ABSTRACT
Ants are famous in the animal kingdom for their amazing load-carrying performance. Yet, the mechanisms that allow these insects to maintain their stability when carrying heavy loads have been poorly investigated. Here, we present a study of the kinematics of unloaded and loaded locomotion in the polymorphic seed-harvesting ant Messor barbarus. In this species, large ants have larger heads relative to their size than small ants. Hence, their center of mass is shifted forward, and even more so when they are carrying a load in their mandibles. We tested the hypothesis that this could lead to large ants being less statically stable than small ants, thus explaining their lower load-carrying ability. We found that large ants were indeed less statically stable than small ants when walking unloaded, but they were nonetheless able to adjust their stepping pattern to partly compensate for this instability. When ants were walking loaded on the other hand, there was no evidence of different locomotor behaviors in individuals of different sizes. Loaded ants, whatever their size, move too slowly to maintain their balance through dynamic stability. Rather, they seem to do so by clinging to the ground with their hind legs during part of a stride. We show through a straightforward model that allometric relationships have a minor role in explaining the differences in load-carrying ability between large ants and small ants, and that a simple scale effect is sufficient to explain these differences.
INTRODUCTION
The locomotion of animals can be described as a succession of strides that follows a specific inter-leg coordination pattern called gait (Alexander, 2003). In hexapod animals such as insects the most common gait is the alternating tripod (Delcomyn, 1981), in which the animal walks by alternating the movement of two distinct sets of legs (the ipsilateral front and hind leg and the contralateral mid leg, e.g. L1, L3, R2 and R1, R3, L2, respectively, with L for left and R for right), each of which forms a tripod supporting the body. In its ideal form, the two tripods perfectly alternate: all the legs in one tripod group simultaneously lift-off while all the legs of the other tripod group are still on the ground. However, depending on various features of their locomotion, insects can also adopt more complex gait. For example, the pattern of leg coordination can change with locomotory speed (Bender et al., 2011; Wosnitza et al., 2013; Mendes et al., 2013; Wahl et al., 2015), walking curvature (Zollikofer, 1994a) and forwards or backwards movement (Pfeffer et al., 2016). Insects also adapt their gait according to the features of the terrain on which they are moving, for example when they walk on a non-level substrate (Seidl and Wehner, 2008; Reinhardt et al., 2009; Grabowska et al., 2012; Ramdya et al., 2017; Wöhrl et al., 2017) or when they climb over obstacles (Watson et al., 2002). Another factor that is known to have an effect on leg coordination during locomotion in terrestrial vertebrates (Jagnandan and Higham, 2018) but that has been less well studied in insects is the change in the total mass an individual has to put in motion. Changes in total mass can be progressive or sudden and can occur in a variety of situations: in female insects during egg development and after oviposition, during autotomy (the voluntary shedding of a body segment; Fleming and Bateman, 2007; Lagos, 2017) or, in the most common case, when insects are transporting food, either internally, after ingesting liquid, or externally, in their mandibles. In all these situations, the change in total mass induces a shift in the center of mass of the insect, which can profoundly affect its locomotion.
Ants offer a very good model to study the effect of changes in total mass on walking kinematics for four reasons. First, they are notorious for their load-carrying ability and can routinely transport loads (prey items, seeds, nest material, nestmates and brood) weighing more than ten times their own mass over tens of meters (Bernadou et al., 2016). When an item is too heavy be carried alone, ants can also walk backward to their nest and drag it (Bernadou et al., 2016), or they can perform collective load transport (Czaczkes and Ratnieks, 2013). Second, the food collected by ants can be transported internally or externally. The shift in their center of mass can thus vary both in intensity and direction, which is likely to disrupt their walking kinematics in different ways and makes ants an interesting biological model for the study of the biomechanics of load transport. Third, as a result of the high number of species in their taxon (Hölldobler and Wilson, 1990), the size and shape of ant bodies is extremely variable, which probably affects the kinematics of their locomotion differently. And fourth, ants live in very diverse environments and can be subterranean, ground-living or arboreal (Hölldobler and Wilson, 1990), which is bound to constrain their movements and affect their locomotion (Gravish et al., 2013; Seidl and Wehner, 2008; Reinhardt et al., 2009; Wöhrl et al., 2017).
