ABSTRACT
Haemoglobin (Hb)–O2 binding affinity typically decreases with increasing temperature, but several species of ectothermic and regionally endothermic fishes exhibit reduced Hb thermal sensitivity. Regionally endothermic sharks, including the common thresher shark (Alopias vulpinus) and lamnid sharks such as the shortfin mako shark (Isurus oxyrinchus), can maintain select tissues and organs warmer than ambient temperature by retaining metabolic heat with vascular heat exchangers. In the ectothermic bigeye thresher shark (Alopias superciliosus), diurnal movements above and below the thermocline subject the tissues, including the blood, to a wide range of operating temperatures. Therefore, blood–O2 transport must occur across internal temperature gradients in regionally endothermic species, and over the range of environmental temperatures encountered by the ectothermic bigeye thresher shark. While previous studies have shown temperature-independent Hb–O2 affinity in lamnid sharks, including shortfin mako, the Hb–O2 affinity of the common and bigeye thresher sharks is unknown. Therefore, we examined the effect of temperature on whole-blood Hb–O2 affinity in common thresher shark and bigeye thresher shark. For comparison, analyses were also conducted on the shortfin mako shark and two ectothermic species, blue shark (Prionace glauca) and spiny dogfish (Squalus acanthias). Blood–O2 binding affinity was temperature independent for common thresher shark and shortfin mako shark, which should prevent internal temperature gradients from negatively affecting blood–O2 transport. Blue shark and spiny dogfish blood–O2 affinity decreased with increasing temperature, as expected, but bigeye thresher shark blood exhibited both a reduced temperature dependence and a high Hb–O2 affinity, which likely prevents large changes in environment temperature and low environmental oxygen from affecting O2 uptake.
INTRODUCTION
Like many highly migratory marine species, sharks in the families Lamnidae (i.e. mackerel sharks) and Alopiidae (i.e. thresher sharks) encounter a wide range of environmental temperatures during vertical movements through the water column or over long latitudinal migrations (e.g. Bernal et al., 2009; Sepulveda et al., 2019; Weng et al., 2005). For example, shortfin mako sharks (Isurus oxyrinchus) and common thresher sharks (Alopias vulpinus) periodically descend below the thermocline into water that may be more than 10°C cooler than surface waters (Cartamil et al., 2011, 2016; Holts and Bedford, 1993; Sepulveda et al., 2004). Similarly, bigeye thresher sharks (Alopias superciliosus) also experience particularly large and rapid changes in environmental temperature as they spend most of the day in deep cold water (<10°C) proximal to the upper reaches of the oxygen minimum layer, but during the night they ascend into the warmer mixed layer (>20°C) (Coelho et al., 2015; Nakano et al., 2003; Sepulveda et al., 2019; Weng and Block, 2004). During these diurnal movements above and below the thermocline, bigeye thresher shark body temperature closely tracks environmental temperature, which subjects the muscles and organs, including the blood, to a wide range of operating temperatures (e.g. 6–25°C; Aalbers et al., 2021; Sepulveda et al., 2019). Because the body temperature of most fishes mirrors that of the surrounding water temperature (Carey et al., 1971), and temperature affects the O2 affinity of blood (reviewed by Weber and Campbell, 2011), moving between disparate thermal environments causes temporal changes in body temperature that have important implications for the transport of O2 from the water to the tissues (e.g. Carey, 1982).
Among jawed vertebrates, haemoglobin (Hb)–O2 binding affinity generally decreases with increasing blood temperature because O2 binding to the haem groups is exothermic (Barcroft and Hill, 1910). This effect of temperature on Hb–O2 affinity is indicated by overall values for the enthalpy change of oxygenation that are negative (i.e. exothermic) (reviewed by Weber and Campbell, 2011). However, Atlantic bluefin tuna (Thunnus thynnus) Hb–O2 binding affinity was shown to be insensitive to temperature (Rossi-Fanelli and Antonini, 1960), even exhibiting a reverse temperature dependence (i.e. ΔH′ is positive and Hb–O2 affinity increases with increasing temperature) (Carey and Gibson, 1977; but also see Brill and Bushnell, 2006), and reduced and reversed temperature dependence has been reported in several other tuna (Scombridae) (Brill and Bushnell, 1991; Cech et al., 1984; Clark et al., 2008; Lilly et al., 2015; Sharp, 1975). Lamnid sharks also have Hbs with reduced or reversed temperature dependence (Andersen et al., 1973) and, like tunas, they have evolved the ability to maintain distinct regions of their bodies warmer than the surrounding water (regional endothermy) (Bernal et al., 2001; Carey and Teal, 1966, 1969; Carey et al., 1971, 1985). The common thresher shark is also regionally endothermic (Bernal and Sepulveda, 2005), and although the thermal sensitivity of common thresher shark Hb is unknown, reduced and reverse temperature-dependent Hb–O2 affinity in tunas and lamnid sharks has been linked to the oxygenation-dependent dissociation of allosteric effectors (e.g. protons and organic phosphates), which contribute endothermically to ΔH′ (Ikeda-Saito et al., 1983; Larsen et al., 2003; Weber and Campbell, 2011).
