Immunosenescence is a well-known phenomenon in mammal systems, but its relevance in other long-lived vertebrates is less understood. Further, the influence of age and reproductive effort on immune function in long-lived species can be challenging to assess, as long-term data are scarce and it is often difficult to sample the oldest age classes. We used the painted turtle (Chrysemys picta) to test hypotheses of immunosenescence and a trade-off between reproductive output and immune function in a population of a long-lived vertebrate that has been monitored for over 30 years. These long-term data were utilized to employ a unique approach of aging turtles with mark–recapture data and population-specific growth modeling to obtain more accurate estimates of age. We analyzed natural antibodies, lysis ability and bactericidal competence in 126 individuals from 1 to 33 years of age captured during May and June 2011. Older turtles exhibited greater natural antibody levels than young individuals. Young females with large clutches exhibited greater lysis ability, while older females with large clutches had decreased lysis ability, suggesting a trade-off between reproductive output and immune function conditional upon age. However, bactericidal competence increased later in the nesting season for older females. Our study rejects the hypothesis of immunosenescence in a long-lived turtle, despite evidence of actuarial and reproductive senescence in this population. Additionally, we detected mixed evidence for a trade-off between reproduction and immune health.
Vertebrate aging is most commonly characterized by declining reproduction and survival with advancing age. Immunosenescence, age-related changes in composition and function of the immune system in vertebrates, is a potential mechanism by which senescence shapes population demography. Immune defenses are energetically costly, but reduced immune system function lowers defenses to parasitism and disease, which could decrease survivorship, reproductive ability or parental care in older individuals (Schmid-Hempel, 2003; Palacios et al., 2011b). Immunosenescence has been well studied in humans, where impacts of advancing age have been found in both the innate and adaptive branches of the human immune system (Pawelec et al., 2010). The complex relationship between age and immune function in other vertebrates is less understood, in part because of the difficulty of maintaining long-term datasets to reliably age long-lived vertebrates and obtain confident representation of the oldest age classes where senescence patterns are often most pronounced (e.g. Reed et al., 2008; Warner et al., 2016).
The change in immune function with age in wild vertebrates is hypothesized to be dependent on the life history of an organism (Nussey et al., 2013). Long-lived species may invest more in adaptive immune responses, which take longer to develop than innate responses yet are more effective against repeated pathogen exposure, while short-lived species may rely on less energetically expensive innate immune responses (Lochmiller and Deerenberg, 2000; Martin et al., 2006; Lee et al., 2008). Both immune system branches can senesce, at different rates, and this rate of senescence may be influenced by life history (e.g. Zimmerman et al., 2013b). Reproductive status and frequency also are predicted to influence immune function, such that increased reproductive investment occurs at the expense of immune function (e.g. Palacios and Bronikowski, 2017; Leivesley et al., 2019). Age may mediate the trade-off between reproduction and immune function, and older and younger individuals may express different immune responses to increased reproductive output. For example, the terminal investment hypothesis suggests that older individuals may respond differently to immune challenge than younger individuals, such that older individuals invest heavily in reproduction at the expense of immune function as their likelihood of future reproductive bouts decreases (e.g. Hanssen, 2006).
There are many gaps in our understanding of immunosenescence in reptiles. Many reptile species have long lifespans (30–70 years for turtles and crocodilians), grow past reproductive maturity and increase reproductive effort with size (Congdon et al., 2001; Wilkinson et al., 2016; reviewed in Hoekstra et al., 2020). These traits suggest that immunosenescence may not manifest in the same manner in reptiles when compared with other amniote vertebrates (mammals or birds) and may even be absent as a result of selective pressures on maintaining immune function with increased reproduction (e.g. Sparkman et al., 2007). Alternatively, reptile immune systems may experience trade-offs throughout their lives that prioritize either the less costly innate or relatively expensive acquired immune branches (Lee, 2006), dependent upon the age, sex, season or reproductive status of the individual. In reptiles, unlike mammals, the adaptive immune system responds more slowly and does not exhibit a robust response during repeated pathogen exposure (Zimmerman et al., 2010a). Thus, innate immune function is hypothesized to be critical to overall immune health. As individuals age, the innate immune system may be prioritized over the energetically expensive adaptive immune system, and this may result in the maintenance or even increase in function of the innate immune system with age, in contrast to aging mammals (Zimmerman et al., 2010a,b).