The effects of changes in total mass due to load carriage on the walking kinematics of ants have been poorly explored in the ant literature. The main effect of carrying a load in the mandibles is to induce a forward shift of the center of mass of the system formed by the ant and the load they carry. However, according to Hughes (1952), insects could counter this effect and achieve balanced locomotion by using static stability, i.e. by keeping the projection of their center of mass on the horizontal plane within the polygon formed by the legs simultaneously in contact with the ground (called the polygon of support). In fact, this is what loaded ants do. For example, Cataglyphis fortis workers ensure static stability by placing their front legs in a more forward position when loaded than when unloaded and by reducing their stride length (Zollikofer, 1994b). In Atta vollenweideri, whose foraging workers carry elongated pieces of grass over their head, ants increase their mechanical stability by increasing the number of legs simultaneously in contact with the ground. They do so by increasing over consecutive steps the overlap between the stance (retraction) phase of the supporting tripod and some of the legs of the other tripod (mostly the front leg) and by dragging their hind legs during the swing (protraction) phase (Moll et al., 2013). These ants also adjust the angle of the load they carry so that the projection of their center of mass on the horizontal plane remains within the polygon of support (Moll et al., 2013).
In this paper, we studied the effect of load carriage on the walking kinematics of the seed-harvesting ant Messor barbarus, an ant species that is characterized by a highly polymorphic worker caste, i.e. a high variability in the size of individuals within the same colony. In addition, this polymorphism is characterized by allometric relationships between the different parts of the body (Bernadou et al., 2016), which means that large workers are not an enlarged copy of small workers but that some of their body parts are disproportionately larger or smaller than those of small workers (Bonner, 2006). In fact, relative to their mass, their legs are shorter and their head larger than those of small workers. Here, we hypothesized that this allometry could lead to differences in unloaded and/or loaded locomotion in different-sized workers and thus could explain the differences observed in their load-carrying ability (Bernadou et al., 2016). To test this, we ran an experiment in which we compared the kinematics of workers tested first unloaded and then carrying loads whose relative mass we varied in a systematic way across different-sized ants.
MATERIALS AND METHODS
Study species and rearing conditions
We used workers from a large colony (∼5000 individuals) of Messor barbarus (Linnaeus 1767) collected in April 2018 at St Hippolyte (Pyrénées Orientales) on the French Mediterranean coast. Messor barbarus is a seed harvester ant whose mature colonies can shelter several tens of thousands of individuals (Cerdan, 1989). Its workers display a polymorphism characterized by a continuous monophasic allometry between head mass and thorax length (Heredia and Detrain, 2000; Bernadou et al., 2016). For the sake of clarity, we will refer in this study to the (mesosoma+petiole) part of the ants as the ‘thorax’, even if in ants it contains some segments of the abdomen. Individuals in the colony ranged from 1.5 to 40 mg in mass (mean±s.d. for a random sample of 430 workers: 6.9±5.1 mg) and from 2 to 15 mm in length. The whole colony was kept in a box (L×W×H: 50×30×15 cm) with Fluon®-coated walls to prevent ants from escaping. Ants nested inside test tubes (length: 20 cm; diameter: 2.5 cm) covered with opaque paper. They had access to water ad libitum and to seeds of various species (canary grass, niger, oats). The experimental room was kept at a constant temperature of 28°C and under a 12 h:12 h light:dark regime.
Experimental setup
The setup we used in our experiment was designed and built by a private company (R&D Vision, France. http://www.rd-vision.com). It consisted of a walkway (160×25 mm) covered with a piece of black paper (Canson® Mi-Teintes®, fine grain side, 160 g m−2) on which the ants walked during the experiment. The walkway was surrounded by five synchronized high-speed video cameras (JAI GO-5000M-PMCL: frequency: 250 Hz; resolution: 30 µm pixel−1 for the top camera, 20 µm pixel−1 for the others), one placed above the walkway and four placed on its sides (Fig. 1). Four infrared strobe spots (λ=850 nm; pulse frequency: 250 Hz), synchronized with the cameras, were used to illuminate the walkway from above, allowing a better contrast between the ants and the background on the videos. The temperature on the walkway was monitored with an infrared thermometer (MS pro, Optris, USA, http://www.optris.com). Over the course of the experiment the temperature was 28±1.4°C (mean±s.d.).
Experimental protocol
All experiments were performed between April and July 2018. On the first day of a week in which we tested ants, we installed a bridge between the colony and a box in which a few seeds were placed. We then collected one ant out of three in 1 h that carried a seed back to the colony. These ants were kept apart and used for the experiment over the following days. We also collected a few ants (weighing between 10 and 15 mg) to dissect their Dufour gland in order to create an artificial pheromone trail in the middle of the walkway (Heredia and Detrain, 2000). Since ants tended to follow the trail, this increased the chance of obtaining a straighter path in the middle of the walkway, which allowed us to neglect the effect of path curvature on ant kinematics (Zollikofer, 1994a). In order to extract the Dufour gland, ants were first anesthetized by placing them in a vial plunged in crushed ice, then killed by removing their head and fixed on their back with insect pins under a binocular microscope. Their gaster was opened transversally with a scalpel following the first sternite and the ventral part was pulled away. The poison gland and the fat gland were then gently removed until the Dufour gland became visible. This latter was then collected and placed in a hexane solution to extract the trail pheromone. We used a concentration of 1 gland per 20 µl, which has been shown to be sufficient to elicit a clear trail-following response in M. barbarus workers (Heredia and Detrain, 2000).