Regionally endothermic sharks can retain the metabolic heat produced by their red, slow-twitch, swimming muscles (RM) with vascular heat exchangers (i.e. retia mirabilia), which enable RM temperatures to be elevated by 4–10°C above ambient temperature in the shortfin mako shark and the common thresher shark (Bernal and Sepulveda, 2005; Carey and Teal, 1969; Carey et al., 1985; Patterson et al., 2011). In contrast, ectothermic fishes thermoconform with their environment, in large part as a result of the convective transfer of metabolic heat in the blood to the gill circulation where heat is lost to the inspired water (Stevens and Sutterlin, 1976). Like tunas, the RM of regionally endothermic sharks is deeply set in the body compared with the superficial position of the RM in most other fishes (Bernal et al., 2001; Carey and Teal, 1966, 1985; Sepulveda et al., 2005). The bulk of the arterial blood flow entering the systemic circulation is directed laterally through subcutaneous arteries and veins, which redirects the blood supply to the medially situated RM through heat exchanging retia, where the cold arterial blood is warmed before it perfuses the RM (Carey and Teal, 1969; Carey et al., 1985; Patterson et al., 2011). The retia enable heat transfer to the arterial blood from the warmer venous blood exiting the metabolically active (i.e. contracting) RM, efficiently conserving heat and cooling the outflowing venous blood near to ambient temperature before it reaches the gills. Consequently, blood flowing through a heat exchanging rete is subjected to what has been described as ‘closed-system’ warming and cooling, as the change in blood temperature will directly affect the partial pressures of oxygen (PO2) and carbon dioxide (PCO2), but the content of blood gases will remain relatively constant as the rete vessels (i.e. arterioles and venules) should preclude diffusion of gases out of the blood (Brill and Bushnell, 1991; Cech et al., 1984; Stevens et al., 1974). Accordingly, it has also been proposed that temperature-independent Hb–O2 affinity may avert premature Hb–O2 unloading as the blood is warmed in transit from the gills to the warmer tissues of regionally endothermic fishes (Carey and Gibson, 1977; Graham, 1973).
While there are three extant species of thresher shark – the common thresher shark, the bigeye thresher shark and the pelagic thresher shark (Alopias pelagicus) – only the common thresher shark is a known regional endotherm (Bernal and Sepulveda, 2005; Carey et al., 1971; Patterson et al., 2011). As in other ectothermic sharks, the RM is positioned superficially in the bigeye and pelagic thresher sharks, and RM temperature does not differ from that of the surrounding water (Patterson et al., 2011; Sepulveda et al., 2005). While it has been speculated that the bigeye thresher shark has warm eyes (cranial endothermy) because the orbital circulation is enlarged, suggestive of cranial endothermy (Block and Carey, 1985; Weng and Block, 2004), preliminary data on cranial temperature measurements obtained for bigeye thresher sharks suggest that the temperature of these tissues does not differ significantly from that of the white muscle (D.B. and C.A.S., unpublished). However, bigeye thresher sharks do exhibit extreme temperature tolerance, transitioning between warm surface waters (∼18–25°C) and colder water below the thermocline (<10°C), and remain in these disparate thermal environments for extended periods (10–12 h) (Aalbers et al., 2021; Nakano et al., 2003; Sepulveda et al., 2019; Weng and Block, 2004). Thus, Hb–O2 transport must occur over the range of environmental temperatures encountered by the ectothermic bigeye thresher shark, and these daily temperature changes may potentially impair O2 transport unless the temperature dependence of Hb–O2 affinity is reduced, which we propose here.
We are not aware of any published studies on Hb–O2 binding affinity in any thresher shark species, so this was the drive behind our own investigation. Previous studies have shown reduced Hb thermal sensitivity in at least three lamnid sharks (regional endotherms): the shortfin mako shark, the porbeagle shark (Lamna nasus) and the salmon shark (Lamna ditropis) (Andersen et al., 1973; Bernal et al., 2018; Dickinson and Gibson, 1981). We therefore hypothesized analogous reduced Hb thermal sensitivity in common thresher shark. Furthermore, as the bigeye thresher shark remains poorly studied and exhibits extreme fluctuations in ambient daily temperatures, we also hypothesized that bigeye thresher shark Hb has a reduced temperature dependence. Bigeye thresher sharks spend most of the day proximal to the upper reaches of the oxygen minimum layer (Aalbers et al., 2021; Sepulveda et al., 2019), so we also hypothesized that this species has a high Hb–O2 affinity to extract oxygen from the relatively hypoxic water. We also conducted experiments on blood from shortfin mako sharks to corroborate and increase the sample size from Bernal et al. (2018). Finally, to corroborate our findings with a comparative approach, analyses were also conducted on blood from two previously studied ectothermic sharks, the blue shark (Prionace glauca) and the spiny dogfish (Squalus acanthias) (Bernal et al., 2018; Wells and Weber, 1983). The blue shark is pelagic and occupies an ecological niche that overlaps with those of the shortfin mako, common thresher and bigeye thresher sharks (i.e. sympatric), while the spiny dogfish is generally a temperate-coastal species but sometimes encounters variable depths (generally <600 m) and water temperatures (∼5–15°C) (Bernal et al., 2009; Sulikowski et al., 2010). We assessed the temperature sensitivity of whole blood from these sharks by constructing oxygen equilibrium curves (OECs; the relationship between PO2 and Hb–O2 saturation) and quantifying P50 (the PO2 at 50% Hb–O2 saturation) at different temperatures, as well as by measuring the PO2 while the blood was warmed or cooled in an experimental closed-system meant to mimic the temperature changes that the blood experiences in the arterioles and venules of a heat exchanging rete.
MATERIALS AND METHODS
All capture, handling and experimental procedures followed guidelines approved by the University of Massachusetts (animal care protocol no. 13-06), the California Department of Fish and Wildlife (Scientific Collection permit nos. SC-2471, SC-12372) and the University of British Columbia (UBC) Animal Care Committee (animal care no. A11-0235 and A15-0266). All partial pressure and P50 values are reported in mmHg (1 mmHg=0.133 kPa).
