Animal-borne video recordings from blue whales in the open ocean show that remoras preferentially adhere to specific regions on the surface of the whale. Using empirical and computational fluid dynamics analyses, we show that remora attachment was specific to regions of separating flow and wakes caused by surface features on the whale. Adhesion at these locations offers remoras drag reduction of up to 71–84% compared with the freestream. Remoras were observed to move freely along the surface of the whale using skimming and sliding behaviors. Skimming provided drag reduction as high as 50–72% at some locations for some remora sizes, but little to none was available in regions where few to no remoras were observed. Experimental work suggests that the Venturi effect may help remoras stay near the whale while skimming. Understanding the flow environment around a swimming blue whale will inform the placement of biosensor tags to increase attachment time for extended ecological monitoring.
There are considerable challenges to be overcome to find food and mates in the open ocean, particularly for smaller fishes that are non-migratory. In what may have evolved as a novel functional approach to mediate this (Gamel et al., 2019), remora fishes (family Echeneidae) adhere to the surface of other swimming organisms, including sharks, mantas, marlin, turtles, dolphins and whales, using a modified dorsal fin (Andrade, 2007; Brunnschweiler and Sazima, 2006; Fertl and Landry, 1999; Gudger, 1919; Kenaley et al., 2019; O'Toole, 2002). Their relationship is considered symbiotic, as many species of remoras feed on parasites on the host surface which would otherwise reduce host health (Cressey and Lachner, 1970; Strasburg, 1962), in return for bits of host prey, reduced locomotor cost and predation risk, and increased likelihood of meeting conspecifics (Muir et al., 1967; Silva-Jr and Sazima, 2003; Steffensen and Lomholt, 1983).
However, exactly why remoras select specific locations on a host's body is unknown. It has long been speculated that for any particular host organism, remoras may preferentially adhere to particular locations on the host body to lower host irritation, reduce drag or increase likelihood of food availability (Douglas and Calambokidis, 2001; Weihs et al., 2007). Unfortunately, remora location data are typically derived from single photographs, often at the sea surface for cetaceans, or from short observations of a host organism (Fig. 1). No studies have continuously followed the precise positions and movement patterns of remoras on a host for any period of time.
A unique opportunity to study remora position and behavior in situ became available through the development of biologging tags with video capabilities that are deployed on host species, including large baleen whales like blue whales (Balaenoptera musculus). Here, remoras were observed interacting with each other and moving along the whale in specific locations on the whale body. Their preference for specific attachment locations suggests that some physical and/or biotic conditions at those sites encourage remora adhesion. Hydrodynamic conditions, especially drag, would vary for remoras along the body of a large swimming organism, such as the blue whale.
Remora bodies are streamlined and are estimated to contribute a low ‘parasitic drag’ on hosts such as dolphins or sharks (Beckert et al., 2016a). However, drag experienced by the remora may be relatively high. Median blue whale swimming speed has been recorded at 2.1±0.5 m s−1 (7.6±1.8 km h−1) and at 3.9±0.8 m s−1 (14±2.9 km h−1) during rapid feeding bursts (Cade et al., 2016; Gough et al., 2019). Given that remoras can grow to 1 m (Lieske and Myers, 1994), riding a blue whale in those circumstances represents speeds of more than 2–4 body lengths (BL) s−1 for the remora. By contrast, most fishes are known to swim comfortably at around 1–2 BL s−1 and few can maintain speeds over 2 BL s−1 for an extended duration (Jayne and Lauder, 1996; Lauder, 2006). Although remora could potentially keep pace with a blue whale by burst swimming and resting during attachment, we hypothesized that their preferential localization on and swimming behaviors near a blue whale are, at least in part, related to the reduction of hydrodynamic drag and detachment forces, thus lowering energy use and reducing the likelihood of separation from the host. Here, we used high-performance computing and computational fluid dynamics to investigate the hydrodynamic environment around the body of a freely swimming blue whale including a case with remoras attached. We used the computational results and empirical data specific to streamlined bodies attached to surfaces (Hoerner, 1965) to determine drag on remoras and compare that information with the site specificity of remora attachment. Through this work, we hope to better our understanding of both the flow environments around an organism and the mechanisms by which specifically adapted organisms like remoras successfully attach to hosts in order to improve animal tag technologies and designs for extended periods of behavioral and ecological monitoring.