To increase our understanding of immunosenescence patterns and the influence of reproduction, sex and sampling date on immune function in a long-lived reptile, we studied the painted turtle, Chrysemys picta (Schneider 1783). Painted turtles are a widespread species throughout North America, and exhibit lifespans greater than 55 years in some populations (Congdon et al., 2003). While one study has assessed the immune function of painted turtles during the autumn, winter and spring (Schwanz et al., 2011), and another assessed the immune status of female painted turtles during the active nesting season (Refsnider et al., 2015), the variation in immune function between the sexes as they age is unknown. In this study, we sought to quantify the relationships among age, sex, reproductive output and the innate immune system during the nesting season (May–June) as an integrative approach to understanding immunosenescence in painted turtles. Specifically, we assessed three innate immune measures – natural antibodies, lysis ability and bactericidal competence of plasma – across age, sex and date during the nesting season in a well-studied population in eastern Illinois in the USA. This population of painted turtles has been studied for over 30 years, and represents a rare resource for studying senescence in reptiles. Additionally, female (and likely male) turtles in this population display increasing adult mortality with advancing age (Reinke et al., 2020; Miller et al., 2014), and older females have lower fitness than younger adult females (i.e. they produce eggs with reduced hatching success) (Warner et al., 2016). We hypothesized that painted turtles would demonstrate declining innate immune function, consistent with this recent evidence of actuarial and female reproductive senescence, and that the impact would be particularly pronounced in the oldest individuals. Additionally, we tested the hypothesis that reproductive effort negatively affects immune function, independent of age. This study therefore gives the first insight into the potential trade-offs between reproduction and immune system maintenance in turtles.
MATERIALS AND METHODS
Field methods and measures
The sampling for this study took place at a long-term research site, Thomson Causeway Recreation Area (TCRA), in Thomson, IL, USA. Painted turtle handling and sample collection were permitted by the United States Army Corps of Engineers, the United States Fish and Wildlife Service (SUP 32576-021) and the Illinois Department of Natural Resources (NH11.0073). This research was performed in accordance with the Iowa State University IACUC guidelines (12-03-5570-J). In May and June 2011, the nesting season for painted turtles in this population, we trapped painted turtles using fyke traps and terrestrial hand-capturing. We marked individuals caught for the first time (N=33) with a unique identifier and estimated age when possible using plastral scute rings (Moll, 1973). For individuals that were first captured and marked in previous years (N=93), we noted age based on plastral scute rings and previous capture history. We recorded six measurements of size for each turtle: curved carapace length and width, straight carapace length and width, and plastron length and width. We drew 200 μl of whole blood using sterile technique from the caudal vein for immune measures. Immediately following the blood draw, we separated the whole blood into plasma and red blood cells through centrifugation, and the plasma was placed into a separate tube and frozen in liquid nitrogen before storage at −80°C. We monitored nesting female painted turtles and processed them in a similar manner to trapped turtles. To measure reproductive output, we excavated nests and recorded clutch size for each nesting female. The 126 painted turtles we sampled for this study include reproductively mature males ≥3 years old (N=39) and females ≥6 years old (N=76) to 33 years old (see ‘Age determination’, below). Juveniles (N=11) were also included to represent the early stages of life before maturation.
Estimates of age for reptiles in immune studies have often relied on size as a proxy for age because of the difficulty of long-term monitoring of individuals across time and the general correlation between reptile age and size (e.g. Palacios et al., 2013; Zimmerman et al., 2017). While this proxy may be appropriate for general patterns of aging and immune function, growth curves can be sex specific, and in painted turtles, growth slows markedly at advanced ages, which complicates age estimation (Hoekstra et al., 2018). We incorporated new methods for aging the set of painted turtles in this study for whom age was unknown (35 out of 126). These new methods combine long-term capture history data and growth models to more accurately estimate individual ages for these cases.
