaps, as a result, females weighed more and had more fat stores at the beginning of the experiment than males. Females also experienced lower pathology, pathogen growth and MG antibody production, but males had higher hematocrit. It is interesting that the sex that consumed more protein also had the lowest disease pathology and greatest tolerance, which is consistent with the patterns we detected in the high-protein diet birds in experiment 1. It is still unclear what mechanism may drive this. Differences in circulating levels of eosinophils and lymphocytes did not exist between the sexes as they did between diet treatments, though males did have a larger monocyte response 7 days after infection and monocytes contribute to local and systemic inflammation (Kurihara et al., 1997). Although the sex-driven changes in white blood cells are different from diet-driven changes, in both instances the groups expressing the greatest pathology had greater relative abundance of white blood cells associated with inflammation. Importantly, the sex-specific differences in pathology and tolerance we detected can lead to sex-biased disease dynamics (Sauer et al., 2024).

This study broadens our understanding of the dynamics between diet and a vertebrate host's response to infection with a replicating pathogen. Our findings indicate that consuming higher levels of dietary protein is important for clearing the physical symptoms of an infectious pathogen by increasing host tolerance but not host resistance. These findings are important because they demonstrate that the food landscape can shape individual health outcomes in response to infection, which should have direct consequences for pathogen shedding and transmission. These results should be considered when predicting how anthropogenic food sources and shifts in natural food sources caused by climate change will affect wildlife epidemic dynamics.

We thank M. Sudnick and W. Kirkpatrick for their support in the lab.

Author contributions

Conceptualization: W.G.P., A.C.L., S.E.D.; Formal analysis: W.G.P., E.L.S., S.E.D.; Investigation: E.L.S., A.C.L., A.M., J.N., S.E.D.; Methodology: W.G.P., E.L.S., A.C.L., A.M., J.N., S.E.D.; Writing – original draft: W.G.P., E.L.S., S.E.D.; Writing – review & editing: W.G.P., E.L.S., A.C.L., S.E.D.

Funding

Funds were provided by grants to S.E.D. from the National Science Foundation (1941861) and Arkansas Biosciences Institute. Open Access funding provided by University of Arkansas. Deposited in PMC for immediate release.

Data availability

The data supporting the results are archived on GitHub: https://github.com/erinsauer/Perrine-et-al-MG-diet.