MATERIALS AND METHODS
Whales were tagged following Stanford University IACUC 30123 and NOAA Fisheries permit 16111 to J.C. Fishes used in experiments were handled humanely and ethically following New Jersey Institute of Technology/Rutgers University IACUC protocol 17058-A0-R1.
Video footage of remoras attached to blue whales [Balaenoptera musculus (Linnaeus 1758)] was opportunistically recorded from suction cup-attached, multi-sensor video bio-logging tags from Customized Animal Tracking Solutions (CATS) (Cade et al., 2018; Goldbogen et al., 2017) used for ecological tracking studies on 6–20 August 2014 off of Palos Verdes and San Diego, CA, USA. The tag was 23 cm×13 cm×4.5 cm in size, 654 g in mass, and designed to stick to the whale body surface with four suction cups at its base. All tags were deployed from a 6 m rigid-hull inflatable boat using a 6 m pole. The tag housed dual cameras, each of which recorded video at 720×1280 pixels, at a variable frame rate of 22–24 frames s−1. Accelerometers, magnetometers and gyroscopes were sampled at 40 Hz, and pressure, light, temperature and GPS at 10 Hz. Data were decimated to 10 Hz, tag orientation on the animal was corrected for, and animal orientation was calculated using custom-written MATLAB scripts (Cade et al., 2016; Johnson and Tyack, 2003). Animal speed for all deployments was determined using the amplitude of tag vibrations (Cade et al., 2018). Tag position and orientation were confirmed through observation during tracking sequences, through heading, pitch and roll calculations from tag positional sensor output, and through orientation to the whale body in videos. Using recorded data from the CATS multi-sensor camera tags, we were able to visually identify each remora's position on the whale body (Fig. 2), including its attachment relative to the blue whale's rostrum, x (m), and the velocity of the whale U0 (m s−1).
We had the opportunity to augment the CATS tag dataset using underwater video to confirm whether remoras selected different positions on the whale body in the absence of the tag and found no difference in attachment location. Underwater video was filmed as a part of scientific research under permit off the coast of southern California for the BBC television series ‘The Hunt’ produced by Hugh Pearson, in collaboration with J.C. (Fig. 1B). All procedures were conducted under approval of the National Marine Fisheries Service (permits 16111, 19116, 15271); National Marine Sanctuaries (MULTI-2017-007); and institutional IACUC committees.
To test for site specificity on the body surface of the blue whale, a Rayleigh distribution test for non-uniformity was used. If we consider the blue whale surface to be uniformly equal in terms of non-specificity of attachment environment at any location, we can test for non-random concentrations of remora positions using the Rayleigh distribution test (Brazier, 1994).
Computational fluid dynamics (CFD) analysis
Design of the numerical simulations
The finite element-based numerical solver Alya (Vázquez et al., 2016) was utilized to solve the Navier–Stokes equations of turbulent incompressible flows. Turbulence was handled by means of a large eddy simulation (LES) model whose details are given below. Equal-order finite elements (equal order interpolation of velocity and pressure) were used, and pressure was stabilized via a non-incremental fractional-step method (Charnyi et al., 2017; Lehmkuhl et al., 2019). This approach was demonstrated to conserve linear and angular momentum at the discrete level. The equations are solved forward in time to reach a steady-state by means of an energy-conserving explicit Runge–Kutta method (Capuano et al., 2017) with adaptive time stepping. The adaptive time-step procedure relies on the analytical bounding of the eigenvalues of the dynamical system to adapt the linear stability domain of the Runge–Kutta scheme for time-step maximization (Trias and Lehmkuhl, 2011).
The whale geometry was obtained from a three-dimensional scan of a commissioned scale replica at 1:100 size. The digital stereolithography file was then imported into the numerical mesh generator to build a mesh. Based on underwater observations, an average whale speed of U0=1.5 m s−1 was estimated. This free-stream velocity of water with kinematic viscosity ν=1.3×10−6 m2 s−1 and a whale length c=18 m yield a Reynolds number Rec≈22.7×106. The computational domain is a parallelepiped 9 whale lengths long in the stream-wise direction, 25 average whale diameters in the span-wise direction, and 20 average whale diameters in the vertical direction.