We considered turtles to be of known age if they had visible, countable plastral scute rings at first capture, and these individuals were given an age equivalent to the number of scute rings (Moll, 1973). To calculate age in the study year (2011) for individuals of known age, we added the number of years that had elapsed since the first known-age capture. The age distribution of known-aged individuals in this study is shown in Fig. 1 (N=91). A recent investigation of painted turtle growth found that sex-specific von Bertalanffy growth models (VBGF) best describe the relationship between growth and age of individuals of known age in the population of turtles (Hoekstra et al., 2018). Thus, for the turtles we captured that had no recapture history, or whose initial capture age was questionable, we used a sex-specific VBGF to estimate age at first capture, and added subsequent elapsed years since first capture (N=35; Fig. 1). While the ages of several of the older turtles in this study were estimated, the maximum age estimated in this study (33 years) is reasonable given the reported known ages of turtles from this population (Hoekstra et al., 2018) and the recent recapture of female turtles in 2019 with known ages of 31 and 34 years and multiple males with known ages greater than 15 years. Additionally, the distribution of ages in this study is consistent with the distribution of ages reported in this population for males and females (Hoekstra et al., 2018).
Natural antibodies and lysis
Natural antibodies and lysis ability are proposed to be the first line of defense against pathogens in vertebrates (reviewed in Ochsenbein and Zinkernagel, 2000), and these aspects of innate immune function have been studied in many reptile species (e.g. western terrestrial garter snake: Sparkman and Palacios, 2009; painted turtle: Schwanz et al., 2011; water python: Ujvari and Madsen, 2011; desert tortoise: Sandmeier et al., 2012). In the autumn of 2011, we completed all measures of immune function on plasma samples. To assess natural antibody levels and lysis ability of plasma, we used a hemolysis–hemagglutination assay adapted from Matson et al. (2005) for use in painted turtles (Schwanz et al., 2011; Refsnider et al., 2015). Agglutination in this assay should be representative of natural antibody levels, which are abundant in a closely related turtle species, the red-eared slider turtle (Matson et al., 2005; Zimmerman et al., 2013a). Lysis may reflect activity of natural antibodies and activation of the complement system through natural antibodies, but the complement system may also be initiated by an alternative pathway involving cleavage of the C3 complement protein thioester, as has been found in snapping turtles (Baker et al., 2019). We deviated from the protocol used in painted turtles by using rabbit red blood cells (RRBC) instead of sheep red blood cells because hemolysis is not consistently observed with sheep red blood cells. We added 10 µl phosphate-buffered saline (PBS) to each well of a 96-well plate and added 10 µl positive control diluted 1:16 in PBS (anti-rabbit antibodies, MP Biomedicals; row 1), 10 µl plasma (rows 2–7) or 10 µl PBS (row 8) to the first column. We serially diluted twofold across two 96-well plates (columns 2–24). Finally, we added 10 µl of washed 3% RRBC in Alsever's anticoagulant (HemoStat Laboratories, Dixon, CA, USA) to each well. We incubated plates for 120 min at 26°C. Following incubation, we removed plates and tilted them for ease of scoring. After resting the plate at an angle for 20 min, we scored agglutination titer as −log2(1/D), where D represents the highest dilution at which agglutination occurred, and we gave half scores if the titer appeared intermediate. The first column for each sample was a 1:2 dilution of plasma; thus, the titer of the first column was scored as 2. We returned plates to the incubator at 26°C until 24 h after the initial plate incubation start time and scored lysis immediately following this incubation in the same manner as agglutination. We ran all plates with the positive and negative control and samples in duplicate where possible, with the exception of plasma-limited samples (N=10). We used only one bottle of RRBC in Alsever's anticoagulant for this assay, which was sourced from a single rabbit (personal communication from HemoStat Laboratories), to prevent any deviations in reactivity to RRBC across plasma samples. Agglutination and lysis ability were measured for 117 individuals. Higher titers for agglutination indicate greater abundance of natural antibodies in the plasma sample, as high titers are an indication that natural antibodies are at high concentrations even in increasingly diluted plasma (Matson et al., 2005). Similarly, high titers for lysis indicate the plasma is able to lyse RRBCs even at more dilute concentrations (Matson et al., 2005). Thus, increased natural antibody levels and lysis ability predict increased immune function.