Each time an ant was tested, it was picked from the group of ants that had been separated on the first day of the week, then weighed and isolated in a small box with access to water. The ant was first tested unloaded and then loaded with lead fishing weights whose mass ranged from 2 to 100 mg. The ant and its load were chosen in order to cover the range of ant mass found in our colony and a range of load ratio [defined as: 1+(load mass/ant mass), see Bartholomew et al., 1988] between 1.2 and 7, corresponding to that of most of the items spontaneously seized and carried by M. barbarus workers in the field (Bernadou et al., 2016).
After every fifth ant tested, we made an artificial trail on the walkway by depositing a 1 µl droplet of the Dufour gland extract with a 10 µl syringe at 1 cm intervals. To reduce stress, ants were transferred from their individual boxes to the walkway by letting them climb up and down on a pencil. Once on the walkway, the movement of the ant was recorded as soon as it started to walk along a more or less straight path. The ant was then captured at the end of the walkway and anesthetized by placing it in a vial plunged in crushed ice. It was then fixed dorsally with Plasticine under a binocular microscope with its head maintained horizontally. This allowed us to put a drop of superglue (Loctite, http://www.loctite.fr) on the top of its mandibles and to attach a fishing weight. The same procedure as for unloaded ants was then used to film loaded ants. At the end of the experiment, the ant was killed and we weighed its head, thorax (with the legs) and gaster separately to the nearest 0.1 mg with a precision balance (NewClassic MS semi-micro, Mettler, Toledo, USA). Whether unloaded or loaded, we filmed all ants for at least three strides. We defined a stride period as the time elapsed between two consecutive lift-offs of the right mid leg. For our analysis, we cropped the videos to a whole number of strides.
Data extraction and analysis
When several video recordings of the same ant had been made, we selected the videos (top view and one of the four side views) in which the ant had the straighter path. As a criteria for path straightness, we calculated the ratio of the distance actually traveled by ants (based on the horizontal trajectory of their center of mass) on the straight line distance between the first and last point of their trajectory and considered that the path was sufficiently straight when this ratio was lower than 1.2.
Several points were tracked with the software Kinovea (v. 0.8.15, https://www.kinovea.org) on the selected videos. On the top view, the (X,Y) coordinates of the following points were determined on each video frame (Fig. 2A–C): the extremity of the mandibles, the neck, the junction between the petiole and the gaster, the extremity of the gaster and the position of the leg tarsus during the stance phases. In addition, we tracked the extremity of the load carried by loaded ants. The side view (Fig. 2B–D) was used to determine the state of each leg during locomotion (i.e. in stance phase, swung or dragged). Assuming a homogeneous distribution of the mass within each body parts, we then computed for each frame the approximate (x,y) position of the CoM of the three main body parts (plus the load) as the mean of the (x,y) coordinates of the points located at their two extremities. Finally, we computed the horizontal position of the center of mass (CoM) of the ants on each video frame as the barycenter of the CoM of their head, thorax and gaster weighed by their respective mass. In addition, we used these tracked points in order to compute the length of the main body parts of the ants on each frame. The length of their main body parts was then computed as the average length over all frames of a video.
For each video frame, we visually determined the state of each leg during locomotion (i.e. in stance phase, swung or dragged) on the side view of the ant and recorded the spatial position of the leg tarsus during the stance phases on the top view. These positions were expressed in a Cartesian coordinate system centered on the petiole of the ant, with the x-axis corresponding to the longitudinal axis of its body and the y-axis to the transverse axis (Fig. 2). In order to compare ants of different sizes, all distances were normalized to the body length of the ant (calculated from the tip of the gaster to the tip of the mandibles).
We computed the duty factor for each leg as the fraction of the stride that the leg was in contact with the ground (Ting et al., 1994; Spence et al., 2010). For each leg, we also computed the mean position of the tarsus end at lift-off (posterior extreme position, PEP) and at touch-down (anterior extreme position, AEP) by averaging the positions of the tarsus end over the strides we filmed. Since the path followed by ants was straight, we averaged the values of the right and left leg of each pair of legs when computing the duty factors and leg positions. We expressed these mean positions at lift-off and touch-down in a polar coordinate system. As we did not track the positions of the leg coxae, we computed three virtual points at equidistant intervals between the neck and petiole and used them as origins for the polar coordinate system associated with the three pairs of legs. For all legs, we then computed the polar coordinates (r,φ) of the position of the tarsus end at lift-off and touch-down in their respective polar coordinate system (Fig. 2C). Following Wosnitza et al. (2013) and Wahl et al. (2015) we calculated step amplitude rather than stride length. For each leg, we computed the step amplitude (in mm) by averaging the distances between PEP and AEP positions in the ant Cartesian coordinate system. Again, because the path followed by the ant was straight, we averaged the values of the right and left leg of each pair of legs when computing step amplitude.