Blood collection
Bigeye thresher sharks, Alopias superciliosus Lowe 1841 (n=9), were captured by deep-set buoy gear (Sepulveda et al., 2014, 2019) in the coastal waters off Southern California (i.e. the Southern California Bight). Shortfin mako, Isurus oxyrinchus Rafinesque 1810 (n=7), and blue sharks, Prionace glauca (Linnaeus 1758) (n=5), were captured by hook and line off the coast of Southern California, and common thresher sharks, Alopias vulpinus (Bonnaterre 1788) (n=2) and spiny dogfish, Squalus acanthias Linnaeus 1758 (n=8), were captured by hook and line off the coast of Massachusetts (see Table 1 for fork lengths). Individuals were either restrained alongside the research vessel or quickly brought aboard and restrained. Blood was then withdrawn by caudal puncture using heparinized syringes, a method previously used for obtaining shark blood for oxygen equilibria experiments (Bernal et al., 2018; Brill et al., 2008; Cooper and Morris, 1998). All sharks were released after sampling. Blood samples were kept on ice and overnight shipped by courier to the University of British Columbia (UBC), Vancouver, Canada, where the blood was stored at 4°C. All experiments on whole blood were conducted within 1–4 days post-capture. This sampling technique has been successfully employed for previous studies on both teleosts and sharks (Bouyoucos et al., 2020; Clark et al., 2010; Morrison et al., 2022; Polinski et al., 2021; Zhang et al., 2019). However, during this time, red blood cell (RBC) intracellular nucleoside triphosphate (NTP) levels likely decreased relative to in vivo levels, possibly causing higher blood–O2 affinity with altered temperature sensitivity versus that in freshly sampled blood. Although we were not able to construct OECs on freshly drawn blood, we have shown that swordfish (Xiphias gladius) blood P50 was relatively stable from 4 to 8 days post-collection (Morrison et al., 2022). In another previous study conducted at the UBC Vancouver campus using blood collected from chub mackerel (Scomber japonicus) off the coast of Southern California, it was concluded that blood was viable for up to 6 days when stored at 4°C (Clark et al., 2010). Possible shortfalls of this study are further discussed below.
Haematological parameters
Immediately after blood samples arrived at UBC, Hb concentration and haematocrit (Hct) were measured, and subsamples of blood were centrifuged to separate the plasma from the RBCs for measurement of plasma osmolality. The remaining plasma was frozen at −80°C for determination of plasma lactate concentration. Hb concentration, expressed as tetrameric Hb ([Hb4], in mmol l−1) was measured by the cyanmethaemoglobin method using Drabkin's reagent and a haem-based extinction coefficient of 11 mmol−1 cm−1 at a wavelength of 540 nm (e.g. Völkel and Berenbrink, 2000). Hct was measured as the percentage of packed RBCs relative to total blood volume after centrifuging samples in glass microcapillary tubes at approximately 13,000 RCF for 5 min. Mean corpuscular haemoglobin concentration (MCHC, in mmol l−1) was calculated as [Hb4]/(Hct/100). Plasma osmolality (mOsm kg−1) was measured in 10 µl of undiluted plasma with a vapour pressure osmometer (VAPRO 5520, Wescor, Logan, UT, USA). Plasma lactate was measured spectrophotometrically using the LDH-catalysed reaction converting lactate to pyruvate, where the reduction of NAD+ to NADH was measured at 340 nm (Bergmeyer et al., 1983).
Whole-blood oxygen equilibria, pH and PO2
Whole-blood OECs were constructed by quantifying the relative Hb–O2 saturation over a range of equilibration PO2 values at two physiologically relevant carbon dioxide levels, low (1.9 mmHg/0.25%) and high (3.8 mmHg/0.50% for spiny dogfish and 7.6 mmHg/1.00% for all others), and at two temperatures, 10 and 25°C for bigeye thresher shark, 15 and 22°C for common thresher shark, and 15 and 25°C for shortfin mako shark, blue shark and spiny dogfish. The colder experimental temperatures (10 or 15°C) are within the range of the colder water temperatures regularly encountered by these species (Carey et al., 1990; Cartamil et al., 2016; Sepulveda et al., 2004, 2019; Sulikowski et al., 2010). The warmest experimental temperatures corresponded to the warmest water temperatures encountered by bigeye thresher sharks and blue sharks (25°C), and the warmest RM temperatures in the mako (25°C) and the common thresher sharks (22°C) (Bernal and Sepulveda, 2005; Carey et al., 1990; Carey and Teal, 1969; Patterson et al., 2011; Sepulveda et al., 2019). Spiny dogfish rarely encounter water as warm as 25°C, but constructing OECs at 25°C allowed direct comparison with the other species included in this study.