Bactericidal competence of plasma
We assessed bactericidal competence of plasma by quantifying its ability to kill or inhibit growth of Escherichia coli using a method modified from Matson et al. (2006) and Palacios et al. (2011a) for use in painted turtles (Refsnider et al., 2015). We performed the assay in the same manner as Refsnider et al. (2015), with a few deviations noted here. We reconstituted a pellet of lyphophilized E. coli (Microbiologics, ATCC#8739) using 40 ml 1× PBS, and we further diluted the stock solution to a working concentration of 1:40, which produced approximately 150 colony-forming bacteria per 10 μl. We performed three control reactions by adding 10 μl of the bacterial working solution to 100 μl PBS. Sample reactions consisted of 10 μl plasma, 90 μl warm PBS and 10 μl E. coli working solution, and both control and sample reactions were incubated for 20 min at 28°C. We plated the sample reactions and the three control reactions in duplicate using 50 μl aliquots on 4% tryptic soy agar and incubated the plates overnight at 28°C. For each sample and the controls, we averaged bacterial colony counts between the duplicates, and divided this average by the average number of colonies on the control plates. This value was subtracted from 1 and multiplied by 100 to obtain percentage bacteria killed or inhibited from growth. Bactericidal competence was assessed in 105 individuals. Increased bactericidal competence predicts increased immune function.
We performed the following statistical analyses in SAS v. 9.4 (SAS Institute, Cary, NC, USA). We used a threshold of three standard deviations from the mean for outliers in the immune variables and subsequently removed two outliers from the agglutination results. As there were occasional negative percentages of bactericidal competence due to minor variations in bacterial density from the working stock solution, we removed all negative percentages of bactericidal competence from the dataset if the percentage was less than −10% (N=12), and we adjusted percentages between 0% and −10% to zero (N=7) to represent no bactericidal competence. Thus, final sample sizes for the following models were 115 for natural antibody levels, 117 for lysis ability and 93 for bactericidal competence. We assessed normality for natural antibodies, lysis and bactericidal competence and found that while natural antibodies and lysis were normally distributed, bactericidal competence was not. Transformations failed to achieve normality; thus, we analyzed untransformed bactericidal competence. We z-transformed age for each sex grouping with mean zero and unit variance (hereafter ‘zAge’) for male, female and juvenile painted turtles, as painted turtles in this population exhibit different distributions of age (mean±s.d. age: females 14±6.39 years, males 8.36±3.26 years, juveniles 2.22±0.77 years). Juveniles, while not a sex class, were thus treated as a separate sex for the models, as z-transforming age removed the correlation between sex and age, and juveniles display characteristics separate from those of sexually mature adults.
This model only assessed reproductive females in our dataset for which we have clutch size data (N=62, ages 6–33 years). As only females were included in this model, corrections for sex-specific age distributions are not needed, and thus we used age in years instead of zAge. For the agglutination model, we removed all interactions, as they were non-significant (P>0.1). Some interactions were significant for the female lysis and bactericidal competence models, and were thus included in the final models. We discuss model results in terms of least squares means (LS mean) ±s.e.m. We created all figures with ggplot2 (Wickham, 2016) in R (http://www.R-project.org/). To visualize significant interactions among continuous variables from each model, we calculated minimum, maximum and quartiles of continuous variables and calculated LS mean estimates at these values from the model. We then plotted these LS mean estimates ±s.e.m.
Finally, we assessed the influence of the oldest turtles for which we had estimated ages from sex-specific VBGF, rather than known ages, on model estimates (Fig. 1). For individuals with an estimated age (N=35), we replaced the age we estimated from sex-specific VBGF with the minimum age possible given the number of years elapsed since first capture and age at sexual maturity (3 years for males, 6 years for females). This is a conservative estimate of age for individuals whose age was not known. We found that the model results using minimum age did not differ from the models including age estimated from sex-specific VBGF, and thus we used the dataset including known age and estimated age from sex-specific VBGF.
There were no significant correlations among natural antibodies, lysis ability and bactericidal competence of plasma. Level of natural antibodies and lysis ability of the plasma samples were not correlated (Pearson's r=−0.09, P=0.30), and bactericidal competence was not correlated with either natural antibodies or lysis ability (Pearson's r<0.2, P>0.05).