A set of two simulations was performed using two meshes of increasing resolution. The first one was a spin-up simulation based on a relatively coarse-grained mesh, or ‘grid’ (approximately 27 million finite elements; Fig. 3) to estimate the necessary time for a steady-state solution to be reached given the available computational resources. The steady state was reached in 8 h of wall-clock time using 720 cores of MareNostrum IV at the Barcelona Supercomputing Center. The steady-state solution was then interpolated onto the fine-resolution mesh of 42 million elements as the initial state for the high-resolution simulation whose results are discussed in this article. By doing this, 6000 core hours were saved for the 42 million simulation compared with the case of an initially uniform velocity field. At an advective Courant number of 1, the high-resolution simulation was executed for 48 h of wall-clock time using as many computing cores as for the 27 million simulation.
The spin-up solution was also used to obtain an estimation of the boundary layer thickness and hence inform the construction of a proper boundary layer grid (Fig. 3B) for the 42 million simulation. Although the two meshes contain a comparable number of elements, the 42 million simulation mesh was designed by refining the element distribution in those regions where important flow gradients were measured in the spin-up simulation. Informed by the boundary layer thickness measured in the spin-up test, the boundary layer mesh of the 42 million simulation mesh was designed to fully contain the boundary layer. It was made of 8 vertical prismatic levels with a triangular base and varying thickness from 1.26 mm in the first layer to 0.36 mm in the eighth layer. With a total of 5.5 million prisms, this boundary layer grid represents 13% of the total element count. The rest of the grid is fully unstructured and consists of tetrahedral elements of characteristic length that range from 3.6×10−4 to 2.3 m.
Large eddy simulation
Boundary conditions and wall model
Estimation of the boundary layer thickness
Pressure, friction and drag coefficients
The small value of the whale's body drag coefficient (Table S1) supports the above observations that the flow around the whale body is almost fully attached with very few, localized regions of separation and reattachment of the boundary layer. The sudden variations in friction shown in Fig. 5B indicate some of these points of separation and reattachment. However, our model did not include small-scale roughness; a smooth surface was assumed. Given the instability of the boundary layer in the simulations, roughness could easily trigger a fully turbulent boundary layer in some or most of the regions where the structure of the vorticity and velocity profiles suggest that the boundary layer remained transitional in our simulation (Figs 3 and 4; Figs S2–S4). This could lead to higher drag predicted for the whale and an attached remora, but as it is likely to increase the drag at all locations, the effect is not expected to change the conclusions about which regions have the least drag and whether those regions are preferred by remora.
The computational mesh consists of 41,926,192 finite elements and 9,001,098 grid points. To reach a steady state, the simulations were executed for 48 h using 720 cores (approximately 35 thousand core hours) on MareNostrum IV at the Barcelona Supercomputing Center. The mesh with the inclusion of the remoras has approximately the same number of grid elements and grid points. The same number of computing cores was utilized for both sets of simulations (with and without remoras). The empirical approach allowed us to calculate the drag reduction at all 32 locations on the whale after just one CFD simulation to get the boundary layer flow.
Drag reduction calculations by empirical analysis
Attached remoras happen to be shaped like an optimal streamlined surface dimple shown in Hoerner (1965), and empirical data from the field of aerodynamics shows that the independent drag coefficient, cD, of such objects is 0.04 (Hoerner, 1965). The remora body height-to-length ratio of about 10–12 is ideal in aerodynamic dimples, and the small angle of the leading end of the body is a shape known to decrease interference drag. Dimples with a steeper increase in leading-end height, as in a submarine conning tower, have much higher interference drag. The half-ellipse cross-section of the remora places most of its frontal area lowest in the boundary layer, lowering the effective dynamic pressure. The cD value of 0.04 and the boundary layer profiles from our CFD were used in Eqn 10 to calculate CDBL for remora. For use in Eqn 11, a very conservative estimate of the standard constant drag coefficient, CDFS, of 0.03 was used. This is actually the value for a rigid, remora-shaped body of revolution in the freestream – a shape like two remora connected at their dorsal surfaces. CDBL and CDFS were then used in Eqn 11 to compare the drag of the attached and freestream states and determine whether there is drag reduction in the attached state. The actual drag reductions may be of greater magnitude than reported here as work on swimming fish suggests that friction drag, and thus potentially the freestream drag coefficient (CDFS), is higher on swimming fish than a fish coasting or ‘stretched-straight’ in a flow (Anderson, 2005; Anderson et al., 2001; Lighthill, 1971). This may even be true in the case of remora heights exceeding 2 times the boundary layer thickness, in which the contribution of decreased momentum in the boundary layer to drag in the attached state decreases in comparison to that of the flow over the section of the body outside of the boundary layer. One would expect the situation to converge toward no drag reduction, but low interference drag due to the favorable remora shape and higher drag in undulatory swimming versus coasting may lead to higher drag reduction than we calculate, and even where Eqn 11 yields no drag reduction.