The relationship between Hb–O2 saturation and PO2 (i.e. an OEC) was assessed on two to three replicate samples using a custom microplate-based, parallel assay, multi-cuvette tonometry cell as described by Lilly et al. (2013), and following the procedure outlined in Morrison et al. (2022). Cuvettes were formed by sandwiching blood samples (∼3–5 μl) between two sheets of low-density polyethylene (Glad® ClingWrap) that were secured on an aluminium ring with two plastic O-rings, which were then placed in a gas-tight tonometry cell designed to fit into a SpectraMax 190 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Experimental temperatures were achieved by placing the microplate reader in a temperature-controlled environmental chamber. Optical density (OD) was measured every 20–30 s at 390 nm (near an isosbestic point between oxygenated and deoxygenated Hb, where OD is independent of Hb–O2 saturation), and at 430 nm and 436 nm (near the peak absorption for deoxygenated Hb), wavelengths commonly used in thin-film optical methods for measuring Hb–O2 saturation (e.g. Clark et al., 2008; Reeves, 1980; Weber et al., 2010). Initially, blood was equilibrated with pure N2 for a minimum of 30 min until OD at 430 and 436 nm was stable, which was assumed to indicate full Hb deoxygenation. After deoxygenation, the Hb–O2 saturation was increased with at least nine stepwise increments of the O2 tension, balanced with N2, up to a PO2 of 159.6 mmHg (i.e. 21% O2, an approximation of the PO2 in dry atmospheric air at sea level). Full Hb–O2 saturation was assumed after a final increment to a PO2 of 228 mmHg in the absence of CO2. Dry gas mixtures of O2, CO2 and N2 (>99.5% pure medical gases; Linde Canada Inc.) were obtained using a DIGAMIX® gas mixing pump (H. Wösthoff Messtechnik, Bochum, Germany), and gases were not humidified as the blood samples were sealed in plastic wrap. OEC experiments lasted between 1 and 2 h, and in preliminary experiments no significant methaemoglobin (MetHb) formation occurred during the experiments. MetHb was qualitatively assessed by recording absorption spectra following the final oxygenation step, and the spectra showed no unusually high absorbance at 630 nm, an absorption maximum for metHb (e.g. Völkel and Berenbrink, 2000; Zijlstra and Buursma, 1997). Therefore, we conclude that metHb formation did not significantly affect our Hb–O2 saturation measurements in the remainder of the experiments. At each equilibration step, the difference in OD (ΔOD) between 390 nm and 430 nm or between 390 nm and 436 nm (ΔOD=OD 430 nm−OD 390 nm or OD 436 nm−OD 390 nm) was used to calculate the fractional Hb–O2 saturation ([Hb–O2]/[Hb]) from the change in ΔOD from full deoxygenation, relative to that between full deoxygenation (pure N2) and full oxygenation (PO2 of 228 mmHg and no CO2). OECs constructed using OD at 430 nm were identical to those constructed using OD at 436 nm.
Blood pH was measured in approximately 500 μl subsamples of blood equilibrated for 1 h with either the low or high CO2 tension, and a range of O2 tensions between 7.6 and 159.6 mmHg (balanced with N2) in rotating glass tonometers thermostatically set to 10, 15, 22 or 25°C. The gas mixtures were humidified at the experimental temperature prior to entering the tonometers. Blood was drawn into a gas-tight syringe pre-flushed with the gas mixture, and pH was measured by drawing the blood through a pH electrode (16-705 flow-thru pH electrode, Microelectrodes Inc., Bedford, NH, USA) in combination with a 16-702 flow-thru reference electrode (Microelectrodes Inc.) thermostatically set to the experimental temperature.
Closed-system temperature changes
To mimic the closed-system temperature changes that blood experiences in the arterioles and venules of a heat exchanging rete, approximately 500 μl blood samples equilibrated at 10, 15, 22 or 25°C were injected into a pH electrode (as described above) and a Radiometer E5046 PO2 electrode thermostatically set to the equilibration temperature as well as another pair of electrodes thermostatically set to a warmer or cooler experimental temperature according to Cech et al. (1984), Brill and Bushnell (1991) and Bernal et al. (2018). Bigeye thresher shark blood temperature was changed between 10 and 25°C, common thresher shark blood temperature was changed between 10 and 22°C, and shortfin mako shark, blue shark and spiny dogfish blood temperature was changed between 15 and 25°C. Although the blood was static within the electrode chamber, it was rapidly heated or cooled in a system where there is minimal exchange of gases and ions between the blood and another medium. Prior to blood injection, each PO2 electrode was flushed with the experimental gas mixture to minimize electrode response time to the respective PO2. Temperature-induced changes in pH and PO2 were monitored using Acqknowledge® Data Acquisition Software (version 3.7.3, BIOPAC Systems, Inc.) by viewing traces of pH and PO2, and when it appeared that the traces had stabilized, the respective values of each were recorded.
Data analysis
[Hb], Hct and MCHC were compared among species by one-way ANOVA, and differences among the species means were assessed by Tukey's multiple comparison test using GraphPad Prism version 6.01 (GraphPad Software, La Jolla, CA, USA). All other statistical analyses, curve fitting and linear mixed model fitting were performed in R v 4.1.3 (http://www.R-project.org/).
RESULTS
Blood parameters and species lengths are summarized in Table 1. Mean [Hb] and Hct values for shortfin mako sharks were significantly greater than those of bigeye thresher sharks, and while [Hb] and Hct were not different between blue sharks and spiny dogfish, they were lower than those of shortfin mako sharks and bigeye thresher sharks (Hb: F3,26=40.60, P<0.0001; Hct: F3,26=26.74, P<0.0001). MCHC was not different between shortfin makos and bigeye threshers, which both had higher MCHC than blue sharks and spiny dogfish (F3,26=16.30, P<0.0001). Common thresher shark haematological values were not included in the analyses as only two individuals were sampled, but [Hb], Hct and MCHC for the two common thresher sharks were within the range of values measured in shortfin makos (Table 1).