Adamo
,
S. A.
(
2006
).
Comparative psychoneuroimmunology: evidence from the insects
.
Behav. Cognit. Neurosci. Rev.
5
,
128
-
140
.
Adamo
,
S. A.
,
Roberts
,
J. L.
,
Easy
,
R. H.
and
Ross
,
N. W.
(
2008
).
Competition between immune function and lipid transport for the protein apolipophorin III leads to stress-induced immunosuppression in crickets
.
J. Exp. Biol.
211
,
531
-
538
.
Adelman
,
J. S.
and
Martin
,
L. B.
(
2009
).
Vertebrate sickness behaviors: adaptive and integrated neuroendocrine immune responses
.
Integr. Comp. Biol.
49
,
202
-
214
.
Adelman
,
J. S.
,
Carter
,
A. W.
,
Hopkins
,
W. A.
and
Hawley
,
D. M.
(
2013
).
Deposition of pathogenic Mycoplasma gallisepticum onto bird feeders: host pathology is more important than temperature-driven increases in food intake
.
Biol. Lett.
9
,
20130594
.
Amar
,
S.
,
Zhou
,
Q.
,
Shaik-Dasthagirisaheb
,
Y.
and
Leeman
,
S.
(
2007
).
Diet-induced obesity in mice causes changes in immune responses and bone loss manifested by bacterial challenge
.
Proc. Natl. Acad. Sci. USA
104
,
20466
-
20471
.
Barlein
,
F.
(
1990
).
Nutrition and food selection in migratory birds
. In
Bird Migration: Physiology and Ecophysiology
(ed.
E.
Gwinner
), pp.
1998
-
1213
.
Berlin
:
Springer
.
Becker
,
D. J.
,
Hall
,
R. J.
,
Forbes
,
K. M.
,
Plowright
,
R. K.
and
Altizer
,
S.
(
2018
).
Anthropogenic resource subsidies and host-parasite dynamics in wildlife
.
Philos. Trans. R. Soc. B
373
,
20170086
.
Behm
,
C. A.
and
Ovington
,
K. S.
(
2000
).
The role of eosinophils in parasitic helminth infections: insights from genetically modified mice
.
Parasitol. Today.
16
,
202
-
209
.
Bellrose
,
F. C.
and
Holm
,
D. J.
(
1994
).
Ecology and Management of the Wood Duck
.
Pennsylvania
:
Stackpole Books
.
Bouwman
,
K. M.
and
Hawley
,
D. M.
(
2010
).
Sickness behavior acting as an evolutionary trap? Male house finches preferentially feed near diseased conspecifics
.
Biol. Lett.
6
,
462
-
465
.
Cassat
,
J. E.
and
Skaar
,
E. P.
(
2013
).
Iron in infection and immunity
.
Cell Host Microbe
13
,
509
-
519
.
Coogan
,
S. C. P.
,
Raubenheimer
,
D.
,
Zantis
,
S. P.
and
Machovsky-Capuska
,
G. E.
(
2018
).
Multidimensional nutritional ecology and urban birds
.
Ecosphere
9
,
e02177
.
Cornet
,
S.
,
Bichet
,
C.
,
Larcombe
,
S.
,
Faivre
,
B.
and
Sorci
,
G.
(
2014
).
Impact of host nutritional status on infection dynamics and parasite virulence in a bird malaria system
.
J. Anim. Ecol.
83
,
256
-
265
.
Cotter
,
S. C.
,
Simpson
,
S. J.
,
Raubenheimer
,
D.
and
Wilson
,
K.
(
2011
).
Macronutrient balance mediates trade-offs between immune function and life history traits
.
Funct. Ecol.
25
,
186
-
198
.
Cressler
,
C. E.
,
Nelson
,
W. A.
,
Day
,
T.
and
McCauley
,
E.
(
2014
).
Disentangling the interaction among host resources, the immune system and pathogens
.
Ecol. Lett.
17
,
284
-
293
.
Cunningham-Rundles
,
S.
,
McNeeley
,
D. F.
and
Moon
,
A.
(
2005
).
Mechanisms of nutrient modulation of the immune response
.
J. Allergy Clin. Immunol.
115
,
1119
-
1128
.
Dhondt
,
A. A.
,
Dhondt
,
K. V.
,
Hawley
,
D. M.
and
Jennelle
,
C. S.
(
2007