We studied inter-leg coordination by comparing the time of lift-off of every leg to the time of lift-off of the right mid leg (Wosnitza et al., 2013; Wahl et al., 2015). More precisely, we computed, for each leg, the time lag between the leg lift-off and the last lift-off of the right mid leg. We then divided the value of the time lag by the time elapsed between two successive lift-offs of the right mid leg. This value was expressed as a phase shift between –π and π for each leg. Finally, we used circular statistics (Batschelet, 1981) to compute the mean vector of the distribution of the phase shifts for specific groups of ants. As an indication of how ant gait was close to an ideal alternating tripod locomotion, we also computed the tripod coordination strength (TCS) (Wosnitza et al., 2013; Wahl et al., 2015; Ramdya et al., 2017). This index can take values between 0 and 1. A TCS of 1 corresponds to a perfect alternating tripod gait while a TCS of 0 means that the ant performed a completely different type of gait.
Following Moll et al. (2013), we also computed for each ant the change over time of the static stability margin (SSM) during locomotion. For every video frame, the SSM was defined as the minimum distance between the projection of the ant CoM on the horizontal plane and the edges of the polygon formed by all legs in contact with the ground, including the dragged legs. The SSM is positive if the projection of the CoM lies inside the polygon, negative otherwise. We used the expression ‘statically stable locomotion’ to refer to the parts of the locomotion in which the ant managed to maintain static stability (i.e. when the SSM was positive) and the expression ‘statically unstable locomotion’ to refer to the parts of the locomotion in which it was not the case (i.e. when the SSM was negative). For each ant, we computed the proportion of time it was performing statically stable locomotion.
In order to investigate whether the decrease in stability we observed in large ants was the same as that one should observe mechanically because of the forward shift of the center of mass of the body or whether this decrease was less than expected because large ants adjusted their gait in order to maintain their stability, we computed a model of an ideal alternating tripod gait and compared the stability data generated by this model to those calculated in real ants. First, we used the allometric relationships we measured between the different body parts of our ants (Table S1, Fig. 3A) to model virtual ants of different sizes. Second, we assumed that all ants walk with the same stepping pattern. We computed the leg position as the mean value of the leg positions observed in our experiment for all ants, corrected for the differences in leg length between ants of different sizes (Table S1, Fig. 3B). Finally, we followed the same procedure as that described above in order to compute the proportion of statically stable locomotion for these virtual ants. The data we obtained with these virtual ants were then compared with those obtained in real ants.
For the unloaded condition, all variables y were expressed as a power law function of ant mass M: y=a×Mb. For each variable, we give the value of the coefficients a and b, as well as the value of the variables predicted by the statistical model for the mean ant mass (11.8 mg). For the loaded condition, because we tested the same ants first loaded and then unloaded, we computed for each variable the ratio of its value between the loaded (yl) and unloaded (yu) condition and expressed it as a power law function of both ant mass (M) and LR: yl/yu=c×Md×LRe. For each variable, we give the value of the coefficients c, d and e as well as the value of the ratio predicted by the statistical model for mean ant mass and a load ratio of one. A positive value for a coefficient (i.e. c, d or e) means that the value of the response variable in loaded condition increases compared with unloaded condition when the explanatory variable increases and vice versa.
We performed all data analysis and designed all graphics with R (v. 3.5.1) run under RStudio (v. 1.0.136). The dataset used for this study is available at http://doi.org/10.5281/zenodo.2646485
RESULTS
Unloaded ants: influence of body mass
Stride frequency (F1,43=59.92, P<0.001) decreased with ant mass (Table 1). However, step amplitude increased with ant mass for every leg (front leg: F1,43=204.45, P<0.001; mid leg: F1,43=193.57, P<0.001; hind leg: F1,43=122.73, P<0.001). As a result, speed did not depend on ant mass (F1,43=0.33, P=0.566). The duty factor of all legs increased with increasing ant mass, particularly for the front (F1,43=37.04, P<0.001) and mid (F1,43=23.06, P<0.001) legs (Fig. S1A–C). Therefore, compared with small ants, large ants tended to have more legs in contact with the ground during a stride (F1,43=37.28, P<0.001). The front and mid legs were almost never dragged by ants. However, independent of ant mass, hind legs were dragged during 21% of a stride on average.