Whole-blood experiments
Whole-blood OECs were successfully constructed for seven shortfin mako sharks, two common thresher sharks, five bigeye thresher sharks, four blue sharks and seven spiny dogfish. Measured whole-blood OECs are shown in Fig. 1, the effect of temperature and pH, and blood–O2 affinity (P20, P50 and P80) are shown in Bohr plots in Fig. 2, and modelled OECs at different temperatures and pH are shown in Fig. 3. The range of blood pH levels measured in the whole-blood OEC experiments is reported in Table 2 for each species, along with P50 values, nH values and Bohr coefficients. Blood P50 values for the shortfin mako shark, the bigeye thresher shark and the blue shark were predicted at pH 7.7 and pH 7.5, which are approximated arterial pH values for the blue shark and the mako shark, respectively (Lai et al., 1997). Spiny dogfish P50 values are reported at an arterial pH of 7.85 (Swenson and Maren, 1987; Wells and Weber, 1983), as well as pH 7.7 for comparison among species. Common thresher shark P50 values are reported at pH 7.3 because of the relatively acidotic state of the blood from the two individuals that were sampled (Fig. 3C and Table 2). Blood pH and PCO2 had little influence on P50 for all species, as indicated by low Bohr coefficients and 95% CIs that included zero (Table 2).
Temperature was not a significant predictor of blood PO2 at any saturation level in blood of both shortfin mako shark and common thresher shark. In bigeye thresher shark blood, temperature was not a significant predictor of blood PO2 at or below 60% saturation, but from 70% to 95% saturation, temperature was an important model factor (χ2=8.052−26.321, d.f.=2, P≤0.018), where increasing temperature decreased blood–O2 affinity. Blood–O2 affinity was significantly decreased by increasing temperature for both blue shark and spiny dogfish, and temperature was an important predictor of blood PO2 at all saturations (blue shark: χ2=8.481−41.751, d.f.=2, P≤0.014; spiny dogfish: χ2=39.488−54.670, d.f.=2, P<0.001).
Hill coefficients were not significantly influenced by blood pH or temperature for any species except the bigeye thresher shark (Fig. 2). Temperature was an important predictor of bigeye thresher nH values (χ2=19.847, d.f.=2, P=0.000049), with lower values at 25°C than at 10°C, and pH was an important predictor of bigeye thresher nH values at 10°C (β=1.034, P=0.017) but not at 25°C. Shortfin mako shark nH values ranged from 1.25 to 2.91 at 15°C and 1.44 to 2.20 at 25°C, common thresher shark nH values ranged from 1.12 to 1.63 at 15°C and 1.10 to 1.73 at 22°C, bigeye thresher shark nH values ranged from 1.52 to 2.28 at 10°C and 1.07 to 1.77 at 25°C, spiny dogfish nH values ranged from 0.91 to 1.17 at 15°C and 1.03 to 1.67 at 25°C, and blue shark nH values ranged from 1.27 to 1.47 at 10°C, 1.13 to 1.79 at 15°C, and 0.89 to 1.39 at 25°C (Fig. 2).
The effects of closed-system temperature changes on blood PO2 are shown in Fig. 4, with predicted temperature-induced changes in plasma PO2 following Henry's law (i.e. the temperature dependence of plasma O2 solubility will cause PO2 in a closed system to increase or decrease by increasing or decreasing temperature, respectively) using O2 solubilities from Boutilier et al. (1984). Closed-system warming and cooling of shortfin mako and common thresher shark blood generally increased and decreased blood PO2, respectively, but the change in PO2 (ΔPO2) was less than the predicted change. Closed-system warming and cooling of blood from bigeye thresher sharks, blue sharks and spiny dogfish changed PO2 beyond that predicted by the change in solubility of O2, presumably as a result of temperature induced Hb–O2 offloading and binding with warming and cooling, respectively. The greatest ΔPO2 occurred in bigeye thresher shark blood (Fig. 4D).
DISCUSSION
Our primary objective was to compare the effect of temperature on whole-blood Hb–O2 binding affinity in two closely related sharks that experience either frequent and large temporal changes in body temperature (bigeye thresher shark) or are capable of regional endothermy (common thresher shark). We also measured the effect of temperature on blood–O2 affinity in shortfin mako shark, blue shark and spiny dogfish using comparable methods to those used for thresher sharks. This study tested the hypothesis that blood–O2 affinity in both the common thresher shark and the bigeye thresher shark is less affected by temperature than in most ectothermic species, similar to the blood and Hbs of regionally endothermic lamnid sharks (i.e. the shortfin mako shark, the porbeagle and the salmon shark) (Andersen et al., 1973; Bernal et al., 2018; Dickinson and Gibson, 1981; Larsen et al., 2003). Additionally, this study also tested the hypothesis that the bigeye thresher shark has a relatively high blood–O2 affinity as this species encounters low environmental oxygen levels, daily. We observed that whole-blood Hb–O2 affinity was independent of temperature for the regionally endothermic common thresher shark (Figs 2C and 3B) and shortfin mako shark (Figs 2A and 3A), whereas bigeye thresher shark blood exhibited a temperature dependence that was dependent on Hb–O2 saturation (Figs 2G and 3D). Bigeye thresher shark blood also had a high O2 affinity with a P50 around 8 mmHg at 10 and 25°C, which is less than half the P50 for the common thresher shark, blue shark and spiny dogfish at 25°C (Table 2).