).
Experimental evidence for transmission of Mycoplasma gallisepticum in house finches by fomites
.
Avian Pathol.
36
,
205
-
208
.
Dhondt
,
A. A.
,
Dhondt
,
K. V.
and
McCleery
,
B. V.
(
2008
).
Comparative infectiousness of three passerine bird species after experimental inoculation with Mycoplasma gallisepticum
.
Avian Pathol.
37
,
635
-
640
.
Dinarello
,
C. A.
(
1997
).
Role of pro-and anti-inflammatory cytokines during inflammation: experimental and clinical findings
.
J. Biol. Regul. Homeostat. Agents
11
,
91
-
103
.
Friedman
,
A.
and
Sklan
,
D.
(
1995
).
Effect of dietary fatty acids on antibody production and fatty acid composition of lymphoid organs in broiler chicks
.
Poult. Sci.
74
,
1463
-
1469
.
Gomes
,
S. V.
,
Dias
,
B. V.
,
Júnior
,
P. A. M.
,
Pereira
,
R. R.
,
de Souza
,
D. M. S.
,
Breguez
,
G. S.
,
de Lima
,
W. G.
,
Magalhães
,
C. L. D.
,
Cangussú
,
S. D.
,
Talvani
,
A.
et al.
(
2023
).
High-fat diet increases mortality and intensifies immunometabolic changes in septic mice
.
J. Nutr. Biochem.
116
,
109315
.
Graham
,
A. L.
,
Allen
,
J. E.
and
Read
,
A. F.
(
2005
).
Evolutionary causes and consequences of immunopathology
.
Annu. Rev. Ecol. Evol. Systemat.
36
,
373
-
397
.
Grieger
,
T. A.
and
Kluger
,
M. J.
(
1978
).
Fever and survival: the role of serum iron
.
J. Physiol.
279
,
187
-
196
.
Grodio
,
J. L.
,
Dhondt
,
K. V.
,
O'Connell
,
P. H.
and
Schat
,
K. A.
(
2008
).
Detection and quantification of Mycoplasma gallisepticum genome load in conjunctival samples of experimentally infected house finches (Carpodacus mexicanus) using real-time polymerase chain reaction
.
Avian Pathol.
37
,
385
-
391
.
Hall
,
R. J.
(
2019
).
Modeling the effects of resource-driven immune defense on parasite transmission in heterogenoeous host populations
.
Integr. Comp. Biol.
59
,
1253
-
1263
.
Hall
,
S. R.
,
Simonis
,
J. L.
,
Nisbet
,
R. M.
,
Tessier
,
A. J.
and
Cáceres
,
C. E.
(
2009
).
Resource ecology of virulence in a planktonic host-parasite system: an explanation using dynamic energy budgets
.
Am. Nat.
174
,
149
-
162
.
Hawley
,
D. M.
,
Grodio
,
J.
,
Frasca
,
S.
,
Kirkpatrick
,
L.
and
Ley
,
D. H.
(
2011
).
Experimental infection of domestic canaries (Serinus canaria domestica) with Mycoplasma gallisepticum: a new model system for a wildlife disease
.
Avian Pathol.
40
,
321
-
327
.
Hite
,
J. L.
and
Cressler
,
C. E.
(
2018
).
Resource-driven changes to host population stability alter the evolution of virulence and transmission
.
Philos. Trans. R. Soc. B
373
,
20170087
.
Hosseini
,
P. R.
,
Mills
,
J .N.
,
Prieur-Richard
,
A.
,
Ezenwa
,
V. O.
,
Bailly
,
X.
,
Rizzoli
,
A.
,
Suzan
,
G.
,
Vittecoq
,
M.
,
Garcia-Pena
,
G .E.
,
Daszak
,
P.
et al.
(
2017
).
Does the impact of biodiversity differ between emerging and endemic pathogens? The need to separate the concepts of hazard and risk
.
Philos. Trans. R. Soc. B
372
,
20160129
.
Huffman
,
M. A.
and
Seifu
,
M.
(
1989
).
Observations on the illness and consumption of a possibly medicinal plant Vernonia amygdalina (del.), by a wild chimpanzee in the Mahale Mountains National Park, Tanzania