Experimental remora–host interaction fluid dynamics
Fluid dynamic analysis of one preserved Remora australis remora (32.6 cm total length) in a flume with a working section 170×45×45 cm (Engineering Laboratory Design, Lake City, MN, USA) was performed at the Woods Hole Oceanographic Institution Shore Laboratory (WHOI, Falmouth, MA, USA). Flow was maintained at 20 cm s−1, slower than the 1.5 m s−1 used in the simulation, but potentially similar to a small remora swimming close to the whale surface in a wake region on the whale body. A vertical light sheet was produced using a pulsed IR laser (Firefly, 300 W, 808 nm; Oxford Lasers, Shirley, MA, USA). The flow was seeded with neutrally buoyant 50 µm plastic particles and imaged using a digital video camera (SA-3, 1024×1024 pixels, 60 frames s−1; Photron, San Diego, CA, USA) with a macro photographic lens (Micro-Nikkor, 105 mm; Nikon, Melville, NY, USA) to obtain high-resolution images of a field of view of 12.5×12.5 cm. The TTL synchronized camera recorded at 60 Hz and, therefore, image pairs were acquired at 30 Hz. The camera and laser were both mounted on a robotic positioning system built at WHOI for use with the flume. This was used to move the laser and camera field of view along the remora body at 7.5 cm intervals to reconstruct flow over the entire body in high resolution. Velocity fields were calculated from the particle images using an off-the-shelf PIV analysis software package (DaVis; LaVision Inc., Ypsilanti, MI, USA). Velocity vectors were calculated from image sub-windows 32×32 pixels in size with 50% overlap, resulting in a grid of 64×64 vectors for each image pair. The velocity fields from 150 image pairs at each field of view along the remora were used to calculate an average velocity field.
Eleven tag deployments on three individual blue whales resulted in 211.5 min of video footage containing a total of 27 remoras observed at 61 locations on the whales. Remoras were identified from surface photographs and video sequences as Remora australis (Figs 1 and 2) and were estimated to be 20–40 cm BL based on proximity to the camera tag and whale morphology. Remoras were most often observed directly behind the blowhole, next to and behind the dorsal fin, and on the flank region above and behind the pectoral fin (Figs 1 and 2). In two of these regions, behind the blowhole and around the dorsal fin, remoras were observed to cluster. Notably, remoras maintained attachment to these locations during whale surfacing, which resulted in the remoras being completely out of the water for a short time. Remoras also often attached near the camera tag on the dorsal surface of the whale body (Movie 1). Two tag deployments that ended in the tag sliding around the lateral and ventral aspects of the whales did not show any remoras on the ventral surface, nor did the video sequences captured underwater by divers. Rayleigh's test for non-uniform distribution showed that the remora positions were significantly different from random (P=0.01). This indicates attachment location preference and clustering in specific locations on the whale body.
Remoras need not keep a stationary position to maintain attachment to the whale. Instead, remoras were commonly observed to lift off the whale to feed and then return to their previous position (Movie 2), or other positions on the whale. Positional changes were often accomplished by a skimming behavior (Fig. 2D; Movie 3), in which a remora maintained a distance of a couple centimeters or less above the body of the whale, swimming in a slow and controlled fashion. Skimming behavior of remoras was observed while the whale velocity was between 1.5 and 3 m s−1, and our observations suggest that remoras were not experiencing freestream conditions, which were approximately 1–3 BL s−1 for the largest known remoras recorded and as high as 15–20 BL s−1 in some of the smaller remora we consider here. Remoras also changed position using a sliding behavior (Movie 4), in which their disc maintained contact with the whale while they swam. Tail beat frequency of remoras exhibiting skimming and surfing behaviors was 0.5–2 Hz, whereas remoras swimming in the freestream above the whale used tail beat frequencies of 5–8 Hz.