Justification of blood sampling methodology
To obtain blood samples for this study, blood was taken from sharks shortly after capture at sea, as it is impracticable and unsafe to obtain blood samples from resting and cannulated large sharks held in laboratory aquaria. The individuals included in this study had likely experienced varying levels of fatigue and consequent respiratory and metabolic acidosis. This was indicated by relatively high plasma lactate levels (Table 1; mean±s.e.m.) in shortfin mako sharks (8.8±4.2 mmol l−1), common thresher sharks (7.1 mmol l−1) and blue sharks (7.2±2.0 mmol l−1); however, these mean values are lower than mean lactate levels reported from capture-stressed shortfin mako sharks (13–16 mmol l−1) and blue sharks (9 mmol l−1) (Wells and Davie, 1985; Wells et al., 1986). Spiny dogfish plasma lactate levels (1.4±0.2 mmol l−1) were similar to resting levels of 1 mmol l−1 (Richards et al., 2003), while bigeye thresher shark plasma lactate levels (3.4±0.35 mmol l−1) were similar to levels measured in capture-stressed sandbar sharks (Carcharhinus plumbeus; ∼4 mmol l−1; Brill et al., 2008), and were intermediate between those for spiny dogfish and those for shortfin mako, common thresher and blue sharks. Except for the two common thresher sharks, the blood pH levels that we achieved with the CO2 exposures (Tables 1 and 2) were within the range of arterial and venous blood pH levels measured in resting or slowly swimming sharks (∼pH 7.9–7.3; reviewed by Morrison et al., 2015), which allowed us to construct OECs within a physiologically relevant pH range (pH 7.7–7.3; Fig. 3). Although pH was low in common thresher shark blood, the blood showed no signs of RBC lysis and Hct was close to previously published values (Emery, 1986; Filho et al., 1992a). Hct, [Hb] and MCHC values for the other species included in this study (Table 1) were also within the range of previously published values for shortfin mako, blue shark and spiny dogfish (reviewed by Morrison et al., 2015).
In this study, we did not measure the RBC intracellular concentrations of NTPs such as adenosine triphosphate (ATP) and guanosine triphosphate (GTP), which can affect Hb–O2 affinity and its dependence on temperature and pH (Larsen et al., 2003; Morrison et al., 2015). Strenuous swimming associated with capture stress can cause RBC NTP levels to decrease (Brill et al., 2008), and NTP levels can also decline over time after blood withdrawal, which both will cause the ratio of RBC NTP concentration to MCHC (NTP/Hb) to decline and Hb–O2 affinity to potentially increase (i.e. lower P50 at lower NTP levels). Therefore, RBC NTP levels may be higher in fresh blood from resting sharks than in blood withdrawn from capture-stressed sharks and stored in a refrigerator for several days, potentially causing lower binding affinities with different temperature dependencies from those that we measured. However, NTP/Hb ratios for sharks are generally lower and relatively stable compared with those of teleosts, and mean NTP/Hb ratios of capture-stressed shortfin mako (0.3–0.7) and blue sharks (0.5) were within the range of ratios reported for unstressed elasmobranchs (0.3–1.6) (Filho et al., 1992b; Morrison et al., 2015; Wells and Davie, 1985; Wells et al., 1986).
Lastly, although only two common thresher sharks were included in this study, the results of the whole-blood experiments were consistent between the two individuals. Furthermore, our results on the effect of temperature on shortfin mako shark blood–O2 affinity (n=7) are consistent with the limited sample size (n=3) of Bernal et al. (2018). This evidence is suggestive that the common thresher shark data are representative of the species.
Temperature dependence of Hb–O2 affinity
Temperature had little to no effect on blood P50 of the two regionally endothermic species, shortfin mako shark and common thresher shark, and the blood P50 of the ectothermic bigeye thresher shark was also not affected by temperature. In contrast, increasing temperature increased P50 (i.e. decreased blood–O2 affinity) in blue shark and spiny dogfish blood (Table 2), and as expected for ectotherms, the temperature dependence of blood–O2 affinity was consistent across most of the OEC (i.e. at all saturation levels; Fig. 3). However, temperature did not uniformly affect the OEC of the bigeye thresher shark, which is also an ectotherm. At low Hb–O2 saturation, bigeye thresher shark blood–O2 affinity was independent of temperature, but above 60% saturation, O2 affinity decreased with increasing temperature (Fig. 3D).
Temperature-dependent binding of allosteric effectors such as ATP and H+ ions (Bohr protons) has been proposed to underlie reduced and reverse temperature-dependent Hb–O2 affinity among regionally endothermic fishes (Ikeda-Saito et al., 1983; Larsen et al., 2003; Morrison et al., 2022; Weber and Campbell, 2011; Weber et al., 2010). The major Hb components from the porbeagle have high intrinsic O2 affinities with a normal temperature dependence, but the addition of ATP to stripped Hb reduces O2 affinity and reverses the effect of temperature, as a result of oxygenation-linked dissociation of ATP and protons that contribute endothermically to ΔH′, causing it to become positive (Larsen et al., 2003). It is not yet clear what underlies temperature-independent Hb–O2 affinity in other lamnid sharks and the thresher sharks, but the molecular and structural underpinnings of reduced thermally sensitive Hbs in lamnid and alopiid is an area worthy of additional research.
Closed-system temperature changes
Temperature-independent Hb–O2 affinity in blood from the shortfin mako shark and the common thresher shark was reflected in closed-system changes to blood temperature, when blood PO2 changed less than would be expected due to the temperature dependence of plasma O2 solubility (Fig. 4A,B). Similar results have been previously reported for the shortfin mako shark, and tunas with Hbs that exhibit temperature-independent or reverse temperature-dependent O2 affinity (Bernal et al., 2018; Brill and Bushnell, 1991, 2006; Cech et al., 1984). In comparison, closed-system temperature changes caused large changes to blood PO2 for the ectothermic species included in this study – bigeye thresher shark, blue shark and spiny dogfish (Fig. 4C–E) – presumably indicating that warming and cooling of blood caused Hb–O2 unloading and binding, respectively. In bigeye thresher shark blood, the magnitude of temperature-induced changes to blood PO2 exceeded those measured in blue shark and spiny dogfish blood. Most of the equilibration O2 tensions used in this study greatly exceeded the bigeye thresher shark P50 of 8 mmHg, so the closed-system temperature changes likely occurred at high Hb–O2 saturation levels where temperature has the greatest influence on the bigeye thresher shark OEC (Fig. 3D), probably causing excessive Hb–O2 dissociation and binding as the blood is warmed and cooled, respectively.