.
Primates
30
,
51
-
63
.
Hutchings
,
M. R.
,
Athanasiadou
,
S.
,
Kyriazakis
,
I.
and
Gordon
,
I. J.
(
2003
).
Can animals use foraging behaviour to combat parasites?
Proc. Nutr. Soc.
62
,
361
-
370
.
Jahanian
,
R.
(
2009
).
Immunological responses as affected by dietary protein and arginine concentrations in starting broiler chicks
.
Poult. Sci.
88
,
1818
-
1824
.
Klasing
,
K. C.
(
1998
).
Comparative Avian Nutrition
.
CAB International
.
Klasing
,
K. C.
(
2007
).
Nutrition and the immune system
.
Brit. Poult. Sci.
48
,
525
-
537
.
Kluger
,
M. J.
and
Rothenburg
,
B. A.
(
1979
).
Fever and reduced iron: their interaction as a host defense response to bacterial infection
.
Science
203
,
374
-
376
.
Koch
,
R. E.
,
Kavazis
,
A. N.
,
Hasselquist
,
D.
,
Hood
,
W. R.
,
Zhang
,
Y.
,
Toomey
,
M. B.
and
Hill
,
G. E.
(
2018
).
No evidence that carotenoid pigments boost either immune or antioxidant defenses in a songbird
.
Nat. Commun.
9
,
491
.
Kurihara
,
T.
,
Warr
,
G.
,
Loy
,
J.
and
Bravo
,
R.
(
1997
).
Defects in macrophage recruitment and host defense in mice lacking the CCR2 chemokine receptor
.
J. Exp. Med.
186
,
1757
-
1762
.
LeBien
,
T. W.
and
Tedder
,
T. F.
(
2008
).
B Lymphocytes: how they develop and function
.
Blood
112
,
1570
-
1580
.
Lee
,
K. P.
,
Simpson
,
S. J.
and
Wilson
,
K.
(
2008
).
Dietary protein–quality influences melanization and immune function in an insect
.
Funct. Ecol.
22
,
1052
-
1061
.
Lin
,
B.
,
Qing
,
X.
,
Liao
,
J.
and
Zhuo
,
K.
(
2020
).
Role of protein glycosylation in host-pathogen interaction
.
Cells
9
,
1022
.
Long
,
G. H.
and
Graham
,
A. L.
(
2011
).
Consequences of immunopathology for pathogen virulence evolution and public health: malaria as a case study
.
Evol. Apps.
4
,
278
-
291
.
Love
,
A. C.
,
Grisham
,
K.
,
Krall
,
J. B.
,
Goodchild
,
C. G.
and
Durant
,
S. E.
(
2021
).
Perception of infection: disease-related social cues influence immunity in songbirds
.
Biol. Lett.
17
,
20210125
.
Love
,
A. C.
,
Anthony
,
A. C.
,
Nash
,
A.
,
Campos-Melara
,
A.
,
Kodali
,
J.
and
DuRant
,
S. E.
(
2023
).
Simulated infection alters the behavior of pair bonded songbirds and their healthy neighbors
.
Behav. Ecol.
34
,
251
-
260
.
Love
,
A. C.
,
Tabb
,
V.
,
Youssef
,
N. H.
,
Wilder
,
S. M.
and
Durant
,
S. E.
(
2024
).
Effect of dietary macronutrients and immune challenge on gut microbiota, physiology and feeding behaviour in zebra finches
.
Mol. Ecol.
33
,
e17428
.
Macpherson
,
A. J.
,
Hunziker
,
L.
,
McCoy
,
K.
and
Lamarre
,
A.
(
2001
).
IgA responses in the intestinal mucosa against pathogenic and non-pathogenic microorganisms
.
Microbes Infect.
3
,
1021
-
1035
.
Murray
,
M. J.
and
Murray
,
A. B.
(
1979
).
Anorexia of infection as a mechanism of host defense
.
Am. J. Clin. Nutr.
32
,
593
-
596
.
Murray
,
M. H.
,
Becker
,
D. J.
,
Hall
,
R. J.
and
Hernandez
,
S. M.
(
2016
).
Wildlife health and supplemental feeding: a review and management recommendations
.
Biological Cons.
204
,
163
-
174
.
Napier
,
B. A.
,
Andres-Terre
,
M.
,
Massis
,
L. M.
,
Hryckowian
,
A. J.
,
Higginbottom
,
S. K.
,
Cumnock