CFD model of whale boundary layer
A fine-scale numerical simulation of the flow around an 18 m blue whale was used to provide flow field information for empirical calculations of drag on attached remora. We used these drag calculations to explore the possibility that remoras select their position on the whale surface based on local hydrodynamic conditions. The simulation showed that the boundary layer remains mostly attached along the body surface except for specific regions where separation of flow (S regions) and wakes (W regions), both due to surface features, was observed. In Fig. 4, fluid structure revealed by vorticity near the whale surface shows that separation is triggered by protuberances on the whale surface, specifically the blowhole, the dorsal ridges and dorsal fin. In addition, the flippers and tail flukes exhibit significant flow separation. Turbulent wakes often develop downstream from the separation regions as flow fails to reattach. Furthermore, it was observed that the boundary layer is transitional, not fully turbulent, along most of the whale body, based on the large scale and intermittent structure in the near-wall vorticity (Fig. 4) and boundary layer profile shape (Fig. 4; Figs S2–S4). Tangential velocity profiles often exhibited inflections, a common sign of instability, and almost without exception their shapes fell between that of laminar and fully turbulent profiles, also a sign of transition (Wang et al., 1996; Schlichting and Gersten, 2000). This indicates that although some body features may cause separation-triggered turbulence, the modeled surface features are not sufficient to sustain a fully turbulent boundary layer. Still, as mentioned above, we did not include small-scale surface roughness that could result in a fully turbulent boundary layer, and this could impact our calculations of drag on remora. Nevertheless, in regions of separation and wakes due to large surface structures on the whale, where more remora were observed, we expect boundary layer profiles to exhibit more instances of decreased momentum than in less obstructed regions where few to no remora are observed. The latter regions would be more likely to exhibit the typical high shear boundary layer profiles of fully turbulent boundary flow. Therefore, though our absolute drag values may be underestimates if the boundary layer flow is indeed fully turbulent, remora seeking lower drag would still be expected to attach in regions identified as having relatively lower drag by our analyses.
Drag reduction in attached remora
We determined the fraction of drag difference between the attached state and coasting in the freestream (Eqn 11) in remora at 32 locations within 10 regions on the whale surface (Fig. S4) where three types of flow were observed (Fig. 4): four regions near flow separations (S), three in the wakes of surface structures (W) and three regions of well-attached flow where few to no remora were observed to attach (NR). For ease of presentation, we ran calculations for remoras of four body heights: 0.2, 0.5, 0.9 and 1.0 times hmax, 5.3 cm, a height representing the 90th percentile for the species of remora we observed. We found that in the attached state, remora 0.2 times hmax in height experience 40–50% drag reduction in S regions, 59–84% in W regions and 6–25% in NR regions compared with coasting in the freestream. Remora 0.5 times hmax in height experience 0–38% drag reduction in S regions, 25–78% in W regions and 0–2% in NR regions. The tallest remoras were found to experience significant drag reduction in one of the S regions (S1, 3–8%), two of the W regions (W2, W3, 13–73%) and none of the NR regions. As mentioned above, any increase in the freestream drag coefficient, CDFS, over the conservative estimate of 0.03 used here yields greater drag reduction. A coefficient just 33% higher (0.04) results in drag reduction for remora of almost every size at nearly every location except at zone NR3 (Fig. S5). In general, drag reduction is highest in the W regions and lowest in the NR regions. Drag did not exceed 0.30 N for any attached remora analyzed at any location. This agrees with a straightforward estimate of ‘stretched–straight’, or rigid-body, drag on a remora hmax in height in the freestream using CDFS=0.03.
We did the same analysis for a remora skimming anteriorly on the whale at a speed of 0.5 BL s−1 and a height of 1–2 cm above the surface. The behavior increases drag, as expected, but fish 0.2 times hmax in height experience drag reduction at 9 locations skimming 1 cm off the surface (Fig. S6) and 6 locations at 2 cm off the surface (Fig. S7). In every case, only S and W regions offered drag reduction during skimming in remora; NR regions did not. Some S region locations offered drag reduction in skimming for fish heights up to 0.49–0.57 times hmax, and in W regions, 1.0 times hmax and larger. Skimming fish are swimming and, therefore, drag may be higher than we estimate as a result of body undulation, but remora swimming in the freestream would also have to undulate, and more vigorously, as was evident by the lower tail beat frequency of skimming and sliding remoras as compared with those swimming in the freestream. Also, a less pronounced or lack of drag reduction in skimming does not outweigh the benefits of attachment, but it might affect where remoras of particular sizes choose to attach.