Blood–O2 carrying capacity, blood–O2 affinity and low environmental O2
Shortfin mako shark and common thresher shark Hct and [Hb] are higher than typical values for ectothermic sharks, indicating a higher blood–O2 carrying capacity that is consistent with previous studies of sharks capable of red muscle endothermy (Bernal et al., 2001; Emery, 1986). The Hct and [Hb] for bigeye thresher shark are similar to those of hammerhead sharks in the genus Sphyrna, which are intermediate between typical values for other ectothermic sharks and those for regionally endothermic sharks (reviewed by Morrison et al., 2015). This may indicate that bigeye thresher sharks as well as some other large pelagic sharks have higher oxygen demands (i.e. higher active metabolic rates) than most other ectothermic sharks, so they potentially have relatively higher blood-O2 carrying capacities to match.
This study's whole-blood–O2 equilibria results for shortfin mako shark and blue shark blood are qualitatively like those reported by Bernal et al. (2018). As in this study, Bernal et al. (2018) also observed a reduced effect of temperature on shortfin mako shark blood–O2 affinity, and a normal decrease in blood–O2 affinity with increasing temperature in blue shark blood. However, their shortfin mako shark P50 values (14.5–22.3 mmHg) and Bohr coefficients (ϕ=−0.11 to −0.74) are larger than those reported here (Table 3). These discrepancies may be due to potentially low RBC NTP levels in this study compared with the relatively fresher blood samples used by Bernal et al. (2018), although the lower P50 value (10.6 mmHg at pH 7.6) and small Bohr coefficient (φ=0.16) reported by Wells and Davie (1985) for shortfin mako shark blood at 25°C (Table 3) are almost identical to those of this study at the same temperature and pH (P50=10.8 mmHg and φ=0.02; Table 2). Blue shark P50 values at 25°C are about 3–5 mmHg lower in this study than in that of Bernal et al. (2018) (Tables 2 and 3), which may be due to potentially lower RBC NTP levels in this study, but at 15°C P50 values were similar between the studies, and the slightly larger Bohr coefficients that Bernal et al. (2018) reported at both 15 and 25°C (Table 3) fall within the 95% CIs for the Bohr coefficients reported here (Table 2). The spiny dogfish P50 value reported by Wells and Weber (1983) at 15°C and pH 7.85 (17.9 mmHg) is slightly lower than the value that we determined (22.8 mmHg), and they reported a Bohr coefficient of −0.28 (Table 3), whereas we observed no significant effect of pH on P50 at 15°C (Table 2).
The bigeye thresher shark whole-blood P50 values are among the lowest reported for any elasmobranch, and they are similar to those of some hypoxia-tolerant freshwater teleosts (Harter et al., 2022; Morrison et al., 2015). Although it is possible that the low P50 values may be partly due to low RBC NTP levels associated with capture stress and blood storage duration (as discussed above), we suspect that such a low P50 that is temperature independent likely benefits O2 uptake over the range of environmental conditions that bigeye thresher sharks encounter, as a consequence of the vastly different daytime and nocturnal distributions. Bigeye thresher sharks spend most of the night in the warmer upper mixed layer (∼7 mg O2 l−1; PO2>150 mmHg), but during the day they descend well below the thermocline, proximal to the upper reaches of the oxygen minimum layer (<2 mg O2 l−1; PO2<34 mmHg) (Aalbers et al., 2021; Sepulveda et al., 2019; oxygen tensions determined with depth, temperature and dissolved oxygen concentrations reported by Sepulveda et al., 2019). While near the oxygen minimum layer, a low P50 combined with relatively thin lamellar diffusion distances and a gill surface area (relative to body mass) that is larger than that of any other studied elasmobranch (Wootton et al., 2015) should facilitate better O2 diffusion into the blood from the relatively cold hypoxic water. The P50 values of the shortfin mako shark (15 and 25°C) and the blue shark (10°C) are also relatively low for elasmobranchs, only about 1–3 mmHg greater than those of the bigeye thresher shark, but neither shortfin mako sharks nor blue sharks routinely spend prolonged periods in hypoxic waters (Bernal et al., 2009). However, a low P50 likely only confers a significant physiological advantage in low environmental oxygen when it occurs in combination with other traits, such as the relatively large gill surface and thin lamellae of the bigeye thresher shark.
The ecophysiological significance of reduced and reverse temperature-dependent Hb–O2 affinity
Temperature-independent Hb–O2 binding affinity was first reported in Hb from the Atlantic bluefin tuna (Thunnus thynnus), and it was proposed that this trait may prevent thermally induced changes to Hb–O2 affinity, and thus abate disturbances to O2 uptake, in the face of large and sometimes rapid changes in environmental temperature associated with their large latitudinal and vertical (depth) movements (Rossi-Fanelli and Antonini, 1960). It seems reasonable that temperature-independent Hb–O2 affinity enables O2 uptake over a broad range of ambient temperatures, as several fishes that are tolerant of a wide range of temperatures (i.e. eurythermal), including both ectothermic and regionally endothermic fishes that have Hbs with reduced or reverse temperature dependence (e.g. Barlow et al., 2017; Bernal et al., 2018; Cech et al., 1994; Clark et al., 2010; Hopkins and Cech, 1994; Weber et al., 1976). Although Hb with a reduced temperature sensitivity is not exclusive to regionally endothermic fishes, it does not seem to be a coincidence that this trait is present in all lineages of regionally endothermic fishes investigated to date, nor that the temperature dependence of Hb–O2 affinity is considerably reduced in regionally endothermic fishes compared with most other vertebrates, including mammals with heat exchanging retia in their limbs or appendages (Weber and Campbell, 2011).