In an ideal alternating tripod gait, all legs of a tripod lift-off simultaneously. In actual locomotion however, there is no such perfect synchronization. Nevertheless, the alternating tripod gait model still holds if the time interval between the lift-off of the three legs of the same tripod is small. Fig. 4A shows that the ants' gait is very close to an ideal tripod gait (see also Fig. S2A). However, for large ants, the front legs tended to lift-off slightly after the mid and hind legs of the same tripod. As a result, the TCS slightly decreased (F1,43=13.43, P<0.001).
Fig. 5A shows the Cartesian coordinates of the legs expressed in units of body length for three categories of ants based on their mass both at lift-off (PEP, on the left) and at touch-down (AEP, on the right). All legs were positioned at a greater distance from their point of insertion in the body with increasing ant mass both at touch-down (AEP: front legs: F1,43=1572.03, P<0.001; mid legs: F1,43=1212.90, P<0.001; hind legs: F1,43=670.01, P<0.001) and lift-off (PEP: front legs: F1,43=207.43, P<0.001; mid legs: F1,43=870.76, P<0.001; hind legs: F1,43=1060.18, P<0.001). The angle between the front leg and the longitudinal axis (Fig. 2) at touch-down tended to decrease with ant mass (AEP: F1,43=15.47, P<0.001) while, at lift-off, the angle between the leg and the longitudinal axis slightly increased with ant mass for all three legs (PEP: front legs: F1,43=11.48, P=0.002; mid legs: F1,43=7.77, P=0.008; hind legs: F1,43=19.86, P<0.001).
The static stability margin decreased during a stride and reached a local minimum value just before touch-down of one of the front legs (Fig. 6A,B). The percentage of time an ant moved in statically stable locomotion (i.e. with an SSM<0) decreased with ant mass (F1,43=4.96, P=0.031). This can be explained by the forward shift in the horizontal position of the CoM in ants of increasing size (F1,43=85.77, P<0.001). When the SSM was negative, it was always due to the CoM being in an anterior position to the polygon of support. Therefore, small ants have generally a more balanced locomotion than large ants.
Whether large ants are able to adjust their locomotory behavior to compensate, at least partly, for the decrease in stability as a result of their CoM being placed in a more forward position can be assessed by our ideal alternating tripod gait model. The coefficient a describing the influence of ant mass on the proportion of time our model ants perform statically stable locomotion is −0.092 [CI: −0.132; −0.051]. This value is significantly smaller than that found in real ants (−0.014 [CI: −0.026; −0.001], see Table 1). Thus, if all ants were walking with the same ideal alternating tripod gait, the effect of the forward shift of the CoM on the proportion of time large ants perform statically stable locomotion would be more important than what we observed in our experiment. We conclude that the changes observed above in the kinematics features of locomotion between small ants and large ants lead to an increase in static stability in large ants.
Loaded ants: influence of ant body mass and load ratio
Fig. 7 and Table 2 show the values of load ratio (LR) tested for ants of different body masses. Depending on the value of the LR, ants exhibited two kinds of behaviors when loaded. They could either keep the load lifted above the ground during locomotion or they could maintain their head in a very slanted position and push the load in front of them (see Movies 1–3 for illustrations). We called the first behavior ‘carrying’ and the second ‘pushing’. The criteria we used to distinguish between the two behaviors is based on whether or not the load glued on the ant mandibles was in contact with the ground during locomotion. We used a logistic regression to investigate the effect of ant mass, LR and the interaction between these two variables, on the probability of using pushing versus carrying behavior to transport the load. The results show a marginally significant interaction between ant mass and LR (z=−1.985, P=0.047). Pushing generally occurred for LR>5 for ants weighing >10 mg, while for ants of lower body mass, both carrying and pushing could be observed for LR>4. We will only consider ants that carry their load in the following analyses.
Independent of ant mass, stride frequency decreased with increasing LR (F2,42=21.58, P<0.001; Fig. S3A). Step amplitude was independent of LR for front and mid legs but tended to decrease for hind legs (F2,42=2.96, P=0.062). Consequently, ant speed decreased with increasing LR (F2,42=21.58, P<0.001; Fig. S3B). However, for ants transporting equivalent loads there was no effect of body mass on both stride frequency and step amplitude.
Independent of ant mass, the duty factor increased for the front (F2,42=58.32, P<0.001), mid (F2,42=25.41, P<0.001) and hind legs (F2,42=11.71, P<0.001) for increasing load ratios (Fig. S1D–F). The mean number of legs simultaneously in contact with the ground increased for increasing load ratios independent of ant size and, to a lesser extent, increased for increasing body mass independent of load ratio (F2,42=56.94, P<0.001). Similarly to when ants were unloaded, the front and mid legs were almost never dragged during locomotion when ants were loaded. The proportion of time the hind legs were dragged decreased significantly as soon as the ant was loaded, with no influence of either ant mass or load ratio.