The impact of interference drag on attached remora can also be taken into account through a well-resolved CFD analysis. We used remora body geometry from a CT scan and performed an additional CFD simulation with a remora attached at 3 of the 32 locations (Fig. 6). We also used CFD to calculate the rigid body drag of the remora in the freestream. We then compared our empirical result at the same locations with this full CFD approach. Our CFD predicted a drag reduction of 17% at S3 in a remora of about 0.2 times hmax in height (Fig. S4). Our empirical method predicted 42%. It could be argued that this suggests that the empirical method overpredicts drag reduction, but the accuracy of the CFD approach is limited by the grid resolution of the simulated remora due to the large size of the whale and therefore lower resolution of the computational grid at the scale of the remora. We hold that the fact that the two methods are reasonably close and both predict drag reduction is compelling complementary support of the results of our empirical approach, and thus our hypothesis. Our data show that remora congregate in S and W regions, where there is more drag reduction available, and not in NR regions, where there is far less drag reduction.
Particle image velocimetry (PIV) of a remora (R. australis) within a few centimeters of a surface revealed another physical phenomenon that might be important for helping remoras return to contact with their hosts while changing position. As a remora approaches a host surface (Fig. 7A), the narrowing at the entrance to the space between the remora and the host surface results in an acceleration of fluid so that flow between the remora and the surface is faster than freestream (Fig. 7B). Therefore, the fluid pressure between the remora and the surface is possibly lower than the surrounding fluid – a phenomenon known as the Venturi effect. The resulting suction effect may help the remora stay close to the host while it is temporarily detached.
Some remoras, such as R. australis, are known to be specific to cetaceans but other host-generalist species, such as Echeneis naucrates, also frequently attach to whales and dolphins, among others (Fertl and Landry, 1999; O'Toole, 2002; Silva-Jr and Sazima, 2003). Remora attachment locations on host organisms are known primarily through fishery landing reports, photographs and anecdotal information. By using opportunistically recorded camera-tag video data, the work presented here is the first continuous recording of remora behavior on a host organism, allowing for behavioral observations of individual remoras for extended periods of time. Our work provides strong evidence that remora preferentially select regions offering lower drag and, in particular, regions near separation of flow caused by surface features and in the wakes that develop downstream of those regions. While remoras were observed in other locations that offered little to no drag benefit, those individuals were fewer in number, typically smaller in size, and were in some cases observed later moving to a region offering lower drag. Interestingly, remoras were not observed on the ventral surface of the whales, which might be a location with the advantage of being less susceptible to sea bird predation (Sazima, 2018). Moreover, during surfacing, remoras maintained their attachment on the dorsal aspect of the whales and did not detach or move to maintain a submerged, and potentially more protected, position.
We observed behaviors in remora that we refer to as ‘skimming’ and ‘sliding’. Skimming, or swimming and gliding very close to the whale surface, allows the remora to change to a more lateral or caudal position with little to no swimming effort, and to swim upstream with reduced effort if the remora is small enough and close enough to the whale to experience drag reduction. In general, however, there is very little drag reduction while skimming and the remora most likely take advantage of their streamlined shape to skim with ease. When skimming within a couple of centimeters of the surface of the whale, the Venturi effect may, in part, help the remora maintain proximity to the whale while traveling parallel to the whale body, which facilitates reattachment and, in some regions, helps to keep the remora in the reduced flow of the boundary layer. Of course, the curved ventral surface of the remora is expected to lead to a low-pressure zone, and thus lift, that would tend to pull the remora away from the whale. So, a low-pressure region between the whale and a skimming remora may simply counteract that lift force.