The results of models of the relationships between zAge, sex and day of year and the dependent variables across all individuals included in this study are summarized in Table 1. The full model of natural antibody levels versus zAge, sex and day indicated significant effects of all three variables on natural antibodies (Table 1). Natural antibodies were significantly different between the sexes, with females displaying greater average agglutination titers, or greater natural antibody levels, than males (LS means±s.e.m.: females 18.31±0.32, males 16.74±0.41, P=0.0039), and juveniles having intermediate titers between the two sexes (17.82±0.76). To assess the effect of zAge and day of year on natural antibodies, we calculated residuals from the model excluding zAge or excluding day, respectively, and assessed the regression of residuals on the dependent variable. Natural antibodies increased with age (Fig. 2) and decreased with sampling date (y=−0.06x+9.64; R2=0.04). The results for lysis ability indicated a marginally significant difference between sex groupings, with males having the highest lysis ability (3.78±0.20), juveniles the lowest (2.91±0.38), and females having intermediate lysis ability (3.27±0.16; Table 1). There were no significant effects of zAge, sex or day of year on bactericidal competence (P>0.3, Table 1).
The models testing the relationship between female reproductive output and the immune measures are summarized in Table 2. For the subset of female individuals for which clutch size was known, age, day of year and clutch size did not significantly explain natural antibody levels (all P>0.1; Table 2). Lysis ability was affected by an interaction between clutch size and age (P=0.01; Table 2). As clutch size increased, lysis ability also increased for all ages except the oldest females (Fig. 3). For the oldest quartile of females (age >18 years), in contrast to all other ages, lysis ability decreased with increasing clutch size. Both clutch size and age interacted with day to affect bactericidal competence of plasma (Table 2). For most of the female nesting season, bactericidal competence was of similar magnitude across clutch sizes. Late in the nesting season, however, females with larger clutches had increased bactericidal competence. Bactericidal competence decreased with day of year for most ages except the oldest females, whose bactericidal competence increased with day (Fig. 4).
We found that, contrary to our predictions, painted turtles did not exhibit declining innate immune function with age, despite evidence of actuarial senescence (increasing mortality with increasing adult age) in this population (Reinke et al., 2020; Warner et al., 2016). In contrast, we found that the oldest animals within each sex category had increased circulating natural antibodies (Fig. 2). There were, however, age-related impacts on female immune function. The oldest reproductive females had decreasing lysis ability with increasing reproductive effort (Fig. 3). However, these same old females had increasing bactericidal competence across the nesting season (Fig. 4), in contrast to all other age classes of females. Thus, the evidence for a cost of reproduction is mixed at best.
Reptiles represent an important vertebrate group in our understanding of senescence, as most exhibit growth throughout their lifetime and demonstrate increasing reproductive output with age. However, our results and the results of the few studies of immunosenescence in non-avian reptiles are varied (reviewed in Vogel et al., 2018). For example, in populations of terrestrial garter snake (Thamnophis elegans), both natural antibodies and lysis ability decreased in older snakes (Sparkman and Palacios, 2009), while in water pythons (Liasis fuscus), natural antibodies increased with age (Ujvari and Madsen, 2011). In Dickerson's collared lizards, immune response to a mitogen, phytohemagglutinin, declined with age (Plasman et al., 2019). In red-eared slider turtles (Trachemys scripta) and painted turtles, the results are similarly inconsistent. When using plastron length as a proxy for age, larger adult red-eared slider turtles exhibited increased natural antibody levels when compared with smaller adults (Zimmerman et al., 2010b, 2013a). However, bactericidal competence declined with age, as did a number of adaptive immune measures: specific antibody responses to lipopolysaccharide challenge and B-cell function were maintained with age, while white blood cell count declined with age (Zimmerman et al., 2010b, 2013b, 2017). In painted turtles, one study found increased swelling response and decreased basophil count in larger individuals, but no change in natural antibody level with size (Schwanz et al., 2011). Our results, however, indicate that older painted turtles had higher levels of natural antibodies than younger individuals within each sex (males, females and juveniles). This is likely because of increased sample sizes in this study, a more confident age estimate, and a different sampling season, which can impact natural antibody levels (Schwanz et al., 2011).