Fig. 5B shows the changes in leg positions in unit of body length at lift-off (PEP, on the left) and touch-down (AEP, on the right) for ants carrying either small (LR<3.5) or large (LR>3.5) relative loads. Independent of ant mass, the angle between the front legs and the longitudinal axis increased with increasing load ratio at lift-off (PEP: F2,42=3.53, P=0.038). Ants also placed their mid legs further away from the body at touch-down (AEP: F2,42=6.03, P=0.005) and the angle between the mid legs and the longitudinal axis slightly increased with LR at lift-off (PEP: F2,42=3.45, P=0.041). Independent of ant mass, the hind legs were placed further away from the body with increasing LR at touch-down (AEP: F2,42=3.45, P=0.041) and the angle with the body longitudinal axis increased with increasing ant mass independent of LR (AEP: F2,42=4.73, P=0.014). Finally, at lift-off the angle of the hind legs increased with LR independent of ant mass and increased with ant mass independent of LR (PEP: F2,42=5.65, P=0.007).
While performing loaded locomotion, ants did not exhibit the same inter-leg coordination pattern than during unloaded locomotion (Fig. 4). First, there was more dispersion in phase shift between legs for loaded ants, especially for the hind legs and for high LR values (>3.5, see Fig. 4B2). Second, the three legs of the same tripod tended to lift-off in a specific order (i.e. mid leg→front leg→hind leg). This was especially clear for the hind leg, which was the last to lift-off in each tripod. This order seems to be more strictly followed for higher LR values and for large ants (Fig. 4B2). As a result, the value of TCS decreased for increasing LR (F2,42=12.48, P<0.001).
Independent of ant size, the percentage of time ants were performing statically stable locomotion decreased with increasing LR in loaded ants (F2,42=34.05, P<0.001). When the minimum static stability margin became negative, it was always because the CoM was anterior to the polygon of support.
DISCUSSION
In this study, we investigated the kinematics of locomotion of unloaded and loaded ants of the polymorphic species M. barbarus. We found that, relative to their size, small ants were able to carry larger loads than large ants. Small ants also were more stable than large ants; all ants, whatever their size, reduced their speed when carrying loads of increasing mass. The locomotion of unloaded ants was very close to an ideal alternating tripod gait. This allowed them to perform a rather statically stable locomotion. On the other hand, loaded ants were mostly statically unstable and their gait changed to more tetrapod-like locomotion, wave gait locomotion and hexapodal stance phases (see Fig. S2B for an illustration).
Unloaded ants
In M. barbarus, large ants have, relative to their size, larger heads than small ants (Heredia and Detrain, 2000; Bernadou et al., 2016). This means that their center of mass is located in a more anterior position compared with small ants. Large ants are thus more likely to be off balance than small ants. Nevertheless, our ideal alternating tripod gait model shows that, somehow, they are able to compensate for the instability caused by the forward shift of their center of mass. Our results suggest that this could be done through a change in leg positioning and gait parameters. For example, large ants lifted off their front legs at a position further away from their body than small ants. Note however, that the increase of the front leg distance from the body with ant mass (i.e. 0.428 [0.368; 0.487], see Table 1) is significantly greater than what would be expected by the allometric relationship between front leg length and ant mass (i.e. 0.310 [0.295; 0.325], see Table S1). In other words, large ants lifted off their front legs relatively further away from the coxae than small ants. In addition, at lift-off, the angle between the front legs and the longitudinal axis increased with ant mass. Therefore, the front legs lifted off in a more anterior position in large ants than in small ants. The position of the front legs at lift-off is of crucial importance for static stability since the minimum values of SSM arise just before the contralateral front leg touches down (Fig. 6). Consequently, lifting off the front leg in a more anterior position allows large ants to decrease the proportion of unstable static locomotion. This leads to a decrease in relative step amplitude for front legs with increasing ant mass (again by comparing the respective coefficients in Table 1 for the step amplitude of front legs and in Table S1 for the relationship between front leg length and ant mass). In addition, the duty factor increased for all legs, which means that large ants kept their legs in contact with the ground for a proportionally longer time during a stride than small ants. As a result, the mean number of legs in contact with the ground increased with increasing ant mass, which contributes to increase static stability in large ants. In conclusion, the differences in morphology between ants of different sizes do induce a less statically stable locomotion in large ants but this effect is reduced by the fact that they are able to adjust their stepping pattern to compensate, at least partly, for this instability.
Carrying capacity
Carrying capacity, defined here as the value of the load ratio for which the transition (i.e. the 50% probability) between carrying and pushing occurred, was greater for small ants than for large ants (Fig. 7). Therefore, small ants were able to carry relatively heavier loads than large ants. This is concordant with the results obtained by Bernadou et al. (2016) in the same species. However, in their study, the carrying capacity was related to the transition occurring between carrying and dragging, not between carrying and pushing as in our study. To our knowledge, pushing behavior has never been described in field observations of foraging seed-harvesting ants. Pushing probably occurs very rarely in the field because of the friction forces generated by the heterogeneities of the natural substrate on which the ants are moving. These friction forces would rapidly lead ants to abandon altogether their load or turn around and start dragging it while moving backward to their nest. Pushing is probably an artefact caused by both the gluing of the load on the ants' mandibles and the smoothness of the substrate on which the ants are moving.