The sliding behavior of remoras involves two mechanisms that contribute to remora adhesion: suction and friction. Remora adhesion is accomplished when the dorsally located adhesive disc is pressed against a host surface and serially arranged bony lamellae rotate. The outer edge of the disc is composed of a fleshy lip that seals the disc to the host surface and a suction force is generated by the negative pressure space created by lamellar rotation (Beckert et al., 2015; Fulcher and Motta, 2006). Aside from friction due to the contact of the two surfaces, additional resistance to shear is generated when small pointed spinules on the lamellae engage with local surface roughness of the host. Friction force contributed by the spinules has been estimated by measuring the shear force needed to slide a remora along rough and smooth surfaces (e.g. Plexiglas; Beckert et al., 2015; Fulcher and Motta, 2006; Gamel et al., 2019). One study measured the maximum shear forces at adhesive failure to be 17 and 11 N on rough and smooth surfaces, respectively, showing that the spinules contribute significantly to attachment via friction (Beckert et al., 2016b; Fulcher and Motta, 2006). We calculated shear forces on remora attached to a whale swimming 1.5 m s−1 to be no more than about 0.3 N, well below either of these values. Even on a whale swimming at 3.9 m s−1, we would expect drag of no more than 2–4 N. This suggests that while we found remoras to locate in low drag regions on a whale, they are probably capable of remaining attached almost anywhere on even a fast-swimming whale. Their selection of low drag regions is therefore not a necessity as much as an advantage that would decrease energy needed to remain attached and, to some extent, move about the whale surface. This makes sense of our observation that remora occasionally attached to the whale's tail where they would certainly experience high drag at times.
As remoras stay in contact with the whale surface during sliding, it seems that some suction is maintained while the lamellae may not be rotated enough to create significant spinule contact with the surface. This suggests that remoras can actively modulate the position of their lamellae and still maintain attachment to a host surface while under shear conditions. This ability could be important for clearing fluid out of the disc while attached, especially if seep due to the pressure difference between the inside and outside of the disc could causes suction loss (Beckert et al., 2016b; Flammang and Kenaley, 2017).
Our work represents the highest resolution whole-body fluid dynamic analysis to date of the world's largest vertebrate. Knowledge of blue whale hydrodynamics has broad-reaching implications for understanding their ecology and physiology, especially with consideration to analyzing the metabolism of a migratory animal. For example, this will inform the study of energetic expenditure in swimming, prey reactions to turbulent flow around a predator, and maneuverability, which depends on forces generated by control surfaces. In addition, understanding the flow environment around a swimming blue whale could help inform the placement of tags and new technology so they are more likely to remain attached to the animal (Gamel et al., 2019). Current tag technology, like that used to collect the video data used here, are restricted to suction cup attachments, which can fail within hours and rarely remain attached for more than 48 h. It is our hope that better understanding of both the flow environments around an organism and the mechanisms by which specifically adapted organisms like remoras successfully attach to hosts will help to inspire new technologies and designs for behavioral and ecological monitoring.
We are extremely thankful for the support and contributions of the following people: S. Soletto and Z. Zahid assisted in spotting remoras in videos and preliminary theoretical drag calculations; L. Natanson, NOAA Fisheries, provided frozen remoras of unusual size; L. Winn worked on an initial scan of the blue whale model; I. Malcom, University of Texas Austin, gave insightful comments.
Conceptualization: B.E.F.; Methodology: B.E.F., S.M., O.L., E.J.A., D.E.C., J.C., A.S.F., J.A.G.; Software: G.H., M.V., D.E.C. Formal analysis: B.E.F., S.M., O.L., E.J.A., A.M., D.E.C. Investigation: B.E.F., S.M., O.L., E.J.A., M.B., J.H.N.; Resources: O.L., E.J.A., G.H., M.V., J.C., A.S.F., J.A.G.; Data curation: D.E.C., H.E.A.; Writing - original draft: B.E.F., S.M.; Writing - review & editing: B.E.F., S.M., O.L., E.J.A., D.E.C., M.B., J.H.N., H.E.A., J.C., A.S.F., J.A.G.; Visualization: B.E.F., O.L., E.J.A., A.M.; Supervision: B.E.F.; Project administration: B.E.F.; Funding acquisition: B.E.F., S.M., E.J.A., J.A.G.
Financial support was provided by Stanford University and a Terman Fellowship to J.A.G., the Ministerio de Economía y Competitividad, Secretaría de Estado de Investigación, Desarrollo e Innovación, Spain (ref. TRA2017-88508-R) to O.L., a grant from the Grove City College Swezey Fund to E.J.A., and New Jersey Institute of Technology to B.E.F. Additional support for tagging and filming operations came from the National Oceanic and Atmospheric Administration (NOAA) and the BBC. The Barcelona Supercomputing Center awarded access to the MareNostrum IV machine (Barcelona, Spain).
The authors declare no competing or financial interests.