There are multiple explanations for the inconsistency across measures in immunosenescence results among reptiles, including our study. First, reptiles may not follow the classic expectation of immunosenescence because of their life-history traits, such as indeterminate growth and, in the case of some chelonians, extreme lifespans compared with other vertebrates (e.g. Galapagos tortoises). Body size is positively correlated with reproductive output in many reptiles, which may allow for the evolution of decreased or negligible immunosenescence in long-lived species such as turtles (reviewed in Hoekstra et al., 2020). Our results are mostly in agreement with the maintenance of immune function with age. However, there is evidence of immunosenescence in certain immune measures both in the animals in our study and in other reptiles (reviewed in Vogel et al., 2018). Another explanation for the inconsistency in immunosenescence results is that immune components are likely to respond differently to age. The measures in this study, natural antibody levels, bactericidal competence and lysis ability, are responses of the innate immune branch rather than the specific, adaptive immune branch of the humoral immune system in reptiles (though natural antibodies may function as an innate and adaptive response; Vogel et al., 2018). In reptiles, adaptive humoral immune responses take longer to reach peak antibody concentrations after infection and exhibit no increased binding affinity to antigens upon secondary infection, in contrast to mammals (Zimmerman et al., 2010a). Thus, innate responses should be strongly selectively maintained with age in reptile immune systems as a first line of defense to compensate for less robust adaptive immune responses (Zimmerman et al., 2013a). Natural antibodies have been shown to increase with age in many reptiles, while components of the adaptive immune system displayed decreased responses in older individuals, consistent with a trade-off between innate and adaptive immune branches with age (Ujvari and Madsen, 2011; Zimmerman et al., 2013b).
Sex-specific immune function
We identified sex-specific differences in adult painted turtles in this study, such that males have lower natural antibody levels than females. This finding is unique, as other studies of turtle natural antibodies have found no difference between the sexes (Zimmerman et al., 2010b, 2013a,b; Schwanz et al., 2011). Sex-specific differences in vertebrate immune function are common and are explained by the action of androgens and behavioral differences between males and females (Klein, 2000). Males often exhibit reduced immune function, particularly in polygynous species, and experimental studies suggest that testosterone is a suppressive agent (reviewed in Foo et al., 2017). Behavioral differences in intraspecific interactions and habitat choice may subject males to greater infection pressures than females, which may also suppress male immune function (Klein, 2000). Painted turtle males are polygynous, have differing parasite loads and basking behavior than females in some populations, and demonstrate increasing testosterone beginning in mid-May with a peak in the autumn (Esch and Gibbons, 1967; Licht et al., 1985; Ernst and Lovich, 2009). These characteristics, and the intermediate natural antibody levels found in juvenile painted turtles in this study, support a role for increased testosterone and behavioral differences in suppressing male painted turtle immune function. However, lysis ability was greater in male than in female painted turtles, though the finding was only marginally significant, and bactericidal competence did not indicate sex-specific differences, which suggests that certain immune components may not be impacted by testosterone or behavioral differences, or that our study may not have captured sex-specific immune differences because of limited seasonal sampling. Indeed, in the turtle Mauremys caspica, there were sex-specific seasonal differences in multiple innate and adaptive immune measures (Muñoz et al., 2000).
Effects of day of year on immune function
Seasonality of immune function has been documented across vertebrates, and is predicted by cyclical activity of steroid hormones and reproduction, resource availability and temperature changes through the year (Martin et al., 2008; Moeller et al., 2013). In ectothermic vertebrates, temperature is particularly important to immune function, and multiple studies have found changes to immune function during or just following winter dormancy in reptiles (Muñoz et al., 2000; Schwanz et al., 2011; Sandmeier et al., 2016). Sampling for this study took place in May and June, which correspond with heightened female activity associated with nesting behaviors, increased basking behavior and male movement to find mates and new territories (Ernst and Lovich, 2009). Additionally, these months represent a marked increase in temperature. In 2011, the average maximum May temperature was 21.5°C in Thomson, IL, USA, and the average maximum June temperature was 26.8°C (https://daac.ornl.gov/DAYMET/). A study of a Michigan population of painted turtles found that adults had increased parasite load in warmer temperatures compared with cooler temperatures (Esch and Gibbons, 1967), and parasite pressures may also differ with temperature in this population.
While this sampling period is relatively short for understanding seasonal patterns in immune function, we still detected associations between sampling day and immune measures. Natural antibodies decreased with day of year, which may be a function of a decrease in immune health from female reproductive energy costs and an increase in male testosterone. There was also a significant interaction between age and sampling day for females with clutch information, such that all but the oldest females exhibited declines in bactericidal competence later in the nesting season (Fig. 4). However, bactericidal competence across all individuals did not correlate with day in this study, which differs from seasonal changes detected in other turtle species. Adult red-eared slider turtles from a population in Illinois demonstrated a marked drop in bactericidal competence in late June, while Mojave desert tortoises (Goperhus agassizii) increase bactericidal competence from spring to autumn (Zimmerman et al., 2010b; Goessling et al., 2016; Sandmeier et al., 2016). The different findings of seasonal impacts on bactericidal competence in other turtles versus this study may be predicted by trade-offs in energetic balance and the seasonal life cycles of parasites that are different between species and may invoke distinct immune responses to season across both species and immune components (Martin et al., 2008; Martinez-Bakker and Helm, 2015).