The differences in load-carrying capacity can be accounted for by two non-exclusive explanations. The first is related to the well-known scale effect, while the second is related to differences in the locomotion and/or the morphology (induced by allometric relationships) of ants of different sizes.
which is the equation of the black line in Fig. 8. As can be seen in Fig. 8, the predictions of the carrying capacity for our model ants of different sizes with same morphology is close to the curve representing the 50% probability of carrying a load versus pushing it that we obtained from our experiment. It is also close to the curve representing the 50% probability of carrying a load versus dragging it from the field experiments by Bernadou et al. (2016) in which the ants transported food items of various sizes deposited on their foraging trails. Therefore, it seems that ants start pushing in our experiment for about the same load ratio values as they start dragging in Bernadou et al. (2016) and that this can be explained mainly by the scale effect.
Nonetheless, one cannot exclude that the differences observed in the locomotor behavior between small and large ants could also partly explain the differences in carrying capacity. Table 2 indeed points out some differences in the kinematics of ants of different sizes, independent of load ratio. However, despite slight changes in the mean number of legs in contact with the ground, TCS values and hind leg positioning with increasing ant mass, we could not find any particular logic that could help to explain the differences in carrying capacity between small and large ants. Therefore, it seems to us that the observed differences in carrying capacities in ants of different sizes can be well explained by the scale effect. Note that a recent study in Atta cephalotes found exactly the opposite result: that the carrying capacity is constant and independent of ant mass (Segre and Taylor, 2019). Further studies are needed in order to determine the origin of these differences.
Influence of load ratio on locomotion
The main effect of carrying a load for an ant is to shift its CoM forward. As a consequence, the CoM is located closer to the front edge of the polygon of support, or even lies out of it, and the SSM decreases or becomes negative, leading the ant to perform less statically stable or statically unstable locomotion. Moll et al. (2013) showed that loaded A. vollenweideri ants can reduce this effect by changing the way they carry their load: by carrying the pieces of grass blade they hold in their mandibles in a more upward, backward-tilted position they can shift their CoM in a somewhat backward position. This is of course impossible in our experiment because ants cannot adjust the position of the load glued on their mandibles. This would not happen either in the field because most seeds collected by M. barbarus are not elongated enough to be carried in the same way as pieces of grass blades in grass-cutting ants. The CoM of loaded M. barbarus workers is thus shifted forward and the percentage of time their locomotion is statically unstable during a stride increases for increasing LR (Table 2), reaching up to 90% for the highest LRs.
Such statically unstable locomotion has already been reported for insects in the literature. For instance, the cockroach Blaberus discoidalis, when moving at very high speed, often performs statically unstable locomotion and thus maintains its balance through dynamic stability (Ting et al., 1994; Koditschek et al., 2004). Dynamic stability refers to individuals keeping their balance when statically unstable by only briefly ‘falling’ forward before new supporting legs contact the ground (Moll et al., 2013). Statically unstable locomotion has also been observed by Moll et al. (2013) in loaded workers of the grass cutting ant A. vollenweideri. These authors have suggested that loaded ants could use dynamic stability in order to avoid falling over during the statically unstable part of their locomotion (Moll et al., 2010, 2013).
However, loaded M. barbarus workers move too slowly (Table 2) to maintain their balance through dynamic stability: they would fall forward before the front leg catches up. Rather, we assume that they maintain their balance by clinging to the ground with the tarsal claws located at the end of their mid and hind legs. Indeed, scanning electron microscope images of the substrate texture show that the claws can easily cling to the paper fibers (Fig. S4B). Consequently, they tend to keep more legs in contact with the ground for increasing LRs. This leads to a decrease in their stride frequency and TCS and to an increase of the duty factor of all legs (Table 2). Hind legs are of particular importance in keeping the ant balanced because they have a higher lever-arm effect. In our experiment, the percentage of time the hind legs were dragged decreased as soon as the ant was loaded independent of ant mass and load ratio (mean±CI0.95: −12.1±8.2%), probably because ants use the claws of the pretarsus in order to cling to the ground. In this respect, it would be interesting to investigate how ants maintain their balance when their adhesive prestarsal structures are blocked or when they walk on a slipping substrate (see Ramdya et al., 2017 for an example in Drosophila melanogaster). The tendency for the hind legs to lift-off after the front legs touched down also increased for increasing load ratio (Fig. 4B,C). This is coherent with the balance strategy used by ants, as the SSM is maximal at front leg touch-down (Fig. 6C,D) and thus it is less risky to lift-off the hind leg at this time. Finally, as a result of the stride frequency decrease (and because step amplitude remains constant), the speed decreased with increasing LR, which is concordant with most studies in other load-carrying ants, such as Atta colombica (Lighton et al., 1987), A. vollenweideri (Röschard and Roces, 2002), Atta cephalotes (Burd, 2000) and Veromessor pergandei (Rissing, 1982).