In regionally endothermic sharks such as the shortfin mako and common thresher shark, temperature-independent Hb–O2 affinity likely prevents body temperature from affecting the in vivo blood–O2 affinity, which should maintain a relatively constant blood–O2 affinity from the coldest to the warmest tissues. This may protect Hb–O2 unloading in the colder tissues, matching O2 supply to O2 demand irrespective of tissue temperature (Clark et al., 2008). In bigeye thresher sharks, Hb with a reduced temperature dependence may be linked to their thermal ecology and diurnal dive patterns, in which they spend the day in cold water (<10°C) deep below the thermocline, but ascend into the warmer upper mixed layer at night (Aalbers et al., 2021; Nakano et al., 2003; Sepulveda et al., 2019; Weng and Block, 2004). These diel migrations subject bigeye thresher sharks to abrupt changes in water temperatures ranging between 5 and 25°C (Aalbers et al., 2021; Sepulveda et al., 2019). This requires that O2 uptake is effective at the extreme temperatures, so a reduced effect of temperature on Hb–O2 affinity may ensure that environmental temperature does not excessively shift blood P50 as bigeye thresher sharks move between warm and cold water. At high Hb–O2 saturation levels, the bigeye thresher shark OEC right shifts with increasing temperature (Fig. 3D), although Hb–O2 affinity remains relatively high compared with that of other species such as the blue shark (Fig. 3C). The absence of a substantial right shift of the OEC at high O2 saturations and high temperatures may ensure that large changes to internal temperatures do not impair Hb–O2 unloading to muscles, which are specialized to function over a broader temperature range than in sympatric species (Stoehr et al., 2020). Swordfish exhibit similar diurnal distributions to bigeye thresher sharks, and the temperature dependence of Hb–O2 affinity is comparable between the two species, as are some other aspects of their physiology (Morrison et al., 2022; Sepulveda et al., 2010; Stoehr et al., 2020).
Summary
Here, we show that whole-blood Hb–O2 affinity is temperature independent for the regionally endothermic common thresher shark. Temperature-independent Hb–O2 affinity was previously shown in blood from the shortfin mako shark and was corroborated in this study. We also show that the bigeye thresher shark P50 is insensitive to temperature, and is relatively low compared with that of most sharks and marine teleosts (Harter et al., 2022; Morrison et al., 2015). This potentially indicates a tolerance to hypoxia and may allow bigeye thresher sharks to exploit depths proximal to the upper reaches of the oxygen minimum layer. Blue shark and spiny dogfish blood–O2 affinity decreased with increasing temperature, as expected for these species. In regionally endothermic sharks such as the shortfin mako shark and the common thresher shark, temperature-independent Hb–O2 affinity may avert excessive decreases in Hb–O2 affinity in warm tissues, while also preventing Hb–O2 affinity from being too high to unload sufficient O2 to the cold tissues.
Haemoglobins with a low temperature dependence of O2 binding have been reported in all studied groups of regionally endothermic fishes, although ΔH′ varies among species and not all species exhibit reductions in ΔH′ (e.g. bigeye tuna, Thunnus obesus; Lowe et al., 2000). The oxygenation-dependent release of allosteric effectors such as ATP and Bohr protons contributes endothermically to ΔH′, causing reductions or reversals in the temperature sensitivity of Hb from regionally endothermic as well ectothermic fishes (Ikeda-Saito et al., 1983; Larsen et al., 2003; Morrison et al., 2022; Nelson et al., 2019; Weber et al., 2010). ATP has been implicated as an important effector of Hb from the regionally endothermic porbeagle shark (Larsen et al., 2003), although further functional, structural and molecular studies of Hbs from regionally endothermic sharks, as well as closely related ectothermic sharks, should provide insight into the evolution of this trait and its physiological and ecological significance.
Acknowledgements
The authors thank Anne Kim (Department of Zoology, UBC) for help with the OEC experiments, as well as Scott Aalbers and Jennifer Thirkell (Pfleger Institute of Environmental Research) for field and logistical assistance. We also thank Dr Richard Brill and Dr Peter Bushnell for their assistance developing parts of this work.
Footnotes
Author contributions
Conceptualization: P.R.M., D.B., C.A.S.; Methodology: P.R.M., D.B., C.A.S., C.J.B.; Formal analysis: P.R.M.; Resources: D.B., C.A.S., C.J.B.; Writing - original draft: P.R.M.; Writing - review & editing: D.B., C.A.S., C.J.B.; Supervision: C.J.B.; Funding acquisition: D.B., C.A.S., C.J.B.
Funding
This work was supported by the National Science Foundation [1354593 to D.B., 1354772 to C.A.S.]. Funding for C.A.S. was also provided by the NOAA Saltonstall Kennedy Program [NA17NMF4270216] as well as the California Ocean Protection Council, under Grant Agreement no. C0303000, Project #R/OPCSFAQ-07 through the Sea Grant College Program. Funding was also provided by Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery grants to C.J.B. (261924-13; 2018-04172), and a Natural Sciences and Engineering Research Council of Canada Postgraduate Scholarship to P.R.M.
Data availability
All relevant data can be found within the article and its supplementary information.
References
Competing interests
The authors declare no competing or financial interests.