Reproductive output and immune function
Reproduction represents a large energetic cost to females. Life-history theory predicts that individuals invest heavily in current reproduction only at the expense of future reproductive opportunities and self-maintenance (Roff, 2002). Thus, a trade-off between immune function and reproduction might be expected, such that increased reproductive effort results in a decline in immune function. This trade-off has been experimentally tested in brown anoles (Anolis sagrei), and females that were reproductively active displayed lower immune function than ovariectomized females (Cox et al., 2010). Painted turtles exhibit a positive relationship between size and egg mass, suggesting increased reproductive effort with age (Congdon et al., 2003; Warner et al., 2016). In this study, older females with larger clutches had a lower lysis ability than younger females with large clutch sizes (Fig. 3). This is consistent with an age-dependent trade-off, as older females face an increased cost of immune function with increased reproductive effort. While little is known about the transfer of antibodies in turtles, in the desert tortoise, mothers transferred specific antibodies to their eggs and conferred resistance to a specific bacterial infection (Schunlacher et al., 1999). If innate immune function of mothers affects yolk provisioning in eggs, this may partially explain the reproductive senescence (reduced egg hatching success) reported for older females in this population (Warner et al., 2016).
In contrast, bactericidal competence of older females increased through the nesting season, while younger females exhibited a significant decrease (Fig. 4), again rejecting the hypothesis of immunosenescence. This increase in older females throughout the nesting season suggests that reproduction is not negatively impacting all aspects of immune function. Painted turtles have been commonly recorded laying second clutches in this population, and older females tend to lay second clutches more often than young females (Bowden et al., 2011). While this additional increased reproductive output may contribute to the lowered lysis ability for older females with large clutches, the increased bactericidal competence suggests that older females may be better able to respond to immune challenges later in the nesting season, when second clutches are laid. These results add to the growing research that suggests not all immune measures are equally influenced by age, reproduction and season.
This study is a valuable contribution to our understanding of variation in immune function across age, sex and reproductive effort in a long-lived reptile. Long-term studies of wild reptile populations allow the direct aging of individuals, which offers insight into senescence patterns that occur only in the oldest individuals. While immunosenescence was not supported in most measures of immune function in the painted turtle, we found interactions between sex, date and age that emphasize the importance of physiological state in immune function. Additionally, we report the first evidence of immunosenescence associated with reproductive output in older females in a turtle, which supports the life-history trade-off between reproduction and self-maintenance in this long-lived reptile.
We thank the many members of the Janzen lab for contributions to the collection of long-term data at this site and, especially, to the collection of samples in 2011. We also thank Luke A. Hoekstra and David A. Miller for assistance in estimating age with different growth models.
Conceptualization: A.M.B., D.M.R.; Methodology: A.M.B., D.M.R.; Validation: J.M.J., A.M.B., D.M.R.; Formal analysis: J.M.J., A.M.B.; Investigation: J.M.J., A.M.B., D.M.R.; Resources: J.M.J., A.M.B., D.M.R.; Data curation: J.M.J., A.M.B.; Writing - original draft: J.M.J.; Writing - review & editing: J.M.J., A.M.B., D.M.R.; Visualization: J.M.J.; Supervision: A.M.B.; Project administration: A.M.B.; Funding acquisition: A.M.B.
Funding for fieldwork was provided by National Science Foundation grants LTREB DEB-0640932 and DEB-1242510 awarded to Fredric J. Janzen. Support for age estimates and additional fieldwork funding was provided by the National Institute on Aging (R01AG049416) awarded to A.M.B. Deposited in PMC for release after 12 months.
Data, including results for all immune assays and SAS code used to perform statistical analyses, are available from Iowa State University's data repository (hosted by figshare): https://doi.org/10.25380/iastate.12290585
The authors declare no competing or financial interests.