Reinhardt and Blickhan (2014a) showed that, during steady state locomotion, Formica polyctena uses mainly its hind legs in order to generate propulsion forces while Wöhrl et al. (2017) showed in Cataglyphis fortis that it is the mid legs that are mainly used for propulsion. In both cases however, the front legs have a brake effect on locomotion. To our knowledge, there are no studies so far that measured the ground reaction forces (GRFs) in loaded ants. Nonetheless, it is possible to infer the propulsion behavior of the legs in our experiment based on the position of their tarsi. Indeed, as shown by Endlein and Federle (2015), depending on the GRFs, the tarsi attach differently to the substrate. The morphology of the tarsal attachment of M. barbarus (Fig. S4A) is comparable to that of other ants (Federle et al., 2001; Endlein and Federle, 2008). It seems thus fair to assume that they cling to the substrate in a similar way. Like Endlein and Federle (2015), we observed in our videos two positions for the hind leg tarsi during the stance phase: on ‘heels’, during the first part of the stance phase, and on ‘toes’, during its second part (Fig. S5). This would suggest that hind legs have a ‘compression and pushing’ action in the first part of the stance phase, i.e. participate to propulsion, and then have a ‘tension and pulling’ action on the last part of the stance phase, acting as a holding point for the ants not to fall over. For mid legs, the tarsi were usually in the ‘heel’ position and were thus likely to participate in propulsion. These observations are purely qualitative as the resolution of our videos makes a quantitative analysis of these data tricky. The use of a miniature force plate (Bartsch et al., 2007; Reinhardt and Blickhan, 2014b) to compare the GRFs of unloaded and loaded ants would provide crucial insights on how the different legs of the ants contribute to the stability and propulsion of loaded locomotion.
Conclusion
We have shown in this study that unloaded M. barbarus workers display different gaits depending on their body mass. For large ants, these differences seem to be mainly explained by a compensation for the imbalance caused by their disproportionally larger head. Small ants are able to carry proportionally heavier loads than large ants and scale effect provides a simple and satisfactory explanation for this difference. Moreover, our results show that loaded ants are often statically unstable during locomotion and that they maintain their balance by clinging to the ground. Further studies are required to determine the contribution of each leg to both stability and propulsion.
Large ants are more costly to produce than small ants. So why do colonies produce them if they are less efficient in transporting loads? One answer to this question is that, although large ants have lower load carriage performance than small ants, they are nonetheless able to carry on average loads of higher masses than small ants and to seize and transport items of larger diameters with their large and powerful mandibles (fig. 3 in Bernadou et al., 2016). This could allow colonies to increase the size range of the food items retrieved to the nest so that they can enlarge their diet breadth and better match the size distribution of the food resources available in their environment (Davidson, 1978). Large ants may also play roles other than foraging in seed-harvesting ant colonies, such as removing the obstacles encountered on foraging trails, constructing and defending the nest (Rissing, 1982), cutting thick plant stalks or milling the seeds inside the nest to prepare them for consumption. The significance of our results for the foraging ecology and division of labor in M. barbarus remains therefore to be investigated.
Acknowledgements
The authors wish to thank Ewen Powie and Loreen Rupprecht who helped with data extraction, as well as Melanie Debelgarric who designed the Dufour gland extraction protocol.
Footnotes
Author contributions
Conceptualization: H.M., P.M., V.F.; Methodology: H.M., P.M., V.F.; Software: H.M.; Validation: H.M., V.F.; Formal analysis: H.M., V.F.; Investigation: H.M., G.L.; Resources: H.M., G.L., P.M., V.F.; Data curation: H.M.; Writing - original draft: H.M., V.F.; Writing - review & editing: H.M., P.M., V.F.; Visualization: H.M., V.F.; Supervision: P.M., V.F.; Project administration: P.M., V.F.; Funding acquisition: P.M., V.F.
Funding
H.M. was funded by a doctoral grant from the French Ministry of Higher Education, Research and Innovation through the SEVAB graduate school of the University of Toulouse. The Image acquisition equipment was financed by the project Serious GaRS (ref N°16004115/MP0007086) funded by FEDER-FSE Midi-Pyrénées et Garonne 2014-2020.
References
Competing interests
The authors declare no competing or financial interests.