,
K.
,
Casey
,
K. M.
,
Haileselassie
,
B.
,
Lugo
,
K. A.
,
Schneider
,
D. S.
et al.
(
2019
).
Western diet regulates immune status and the response to LPS-driven sepsis independent of diet-associated microbiome
.
Proc. Natl. Acad. Sci. USA
116
,
3688
-
3694
.
Newton
,
I.
(
1967
).
The adaptive radiation and feeding ecology of some British Finches
.
Ibis
109
,
1
-
140
.
Nwaogu
,
C. J.
,
Galema
,
A.
,
Cresswell
,
W.
,
Dietz
,
M. W.
and
Tieleman
,
B. I.
(
2020
).
A fruit diet rather than invertebrate diet maintains a robust innate immunity in an omnivorous tropical songbird
.
J. Anim. Ecol.
89
,
867
-
883
.
Peng
,
L.
,
Yin
,
Y.-L.
,
Li
,
D.
,
Kim
,
S. W.
and
Wu
,
G.
(
2007
).
Amino acids and immune function
.
Brit. J. Nutr.
98
,
237
-
252
.
Pfenning-Butterworth
,
A. C.
,
Vetter
,
R. E.
and
Hite
,
J. L.
(
2023
).
Natural variation in host feeding behaviors impacts host disease and pathogen transmission potential
.
Ecol. Evol.
13
,
e9865
.
Pirola
,
L.
and
Ferraz
,
J. C.
(
2017
).
Role of pro-and anti-inflammatory phenomena in the physiopathology of type 2 diabetes and obesity
.
World J. Biol. Chem.
8
,
120
-
128
.
Plowright
,
R. K.
,
Foley
,
P.
,
Field
,
H. E.
,
Dobson
,
A. P.
,
Foley
,
J. E.
,
Eby
,
P.
and
Daszak
,
P.
(
2011
).
Urban habituation, ecological connectivity and epidemic dampening: the emergence of Hendra virus from flying foxes (Pteropus spp.)
.
Proc. R. Soc. B.
278
,
3703
-
3712
.
Povey
,
S.
,
Cotter
,
S. C.
,
Simpson
,
S. J.
,
Lee
,
K. P.
and
Wilson
,
K.
(
2009
).
Can the protein costs of bacterial resistance be offset by altered feeding behaviour?
J Anim. Ecol.
78
,
437
-
446
.
Povey
,
S.
,
Cotter
,
S. C.
,
Simpson
,
S. J.
,
Wilson
,
K.
and
Altizer
,
S.
(
2013
).
Dynamics of macronutrient self-medication and illness-induced anorexia in virally infected insects
.
J. Anim. Ecol.
83
,
245
-
255
.
Sauer
,
E. L.
,
Connelly
,
C.
,
Perrine
,
W.
,
Love
,
A. C.
and
Durant
,
S. E.
(
2024
).
Male pathology regardless of behaviour drives transmission in an avian host–pathogen system
.
J. Anim. Ecol.
93
,
36
-
44
.
Sieksmeyer
,
T.
,
He
,
S.
,
Esparza-Mora
,
M. A.
,
Jiang
,
S.
,
Petrašiūnaitė
,
V.
,
Kuropka
,
B.
,
Banasiak
,
R.
,
Julseth
,
M. J.
,
Weise
,
C.
,
Johnston
,
P. R.
et al.
(
2022
).
Eating in a losing cause: limited benefit of modified macronutrient consumption following infection in the oriental cockroach Blatta orientalis
.
BMC Ecol. Evol.
22
,
67
.
Sorci
,
G.
and
Faivre
,
B.
(
2009
).
Inflammation and oxidative stress in vertebrate host-parasite systems
.
Philos. Trans. R. Soc. B
364
,
71
-
83
.
Tschirren
,
B.
,
Bischoff
,
L. L.
,
Saladin
,
V.
and
Richner
,
H.
(
2007
).
Host condition and host immunity affect parasite fitness in a bird-ectoparasite system
.
Funct. Ecol.
21
,
372
-
378
.
Unckless
,
R. L.
,
Rottschaefer
,
S. M.
and
Lazzaro
,
B. P.
(
2015
).
The complex contributions of genetics and nutrition to immunity in Drosophila melanogaster
.
PLoS Genet.
11
,
e1005030
.

Competing interests

The authors declare no competing or financial interests.

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